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Sea Lice
Male and female Lepeophtheiris salmonis
Scientific classification
Kingdom:
Animalia
Phylum:
Arthropoda
Subphylum:
Crustacea
Class:
Maxillopoda
Subclass:
Copepoda
Superorder:
Podoplea
Order:
Siphonostomatoida
Families

Caligidae

Diversity
36 genera, c. 500 species

Sea louse (plural sea lice) is the term generally used for species of copepods within the order Siphonostomatoida, family Caligidae. There are 36 genera within this family which include approximately 42 Lepeophtheirus and 300 Caligus species[1]. Sea lice are marine ectoparasites (external parasites) that feed on the mucus, epidermal tissue, and blood of host marine fish. In this article we focus upon the the genera Lepeophtheirus and Caligus which parasitize marine fish, in particular those species that have been recorded on farmed salmon. Lepeophtheirus salmonis and various Caligus species are adapted to saltwater and are major ectoparasites of farmed and wild Atlantic salmon. Several antiparasitic drugs have been developed for control purposes. Since L. salmonis is the major sea louse of concern and has the most known about its biology and interactions with its salmon host, this review will focus on this species. Caligus rogercresseyi has become a major parasite of concern on salmon farms in Chile[2] and studies are underway to gain a better understanding of the parasite and the host-parasite interactions. Recent evidence is also emerging that L. salmonis in the Atlantic has sufficient genetic differences from L. salmonis from the Pacific, suggesting that Atlantic and Pacific L. salmonis may have independently co-evolved with Atlantic and Pacific salmonids, respectively[3].


Diversity and hosts: Sea lice on wild fish

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Most of our understanding of the biology of sea lice, other than the early morphological studies, are based on laboratory studies designed to understand issues associated with sea lice infecting fish on salmon farms. Information on sea lice biology and interactions with wild fish is unfortunately sparse. Many sea louse species are specific with regards to host genera, for example L. salmonis which has high specificity for salmonids, including the widely farmed Atlantic salmon (Salmo salar). Lepeophtheirus salmonis can parasitize other salmonids to varying degrees, including brown trout (sea trout: Salmo trutta), charr (Salvelinus alpinus), and all species of Pacific salmon. In the case of Pacific salmon, coho, chum, and pink salmon (O. kisutch, O. keta, and O. gorbuscha, respectively) mount strong tissue responses to attaching L. salmonis, which lead to rejection within the first week of infection[4]. Pacific L. salmonis can also develop, but not complete, its full life cycle on the three-spined stickleback (Gasterosteus aculeatus L.)[5]. This has not been observed with Atlantic L. salmonis.

How planktonic stages of sea lice disperse and find new hosts is still not completely known. Temperature, light and currents are major factors and survival depends on salinity above 25 ppt[6][7][8][9]. It has been hypothesized that L. salmonis copepodids migrating upwards towards light and salmon smolt moving downwards at daybreak facilitate in finding a host[10]. Several field and modeling studies have examined copepodid populations in intertidal zones and have shown that planktonic stages can be transported tens of kilometres from their source[8][11][12].

The source of sea lice infections when salmon return from freshwater has always been a mystery. Sea lice die and fall off anadromous fish such as salmonids when they return to freshwater. Salmon go upstream in the fall to reproduce and the smolt do not return to saltwater until the next spring. It is possible that sea lice survive on fish that remain in the estuaries or that they transfer to an as yet unknown alternate host to spend the winter. Nonetheless, smolt get infected with sea lice larvae, or even possibly adults, when they enter the estuaries in the spring. It is also not known how sea lice distribute between fish in the wild. Adult stages of Lepeophtheirus spp. can transfer under laboratory conditions, but the frequency is low. Caligus spp. transfer quite readily and between different species of fish.


Morphology

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Lepeophtheirus salmonis tends to be approximately twice the size of most Caligus spp. The body consists of 4 regions: cephalothorax, fourth leg-bearing segment, genital complex, and abdomen[13]. The cephalothorax forms a broad shield that includes all of the body segments up to the third-leg bearing segment. It acts like a suction cup in holding the louse on the fish. All species have mouth parts shaped as a siphon or oral cone (characteristic of the Siphonostomatoida). The second antennae and oral appendages are modified to assist in holding the parasite on the fish. The second antenna is also used by males to grasp the female during copulation[14]. The adult females are always much larger than males and develop a very large genital complex which in many species makes up the majority of the body mass. Two egg strings of 500 to 1000 eggs (L. salmonis) that darken with maturation are approximately the same length as the female’s body. One female can produce 6-11 pairs of egg strings in a lifetime of approximately 7 months[8][15][16].


Development

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Sea lice have both free swimming (planktonic) and parasitic life stages. All stages are separated by moults[17][18][19][20]). The development rate for L. salmonis from egg to adult varies from 17 to 72 days depending on temperature. The life cycle of L. salmonis is shown in the figure; the sketches of the stages are from Schram[19].

Eggs hatch into nauplius I which moult to a second naupliar stage; both naupliar stages are non-feeding, depending on yolk reserves for energy, and adapted for swimming. The copepodid stage is the infectious stage and it searches for an appropriate host, likely by chemo- and mechanosensory clues. Currents, salinity, light, and other factors also will assist copepodids in finding a host[8]. Preferred settlement on the fish occurs in areas with the least hydrodynamic disturbance, particularly the fins and other protected areas[7][21]. Copepodids once attached to a suitable host feed for a period of time prior to moulting to the chalimus I stage. Sea lice continue their development through 3 additional chalimus stages each separated by a moult. A characteristic feature of all 4 chalimus stages is that they are physically attached to the host by a structure referred to as the frontal filament. There are differences in the timing, method of production and the physical structure of the frontal filament between different species of sea lice. With exception of a short period during the moult, the pre-adult and adult stages are mobile on the fish and, in some cases, can move between host fish. Adult females, being larger, occupy relatively flat body surfaces on the posterior ventral and dorsal midlines and may actually out-compete pre-adults and males at these sites[22].


Feeding Habits

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The naupliar and copepodid stages until they locate a host are non-feeding and live on endogenous food stores. Once attached to the host the copepodid stage begins feeding and begins to develop into the first chalimus stage. Copepods and chalimus stages have a developed gastrointestinal tract and feed on host mucus and tissues within range of their attachment. Pre-adult and adult sea lice, especially gravid females, are aggressive feeders, in some cases feeding on blood in addition to tissue and mucus. Blood is often seen in the digestive tract, especially of adult females. It is not known whether sea lice are vectors of disease, but they can be carriers of bacteria and viruses likely obtained from their attachment to and feeding on tissues of contaminated fish[23].


Disease

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Pathology

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Gravid female Lepeophtheiris salmonis on Atlantic salmon, Salmo salar

Sea lice cause physical and enzymatic damage at their sites of attachment and feeding which results in abrasion-like lesions that vary in their nature and severity depending upon a number of factors. These include host species, age and general health of the fish. It is not clear whether stressed fish are particularly prone to infestation. Sea lice infection itself causes a generalized chronic stress response in fish since feeding and attachment cause changes in the mucus consistency and damage the epithelium resulting in loss of blood and fluids, electrolyte changes, and cortisol release. This can decrease salmon immune responses and make them susceptible to other diseases and reduces growth and performance.

The degree of damage is also dependent on the species of sea lice, the developmental stages that are present, and the number of sea lice on a fish. There is little evidence of host tissue responses in Atlantic salmon at the sites of feeding and attachment, regardless of the development stage. In contrast, coho and pink salmon show strong tissue responses to L. salmonis characterized by epithelial hyperplasia and inflammation. This results in rejection of the parasite within the first week of infection in these species of salmonids[4]. Heavy infections of farmed Atlantic salmon and wild sockeye salmon (Oncorhynchus nerka) by L. salmonis can lead to deep lesions, particularly on the head region, even exposing the skull. With respect to farmed fish, disease of this magnitude is rarely seen anymore because of good therapeutic control measures being in place.


Wild - farmed fish interactions

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There is reported concern that sea lice flourishing on salmon farms can spread to nearby wild juvenile salmon and devastate these populations.[24] Sea trout populations in recent years have seriously declined due to infestation by sea lice from salmon farms.[25] Several scientific studies have suggested that caged farmed salmon harbour lice to a degree that can destroy surrounding wild salmon populations[26]. Other studies have shown that lice from farmed fish have relatively no effect on wild fish if good husbandry and adequate control measures are carried out[27] (also, see section: Control on salmon farms). Further studies to establish wild-farmed fish interactions are on-going, particularly in Canada, Scotland, Ireland, and Norway. A reference manual with protocol and guidelines for studying wild/cultured fish interactions with sea lice has been published[28].


Fish Farming

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Control on salmon farms

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This has been reviewed by Pike & Wadsworth[20], McVicar[29], and Costello[8]. Integrated pest management programs for sea lice are instituted or recommended in a number of countries, including Canada[30][31], Norway[27], Scotland[32], and Ireland[33]. Identification of epidemiological factors as potential risk factors for sea lice abundance[34] with effective sea lice monitoring programs have been shown to effectively reduce sea lice levels on salmon farms[35].


Natural predators

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Cleaner fish, including five species of wrasse (Labridae), are used on fish farms in Norway and to a lesser extent in Scotland, Shetland and Ireland[36]. Their potential has not been researched in other fish farming regions, such as Pacific and Atlantic Canada or Chile.


Husbandry

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Good husbandry techniques include fallowing, removal of dead and sick fish, prevention of net fouling, etc. Bay management plans are in place in most fish farming regions to keep sea lice below a level that could lead to health concerns on the farm or affect wild fish in surrounding waters. These include separation of year classes, counting and recording of sea lice on a prescribed basis, use of parasiticides when sea lice counts increase, and monitoring for resistance to parasiticides.


Salmon breeding

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Early findings suggested genetic variation in the susceptibility of Atlantic salmon to Caligus elongatus[37]. Research then began to identify trait markers[38] and recent studies have shown that susceptibility of Atlantic salmon to L. salmonis can be identified to specific families and that there is a link between MHC Class II and susceptibility to lice[39].


Drugs and Vaccines

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The range of therapeutants for farmed fish was limited, often due to regulatory processing limitations. All drugs used have been assessed for environmental impact and risks[40][41]. The parasiticides are classified into bath and in-feed treatments as follows:


Bath Treatments:

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There are both advantages and disadvantages to using bath treatments. Bath treatments are more difficult and need more manpower to administer, requiring skirts or tarpaulins to be placed around the cages to contain the drug. Prevention of reinfection is a challenge since it is practically impossible to treat an entire bay in a short time period. Since the volume of water is imprecise, the required concentration is not guaranteed. Crowding of fish to reduce the volume of drug can also stress the fish. Recent use of well-boats containing the drugs has reduced both the concentration and environmental concerns, although transferring fish to the well boat and back to the cage can be stressful. The major advantage to bath treatments is that all the fish will be treated equally, in contrast to in-feed treatments where amount of drug ingested can vary due to a number of reasons.

Organophosphates

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Organophosphates are acetylcholinesterase inhibitors and cause excitatory paralysis leading to death of sea lice when given as a bath treatment. Dichlorvos was used for many years in Europe and later replaced by azamethiphos, the active ingredient in Salmosan, which is safer for operators to handle[42]. Azamethiphos is water-soluble and broken down relatively quickly in the environment. Resistance to organophosphates began to develop in Norway in the mid 1990’s, apparently due to acetylcholinesterases being altered due to mutation[43]. Use has declined considerably with the introduction of SLICE, emamectin benzoate.

Pyrethroids

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Pyrethroids are direct stimulators of sodium channels in neuronal cells, inducing rapid depolarization and spastic paralysis leading to death. The effect is specific to the parasite since the drugs used are only slowly absorbed by the host and rapidly metabolized once absorbed. Cypermethrin (Excis, Betamax) and deltamethrin (Alphamax) are the two pyrethroids commonly used to control sea lice. Resistance to pyrethroids has been reported in Norway and appears to be due to a mutation leading to a structural change in the sodium channel which prevents pyrethroids from activating the channel[44]. Use of deltamethrin has been increasing as an alternate treatment with the rise in resistance observed with emamectin benzoate.

Topical Disinfectants

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Bathing fish with hydrogen peroxide (350-500 mg/L for 20 min) will remove mobile sea lice from fish. It is environmentally friendly since H2O2 dissociates to water and oxygen, but can be toxic to fish, depending on water temperature, as well as to operators[45]. It appears to knock the sea lice off the fish, leaving them capable of reattaching to other fish and reinitiating an infection.


In-feed Treatments:

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In-feed treatments are easier to administer and pose less environmental risk than bath treatments. Feed is usually coated with the drug and drug distribution to the parasite is dependent on the pharmacokinetics of the drug getting in sufficient quantity to the parasite. The drugs have high selective toxicity for the parasite, are quite lipid soluble so that there is sufficient drug to act for approximately 2 months, and any unmetabolized drug is excreted so slowly that there are little to no environmental concerns.

Avermectins

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Avermectins belong to the family of macrocyclic lactones and are the major drugs used as in-feed treatments to kill sea lice. The first avermectin used was ivermectinat doses close to the therapeutic level and was not submitted for legal approval for use on fish by its manufacturer. Ivermectin was toxic to some fish, causing sedation and central nervous system depression due to the drug’s ability to cross the blood-brain barrier. Emamectin benzoate, which is the active agent in the formulation SLICE[46], has been used since 1999 and has a greater safety margin on fish. It is administered at 50µg/kg/day for 7 days and is effective for two months, killing both chalimus and mobile stages. Withdrawal times vary with jurisdiction from zero in Canada to 175 degree days in Norway. Avermectins act by opening glutamate-gated chloride channels in arthropod neuromuscular tissues, causing hyperpolarization and flaccid paralysis leading to death. Resistance has been noted in Chalimus rogercresseyi in Chile and L. salmonis on North Atlantic fish farms. The resistance to likely due to prolonged use of the drug leading to up-regulation of P-glycoprotein[47], similar to what has been seen in nematode resistance to macrocyclic lactones[48].

Growth Regulators

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Teflubenzuron, the active agent in the formulation Calicide[49], is a chitin synthesis inhibitor and prevents moulting. It thus prevents further development of larval stages of sea lice, but has no effect on adults. It has been used only sparingly in sea lice control, largely due to concerns that it may affect the moult cycle of non-target crustaceans, although this has not been shown at the concentrations recommended[40].


Vaccines:

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A number of studies are underway to examine various antigens, particularly from the gastrointestinal tract and reproductive endocrine pathways, as vaccine targets, but no vaccine against sea lice has been reported to date.


Other points of interest:

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Branchiurans, family Argulidae, order Arguloida are known as fish lice and parasitize fish in freshwater.


References

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  1. ^ Walter, T.C., Boxshall, G. (Eds) 2009. “World Copepoda database”. Accessed through the World Register of Marine Species. http://www.marinespecies.org/aphia.php?p=taxdetails&id=349547 on 2009-08-17
  2. ^ Bravo, S. 2003. “Sea lice in Chilean salmon farms”. Bull. Eur. Ass. Fish Pathol. 23, 197–200.
  3. ^ Yazawa, R., Yasuike, M., Leong, J., von Schalburg, K.R., Cooper, G.A., Beetz-Sargent, M., Robb, A., Davidson, W.S., Jones, S.R., and Koop, B.F. 2008. “EST and mitochondrial DNA sequences support a distinct Pacific form of salmon louse, Lepeophtheirus salmonis”. Mar. Biotechnol. (NY) 10, 741-749. doi:10.1007/s10126-008-9112-y pmid:18574633
  4. ^ a b Wagner, G.N., Fast, M.D. & Johnson, S.C. 2008. “Physiology and immunology of Lepeophtheirus salmonis infections of salmonids”. Trends Parasitol. 24, 176-183
  5. ^ Jones, S.R.M., Prosperi-Porta, G., Kim, E., Callow, P., & Hargreaves, N.B. 2006. "The occurrence of Lepeophtheirus salmonis and Caligus clemensi (Copepoda: Caligidae) on threespine stickleback Gasterosteus aculeatus in coastal British Columbia". J. Parasitol. 92, 473-480.
  6. ^ Costelloe, M., Costelloe, J., O’Donohoe, G. Coghlan, N.J., Oonk, M., & van der Heijden, Y. 1998. “Planktonic distribution of sea lice larvae Lepeophtheirus salmonis, in Killary harbor, west coast of Ireland. J. Mar. Biol. Assoc. UK 78, 853-874
  7. ^ a b Genna, R.L., Mordue, W., Pike, A.W., & Mordue-Luntz, A.J. 2005. “Light intensity, salinity, and host velocity influence presettlement intensity and distribution on hosts by copepodids of sea lice, Lepeophtheirus salmonis”. Can. J. Fish. Aquat. Sci. 62, 2675-2682
  8. ^ a b c d e Costello, M.J. 2006. “Ecology of sea lice parasitic on farmed and wild fish”. Trends Parasitol. 22, 475-483
  9. ^ Brooks, K.M. 2005. “The effects of water temperature, salinity, and currents on the survival and distribution of the infective copepodid stage of sea lice (Lepeophtheirus salmonis) originating on Atlantic salmon farms in the Broughton Archipelago of British Columbia, Canada”. Rev. Fish. Sci. 13, 177-204
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  12. ^ Costello, M.J. 2009. "How sea lice from salmon farms may cause wild salmonid declines in Europe and North America and be a threat to fishes elsewhere." Proc. R. Soc. B. 276, 3385-3394 doi: 10:1098/rsbp.2009.0771
  13. ^ Johnson, S.C. & Albright, L.J. 1991. “The developmental stages of Lepeophtheirus salmonis (Krøyer, 1837) (Copepoda: Caligidae)”. Can. J. Zool. 69, 929-950
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  23. ^ Nylund, A., Bjørknes, B., & Wallace, C. 1991. “Lepeophtheirus salmonis – a possible vector in the spread of diseases on salmonids. Bull. Europ. Assoc. Fish Pathol. 11, 213-216
  24. ^ Martin Krkosek, Jennifer S. Ford, Alexandra Morton, Subhash Lele, Ransom A. Myers, and Mark A. Lewis (14 December 2007), "Declining Wild Salmon Populations in Relation to Parasites from Farm Salmon". Science 318 (5857), 1772.
  25. ^ Clover, Charles (2004). The End of the Line: How Overfishing is Changing the World and What We Eat. London: Ebury Press. ISBN 0-09-189780-7.
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  27. ^ a b Heuch, P.A. Bjørn, P.A., Finstad, B., Holst, J.C., Asplin, L. & Nilsen, F. 2005. “A review of The Norwegian Action Plan Against Salmon Lice on Salmonids: the effect on wild salmonids”. Aquaculture 246, 79-92
  28. ^ http://www.pacificsalmonforum.ca/wild/index.php, on 2009-09-17
  29. ^ McVicar, A.H. 2004. “Management actions in relation to the controversay about salmon lice infections in fish farms as a hazard to wild salmonid populations”. Aquacult. Res. 35, 751-758
  30. ^ http://www.hc-sc.gc.ca/cps-spc/pubs/pest/_fact-fiche/lice-pou/index-eng.php on 2009-09-11
  31. ^ http://www.al.gov.bc.ca/ahc/fish_health/SL%20Mgmnt%20Strat%202007%202008%20Final.pdf on 2009-09-11
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  33. ^ Grist B. 2002. “The regulatory system for aquaculture in the Republic of Ireland”. Pest Manag. Sci. 58: 609–615
  34. ^ Revie, C.W., Getinby, G., Treasurer, J.W. & Wallace, C. 2003. "Identifying epidemiological factors affecting sea lice Lepeophtheirus salmonis abundance on Scottish salmon farms using general linear models". Dis. Aquat. Org. 57, 85-95
  35. ^ Saksida, S., Karreman, G.A., Constantine, J., & Donald, A. 2007. "Differences in Lepeophtheirus salmonis abundance levels on Atlantic salmon farms in the Broughton Archipelago, British Columbia, Canada". J. Fish Dis. 30, 357-366
  36. ^ Treasurer, J.W. 2002. “A review of potential pathogens of sea lice and the application of cleaner fish in biological control”. Pest Manag. Sci. 58, 546-558
  37. ^ Mustafa, A. & MacKinnon, B.M. 1999. "Genetic variation in susceptibility of Atlantic salmon to the sea louse Caligus elongatus Nordmann 1882". Can. J. Zool. 77, 1332-1335
  38. ^ Jones, C.S., Lockyer, A.E., Verspoor, E., Secombes, C.J., & Noble, L.R. 2002. “Towards selective breeding of Atlantic salmon for sea louse resistance: approaches to identify trait markers”. Pest Manag. Sci. 58, 559-568
  39. ^ Glover, K.A., Grimholt, U., Bakke, H.G., Nilsen, F., Storset, A. &, Skaala, Ø. 2007. “Major histocompatibility complex (MHC) variation and susceptibility to the sea louse Lepeophtheirus salmonis in Atlantic salmon Salmo salar”. Dis. Aquat. Org. 76, 57-66
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  42. ^ Denholm, I., Devine, G.J., Horsberg, T.E., Sevatdal, S., Fallang, A., Nolan, D.V., & Powell, R. 2002. “Analysis and management of resistance to chemotherapeutants in salmon lice Lepeophtheirus salmonis (Copepoda: Caligidae)”. Pest Manag. Sci. 58, 528-536
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  44. ^ Fallang, A., Denholm, I., Horsberg, T.E., & Williamson, M.S. 2005. “Novel point mutation in the sodium channel gene of pyrethroid-resistant sea lice Lepeophtheirus salmonis (Crustacea: Copepoda)”. Dis. Aquat. Org. 65, 129-136
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  49. ^ EMEA 1999. “Teflubenzuron, summary report” http://www.emea.europa.eu/pdfs/vet/mrls/054799en.pdf on 2009-09-11


Category:Fish diseases Category:Veterinary parasitology Category:Aquaculture Category:Crustacean

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