User talk:AssertiveClatter/Heterologous expression

Transfection

We may want to add information and link to the transfection page AssertiveClatter (talk) 17:08, 4 October 2022 (UTC)

Updates

I added all of the information from the doc we were working in. We will want to add into the sandbox going forward I think. We can discuss changes here.

I really think that the information that has been added is great, but that we need to work together to figure out the best layout for the page going forward as far as headings and subheadings, etc go. AssertiveClatter (talk) 17:10, 4 October 2022 (UTC)

I made a bunch of changes to the layout and some content edits, but I am still pretty concerned about what is now the first part of the methods section. It was something that was already in the article when we copied it over, but it was #1 on the numbered list in the lead section. It doesn't seem to fit and it has parenthetical numbers which make me think it came from another document that had references listed that never made it to the article. It could have been something the FIT student added, so I didn't want to necessarily remove it until we have discussed it. AssertiveClatter (talk) 04:11, 24 October 2022 (UTC)

Topic Peer Review 1

Introduction paragraph:

• Overall I thought the introduction was very informative and effective, I thought it did a great job of introducing a very intriguing method of gene expression and I enjoyed that it tied applications in protein interactions. There's a few edits I would suggest.
  • I would suggest moving the bit on “Techniques to isolate specified genes” and “Techniques to incorporate specified genes' ' to the methods section, I would also link them to their wikipedia links. Also link the citations.
  • “It provides a facile avenue to efficiently express and experiment with combinations of genes and mutants that do not naturally occur” was already mentioned in this first sentence so I would consider combining them.
  • I’m not sure what being conveyed in this sentence: “Another approach to isolating membrane proteins uses electrophoresis, a technique known as radiolabeled ligand or inhibitors is used(2)”
  • There was also a few typos:
    • “specified genes:Gene identification” should be “specified genes: Gene identification”

Methods:

• Again the methods section was effective for the most part but none of the techniques mentioned were linked, I suggest that if there is a wikipedia page on Gene gun delivery, Electroporation, Viral transduction or Lipofection then I would link them.
• I'm a bit confused as to how your methods sections directly correlate back to the discussion of heterologous expression.
• The last sentence in this section was already mentioned in the discussion

Limitations:

• Great section, I can’t really offer any suggestions other than maybe link some terms, like tRNA or the discussion on mutations.
• Grammar and typos
  • Glycosylation is spelled wrong
  • frame shift should be frameshift

Ethical Issues:

• I would move this section to the end before applications.
• The second paragraph also limitations, so maybe consider adding it to the previous section
• Cite some of the “many areas of active research addressing these limitations of utilizing heterologous expression”.
• Grammar and typographical errors:
  • “improved gene promoters” and “improving gene promoters”
  • “Post translational” should be “Post-translational”

Host systems

• This section has aspects of both the methods and limitations, so I would consider either combining this section with the methods and limitations or maybe finding somehow to connect them to make the overall article more coherent.
• I would also try tie in heterologous expression while talking about these host systems
• Grammar and typos
  • “Gram negative” should be gram-negative
    • Same with “gram positive” and “post translational”

Applications:

• Great section! Only a few issues
  • “A strain of E. coli was used in the making of the first heterologous protein product released to the market was human insulin, most commonly known as Humulin.” → poor sentence structure
  • Humulin should be linked to its wikipedia page (if there is one)

Overall presentation:

The information on this group's page about heterologous expression was quite interesting and easy to understand, so I definitely enjoyed reading it. Their introduction section is excellent and serves as a great foundation for the rest of the page by providing a thorough explanation of what heterologous expression is and why it is significant. Although there was some redundancy, the lengths of each segment are appropriate because they provide all the important information in the most concise manner possible. Their page includes references that are very beneficial and make it simple to learn more about the subject. The examples they provided regarding the effects of various methods and applications were really helpful in understanding the text. So overall a great job, but there were instances when I didn't understand how the information in your sections on methods, limitations, and ethical issues linked to the explanation of heterologous expression, so I would make sure I don't sidetrack to other topics. Nevertheless, the information and sources they offered were trustworthy, and relevant to the topic! oaabdullah~~~ Oaabdullah (talk) 01:13, 14 October 2022 (UTC)

Topic Peer Review 2

Heterologous expression

-The first sentence of this section reads a little off. Consider revising the end of the sentence.

-"native levels, to yield a specific product": there shouldn't be a comma here.

-"It provides a facile avenue to efficiently express": Consider leaving out 'efficiently' and 'facile' to improve neutrality.

-I think the last sentence could be combined with the lead paragraph as this will strengthen the intro with specific examples of how heterologous expression is used to study specific protein interactions.

Methods

-I love that you introduced Gene gun delivery, Electroporation, Viral transduction, and Lipofection in your lead section. Additionally, having separate paragraphs for each method gives this section a clear and organized structure.

-I like that the authors provided positives for each of the methods and how effective they are in vitro and in vivo. Perhaps the methods section could benefit from some downsides to each of the methods as well.

-The last sentence of this section should be deleted as it is identical to that in the lead section.

Limitations

-References are missing for the middle of this section. Reference #10 refers to the role of tRNA and how it creates problems in translating heterologous gene expression. However, much of the discussion before doesn't seem to correlate to that paper specifically. Other than that, I believe the authors did a thorough job in describing the limitations of heterologous expression.

-Could add to end of last sentence the phrase "which will be discussed below" to help improve readability

Ethical Issues

-"If genetic modification could be used freely by anyone, to change any aspect of their physical or mental form, it may eventually lead to severe discrimination": this sentence introduces strong bias, consider deleting. Linking to a source and revision would also help improve neutrality.

-When the authors say that CRISPR may cause further risks to the human body it would be beneficial to cite a specific example.

-https://www.bu.edu/khc/files/2018/10/CRISPR-Ethics-reading.pdf : Here is a paper about the ethical issues of CRISPR that the authors could use to support their conclusions. It also goes into how CRISPR harms the human body which can be used as a specific example.

-Strong ending and sentence structure towards the end of this section!

Host Systems

-It seems as if references are missing for a majority of this section?

-Revise grammar in this section

Applications

-The second sentence of this section (specifically "highly invasive procedures and the development of adverse drug reactions") makes it seem that heterologous expression has negative applications. Not sure if this is what the authors intended?

-The introduction and discussion of E-coli seems to be only tangentially relevant to the applications of heterologous expression- consider shortening or deleting.

-"Recombinant technology has also played a role in biofuel development". The topic of biofuel development comes suddenly. To improve the sentence structure and readability, start new paragraph with this sentence.

General comments

Overall, I think the authors did an excellent job in compiling information about heterologous expression, with many specific examples from past and more recent literature. I believe the article can be significantly improved in readability by fixing grammar, sentence structure, and transitions. It would also be beneficial to check the references and avoid tangents in certain sections to have a stronger theme across the article. Overall, great first draft and I have no doubt that with further revisions, that this will be an excellent addition to Wikipedia! Daniken01 (talk) 03:01, 14 October 2022 (UTC)

MLibrarian feedback

1) I would recommend linking mentioned concepts to other Wiki papers. For example, electroporation to https://wiki.riteme.site/wiki/Electroporation, Transduction to https://wiki.riteme.site/wiki/Transduction_(genetics), etc.

2) Please revise the grammar, I know some good suggestions were posted already

3) Sections assignment needs revision. For example, A) the first paragraph shall belong to no section, since it is a general description. All consecutive sections will be then main sections (1,2,3 etc), instead of being subsections (1.2, 1.2.,1.3, etc); B) Currently, "ethical issues" are listed as sub-section of limitations. I would suggest making it an independent section. Rephrase some sentences as was suggested before. And please rename "issues" to "concerns"; C) Limitations subsection - is it limitation to methods? Then, perhaps, it shall be a subsection of methods

4) In section Applications it is in plural but only one application is described below. Perhaps, you shall rename it to "Application in drug development" MLibrarian (talk) 01:59, 19 October 2022 (UTC)