N-acylglucosamine 2-epimerase
| N-acylglucosamine 2-epimerase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 N-acylglucosamine 2-epimerase dimer, Sus scrofa | |||||||||
| Identifiers | |||||||||
| EC no. | 5.1.3.8 | ||||||||
| CAS no. | 37318-34-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a N-acylglucosamine 2-epimerase (EC 5.1.3.8) is an enzyme that catalyzes the chemical reaction
- N-acyl-D-glucosamine N-acyl-D-mannosamine
Hence, this enzyme has one substrate, N-acyl-D-glucosamine, and one product, N-acyl-D-mannosamine.
This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is N-acyl-D-glucosamine 2-epimerase. Other names in common use include acylglucosamine 2-epimerase, and N-acetylglucosamine 2-epimerase. This enzyme participates in aminosugars metabolism. It employs one cofactor, ATP.
Structural studies
[edit]As of late 2019, three structures have been solved for this class of enzymes, with the PDB accession codes 1FP3, 2GZ6, and 6F04. They show that the N-acylglucosamine 2-epimerase monomer folds as a barrel composed of α-helices, in a manner known as (α/α)6-barrel. The structures are presented as dimers, with the structures from Sus scrofa and Anabaena sp. CH1 having a different organization than the structure from Nostoc sp. KJV10.
References
[edit]- Ghosh S, Roseman S (April 1965). "The sialic acids. V. N-Acyl-D-glucosamine 2-epimerase". The Journal of Biological Chemistry. 240: 1531–6. doi:10.1016/S0021-9258(18)97468-5. PMID 14285488.
