User:Seaoli12oceans12/Brucella canis

Brucella canis is a gram-negative bacterium in the family Brucellaceae that causes brucellosis in dogs and other canids. B. canis is a non-motile zoonotic short-rod or coccobacillus organism, and is oxidase, catalase, and urease positive. B. canis has two distinct circular chromosomes that can attribute to horizontal gene transfer. B. canis causes infertility in both males and females. It can also demonstrate inflammation in the eyes. B. canis can range from domestic animals to foxes and coyotes. It is passed from species to species via genital fluids. Treatments such as spaying, neutering, and long-term antibiotics have been used to combat B. canis. The species was first described in the United States in 1966 where mass abortions of beagles were documented.

History[edit]

B. canis was discovered by Leland Carmichael in 1966, when the bacterium was identified in canine vaginal discharge and the tissues from mass abortions in beagles. B. canis was said to be a biovar of B. suis. With recent research, PCR assay data was able to contradict B. canis and B. suis. PCR data showed a complete difference between the two strains along with B. suis biovars unattained from B. canis DNA. PCR assays have been proven beneficial when differentiating between Brucella strains and vaccine strains.

Morphology/ Physiology

Brucella are non-motile meaning that they cannot move themselves through the environment, they must have assistance from environmental factors. This is due to the fact that B. canis does not produce flagella. Brucella are also non-encapsulated, non-spore forming bacteria that replicate in the Endoplasmic Reticulum of their host cells. They are gram negative and have a coccobacilli (short rod) shape. They demonstrate slightly rounded ends, with slightly outward curving sides. The bacteria form non-hemolytic, non-pigmented convex colonies on blood agar culture media. The optimal growth temperature for B. canis is 37 °C, but growth is still possible within the range from 20 °C to 40 °C. Additionally, the pH range in which B. canis grows most effectively is from pH 6.6 - 7.4, making this organism neutrophilic in nature.

Brucella have an unusual composition of fatty acids that make up their outer cell membrane. Myristic, palmitic, and stearic acids are all found in large quantities within the outer cell membrane. Cis-vaccenic and arachidonic acids are found in medium amounts, while C17 and C19 cyclopropane fatty acids are found in very limited amounts. Additionally, there are no hydroxy fatty acids present in Brucella outer cell membranes. This particular composition of fatty acids is believed to be the reason behind hydrophobic interactions that occur within the outer cell membrane and lead to greater cell stability.

B. canis is also unique from other Brucella species, in the fact that the lipids that make up its phospholipid portion, are mainly cis-vaccenic cyclopropane with small amounts of lactobaccilic acid. This differs from other Brucella species, as they demonstrate the opposite composition, with lactobacillic acid making up the majority of the phospholipid fraction. Overall, Brucella is unusual in this composition, due to the fact that lactobacillic acid is typically within gram-positive organisms but not common within gram-negative organisms such as Brucella.

Image of B. canis colonies growing on blood agar culture media.

Identification

B. canis is a zoonotic organism. The bacteria are oxidase, catalase and urease positive and non-motile. Unlike haemophilus, which they resemble, they have no requirements for added X (hemin) and V (nicotinamide adenine dinucleotide) factors in cultures. Full identification is established by serology and PCR. B. canis is not acid-fast, but they do tend to maintain their color when exposed to weak acids. This results in their red color when stained using Macchiavello's stain. When isolated, B. canis is always in a non-smooth or mucoid form. This non smooth form has highly hydrophobic LPS imbedded in its outer membrane, which is known to be soluble in phenol-hexane-chlorophorm, rather than phenol-water like smooth forms of Brucella are known to be.

Colonies of Brucella can typically start to be seen after 48 hours. These colonies tend to be 0.5-1.0 mm in diameter, with a convex shape and are typically circular. Mucoid variants such as B. canis have a sticky, glue-like texture, and have a variety of colors. B. canis can present with white, yellowish white, and even brown coloring. This is typical for both mucoid and rough Brucella variants.

Metabolism

B. canis functions as a chemoorganotroph, meaning it obtains its energy from oxidation-reduction reactions, and utilizes organic electron sources. B. canis utilizes oxygen as well as nitrate as its terminal electron acceptor within its electron transport chain. This electron transport system is known to function using cytochromes, and B. canis has the ability to utilize nitrate as a terminal electron acceptor due to the organism's ability to produce nitrate reductase.

While some Brucella species require supplemental CO2 for growth, B. canis does not. B. canis has demonstrated growth on media containing thionine, but no growth on media containing basic fuchsin.

Ecology

Brucella, including B. canis are obligate organisms, meaning they live intracellularly and do not ordinarily colonize outside of their host. While this is true, in very specific temperature and moisture levels Brucella can grow colonies in soil and surface water for extended time periods. These organisms exist in most regions around the world.

Genome

B. canis has two distinct circular chromosomes. These two circular chromosomes contain shared portions that can be attributed to horizontal gene transfer. The first of the two chromosomes is larger than the second, with an average of 2243 genes on chromosome 1, and 1229 on chromosome 2. Chromosome 2 is much smaller than chromosome 1. Chromosome 2 was derived from a plasmid, but both chromosomes contain genetic information necessary for survival, with these essential genes being split evenly between the two.

B. canis is thought to be a variant of B. suis Biovar 1, based on the genomic similarities between the two. The genomic structure of both B. canis and B. suis Biovar 1, cannot be distinguished from each other as they both demonstrate similar sizes within the two circular chromosomes present. Based on this similarity B. canis is thought to be a stable R mutant of B. suis Biovar 1.

Pathogenicity

Although there has been an increase in the international movement of dogs, Brucella canis is still very uncommon. The disease often presents as infertility in both male and female dogs, however it is characterized by epididymitis and orchitis in male dogs. Males face the chance of infertility, because they develop an antibody against their own sperm. This may be followed by inflammation of the testes which generally settles down after a while.  Endometritis, placentitis, and abortions of developed fetuses are the symptoms in female dogs. Other symptoms such as inflammation in the eyes, axial and appendicular skeleton, lymphadenopathy and splenomegaly are less common. Another symptom is the infection of the spinal plates or vertebrae, which is called diskospondylitis. It is generally spotted in the animal's reproductive organs.

Host Range

The host range of the B. canis is mainly domestic dogs but evidence of infections in foxes and coyotes have been reported. Although rare, humans can contract the infection. It is unlikely, but the most common human infections are in dog breeders, those in laboratories dealing with the bacteria, or people who are immunocompromised.

Transmission

B. canis is passed through contact with fluids from the mucous membranes of the genitals (semen and vaginal discharge), eyes, and oronasal cavities. This contact can occur during sexual activity as well as other daily grooming and social interactions.

High levels of B. canis exist in these secretions in the six weeks following abortion in females, and around six to eight weeks following infection in males. Lower levels of B. canis still remain in the semen of infected males for two years following infection, which can serve as a large source of transmission to other dogs.

Urine can also serve as a route of transmission in males, as the bladder resides in close proximity to the prostate and epididymus. This leads to contamination of the urine making it another vehicle for B. canis transmission.

Once entered into the host cell, Brucella canis can survive in phagocytic and non-phagocytic cells. B. canis utilizes virulence factors such as LPS (lipopolysaccharides), T4SS, and BvrR and BvrB. These virulence interact with the cell surface and the endoplasmic reticulum while the bacteria is multiplying.

Treatment

B. canis is relatively easy to prevent in dogs. There is a simple blood test that can be done by a veterinarian. Any dog that will be used for breeding or has the capability to breed should be tested.

Treatment is very costly and not always effective, as relapse is common in animals that have been infected. The most generally accepted course of treatment for brucellosis in canines is to avoid treatment altogether and opt for euthanasia. Dogs in kennels that are affected by B. canis are usually euthanized for the protection of other dogs and the humans caring for them. If treatment is the chosen pathway, a combination of antibiotics is thought to be the superior method. The combination of minocycline and streptomycin is also thought to be useful, but it is often unaffordable. Tetracycline can be a less expensive substitute for minocycline, but it also lowers the effect of the treatment. The combination of a tetracycline-based antibiotic and an aminoglycoside has had positive outcomes in previous studies. Despite positive clinical results, and reduction in the antibody concentrations for the infected animals, treatments did not result in the complete elimination of B. canis from the animals. Another combination of antibiotics that are effective include doxycycline with rifampicin and fluoroquinolones with rifampicin.

Long term antibiotics can be given but usually results in a relapse. Spaying and neutering can be effective, and frequent blood tests are recommended to monitor progress.