Alkane 1-monooxygenase
| alkane 1-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.15.3 | ||||||||
| CAS no. | 9059-16-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, an alkane 1-monooxygenase (EC 1.14.15.3) is an enzyme that catalyzes the chemical reactions
- an alkane + reduced rubredoxin + O2 a primary alcohol + oxidized rubredoxin + H2O.
Alkanes of 6 to 22 carbons have been observed as substrates.[1] This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with oxygen as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is alkane, reduced-rubredoxin:oxygen 1-oxidoreductase. Other names in common use include alkane 1-hydroxylase, omega-hydroxylase, fatty acid omega-hydroxylase, alkane monooxygenase, 1-hydroxylase, AlkB, and alkane hydroxylase. It contains a diiron non-heme active site.
Recently two crystal structures of the enzyme have appeared that provide much more information about the structure of the enzyme.[2][3] Both structures show an unusual diiron active site where the two iron ions are separated by more than 5 angstroms. Neither structure shows evidence for a ligand that would bridge the two iron ions.
References
[edit]- ^ McKenna EJ, Coon MJ (1970). "Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans". J. Biol. Chem. 245 (15): 3882–9. doi:10.1016/S0021-9258(18)62932-1. PMID 4395379.
- ^ Guo, Xue; Zhang, Jianxiu; Han, Lei; Lee, Juliet; Williams, Shoshana C.; Forsberg, Allison; Xu, Yan; Austin, Rachel Narehood; Feng, Liang (2023-04-17). "Structure and mechanism of the alkane-oxidizing enzyme AlkB". Nature Communications. 14 (1): 2180. doi:10.1038/s41467-023-37869-z. ISSN 2041-1723. PMC 10110569. PMID 37069165.
- ^ Chai, Jin; Guo, Gongrui; McSweeney, Sean M.; Shanklin, John; Liu, Qun (April 2023). "Structural basis for enzymatic terminal C–H bond functionalization of alkanes". Nature Structural & Molecular Biology. 30 (4): 521–526. doi:10.1038/s41594-023-00958-0. ISSN 1545-9985. PMC 10113152. PMID 36997762.
- Cardini G, Jurtshuk P (1970). "The enzymatic hydroxylation of n-octane by Corynebacterium sp strain 7E1C". J. Biol. Chem. 245 (11): 2789–96. doi:10.1016/S0021-9258(18)63058-3. PMID 4317878.
- Peterson JA, Kusunose M, Kusunose E, Coon MJ (1967). "Enzymatic omega-oxidation. II. Function of rubredoxin as the electron carrier in omega-hydroxylation". J. Biol. Chem. 242 (19): 4334–40. doi:10.1016/S0021-9258(18)99543-8. PMID 4294330.
