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Vg1 ribozyme

From Wikipedia, the free encyclopedia
Manganese dependent ribozyme in Vg1 mRNA
Secondary structure of the VG1 ribozyme.
Identifiers
SymbolVG1
RfamRF01865
Other data
RNA typeRibozyme
Domain(s)Xenopus
PDB structuresPDBe

The Vg1 ribozyme is a manganese dependent RNA enzyme or ribozyme which is the smallest ribozyme to be identified. It was identified in the 3′ UTR of Xenopus Vg1 mRNA transcripts and mouse beta-actin mRNA.[1] This ribozyme was identified from in vitro studies that showed that the Vg1 mRNA was cleaved within the 3′ UTR in the absence of protein. Studying the Vg1 mRNA 3′UTR a manganese-dependent ribozyme was predicted to exist. This ribozyme was shown to be located adjacent to the polyadenylation site and in vitro studies showed that it catalyzes a first-order reaction where its mechanism of cleavage is similar to the manganese ribozyme present in Tetrahymena group I introns.[2]

In vivo studies showed that this ribozyme is not functional with the cell.[1]

References

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  1. ^ a b Kolev NG, Hartland EI, Huber PW (October 2008). "A manganese-dependent ribozyme in the 3′-untranslated region of Xenopus Vg1 mRNA". Nucleic Acids Res. 36 (17): 5530–5539. doi:10.1093/nar/gkn530. ISSN 0305-1048. PMC 2553595. PMID 18753150.
  2. ^ Dange V, Van Atta RB, Hecht SM (May 1990). "A Mn2(+)-dependent ribozyme". Science. 248 (4955): 585–588. Bibcode:1990Sci...248..585D. doi:10.1126/science.2185542. PMID 2185542.
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