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Wikipedia:Featured picture candidates/Actin Filaments in a Cultured Cell

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Voting period is over. Please don't add any new votes. Voting period ends on 11 Jun 2015 at 00:25:04 (UTC)

Original – A z-projection of an osteosarcoma cell that was stained with phalloidin in order to visualise actin. Image was deconvoluted using an experimental point spread function and colour coded in the z-axis to better show the 3D position of the filaments.
Reason
Very high technical standard, high resolution image, gives insight into the complexity of cells (this is the network formed by just one of the cell's cytoskeletal proteins) aesthetically pleasing, has scale and metadata.
Articles in which this image appears
Deconvolution, Confocal microscope, Fluorescence microscope
FP category for this image
Wikipedia:Featured pictures/Sciences/Biology
Creator
methylated603
  • @Mattximus: Thanks, yeah I used phalloidin (it would have conjugated to a 488 fluorophore) and took images every 80nm from the bottom to the top to get what the filament distribution is like in the whole cell. To show it in 2D I've depth coded it then merged it so each slice in 'z' is a slightly different colour allowing you to see where abouts the filaments are with regards to the cell height. If you look closely at the full resolution image you'll see that there is posterization in some places, this is intentionally left as thats where one slice ends and the next begins. I took this on an inverted microscope, the orange/red is at the cell base (what was attached to the coverslip) and the purple is at the very top of the cell. Methylated603 (talk) 05:20, 1 June 2015 (UTC)[reply]
What's the measurement – mm.? Sca (talk) 15:27, 1 June 2015 (UTC)[reply]
Micrometre Mattximus (talk) 23:18, 1 June 2015 (UTC)[reply]

Promoted File:Depth Coded Phalloidin Stained Actin Filaments Cancer Cell.png --Armbrust The Homunculus 02:52, 11 June 2015 (UTC)[reply]