User:Yinshuangg/Dissociation rate

The dissociation rate constant allows the calculation of the time during which a reversible proteinase/inhibitor complex remains undissociated in vivo in the presence of a dissociating agent (e.g. a biological substrate). In theory, the dissociation rate constant (kd) is given by the intercept on the ordinate when the slope of the association phase of dR/dt versus R is plotted against C. In practice, however, this intercept is too close to the origin to be determined with accuracy. Dissociation rate constants can be more reliably measured from the dissociation of the analyte from the ligand after buffer replaces the analyte solution.