TraJ-II RNA motif
| traJ-II RNA | |
|---|---|
 Consensus secondary structure of traJ-II RNAs | |
| Identifiers | |
| Symbol | traJ-II |
| Rfam | RF01760 |
| Other data | |
| RNA type | Cis-regulatory element |
| Domain(s) | Pseudomonadota |
| PDB structures | PDBe |
The traJ-II RNA motif is a conserved RNA structure discovered in bacteria by using bioinformatics.[1] traJ-II RNAs appear to be in the 5' untranslated regions of protein-coding genes called traJ, which functions in the process of bacterial conjugation. A previously identified motif known as TraJ 5' UTR is also found upstream of traJ genes functions as the target of FinP antisense RNAs, so it is possible that traJ-II RNAs play a similar role as targets of an antisense RNA. However, some sequence features within the traJ-II RNA motif suggest that the biological RNA might be transcribed from the reverse-complement strand. Thus is it unclear whether traJ-II function as cis-regulatory elements. traJ-II RNAs are found in a variety of Pseudomonadota.
It was later observed[2] that traJ-II RNAs overlap a previously established oriT (Origin of transfer) plasmid sequence,[3][4] thus suggesting that the traJ RNA motif might function as an oriT.
References
[edit]- ^ Weinberg Z, Wang JX, Bogue J, et al. (March 2010). "Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea and their metagenomes". Genome Biol. 11 (3): R31. doi:10.1186/gb-2010-11-3-r31. PMC 2864571. PMID 20230605.
- ^ Li Y, Cunha da Silva G, Li Y, Rossi CC, Fernandez Crespol R, Williamson SM, Langford PR, Soares Bazzolli DM, Bossé JT (October 23, 2018). "Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae". Front. Microbiol. 9: 2489. doi:10.3389/fmicb.2018.02489. PMC 6206304. PMID 30405558.
- ^ Ziegelin G, Fürste JP, Lanka E (July 1989). "TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin". J. Biol. Chem. 264 (20): 11989–11994. doi:10.1016/S0021-9258(18)80164-8. PMID 2663846.
- ^ Pansegrau W, Balzer D, Kruft V, Lurz R, Lanka E (September 1990). "In vitro assembly of relaxosomes at the transfer origin of plasmid RP4". Proc. Natl. Acad. Sci. U.S.A. 87 (17): 6555–6559. Bibcode:1990PNAS...87.6555P. doi:10.1073/pnas.87.17.6555. PMC 54575. PMID 2168553.
External links
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