User:Pacheco173ayy/Cleavage and polyadenylation specificity factor

Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. It is the first protein to bind to the signaling region near the cleavage site of the pre-mRNA, to which the poly(A) tail will be added by polynucleotide adenylyltransferase. The 10-30 nucleotide upstream signaling region of the cleavage site, polyadenylation signal (PAS), has the canonical nucleotide sequence AAUAAA, which is highly conserved across the vast majority of pre-mRNAs. A second downstream signaling region, located on the portion of the pre-mRNA that is cleaved before polyadenylation, consists of a GU-rich region required for efficient processing. The mature RNA are transported into the cytoplasm, where they are translated into proteins.^[1]

Structure

In mammals, CPSF has six subunits: CPSF-73, CPSF-100, CPSF-30, CPSF-160, WDR33, and Fip1.

Cleavage stimulation factor (CstF) has three subunits. CstF recognizes the second downstream signaling region of the cleavage site, which is a GU-rich sequence motif.

CPSF is a protein complex, consisting of four proteins: CPSF-73, CPSF-100, CPSF-30 and CPSF-160.

CPSF-73 is a zinc-dependent hydrolase which cleaves the mRNA precursor just downstream the polyadenylation signal sequence AAUAAA.^[2]

CPSF-160 is the largest subunit of CPSF and directly binds to the AAUAAA polyadenylation signal.^[3]

WDR33 and CPSF-30 recognize the polyadenylation signal (PAS) in pre-mRNA, which aids in defining the position of RNA cleavage. ^[1]

CPSF recruits proteins to the 3' region. Identified proteins that are coordinated by CPSF activity include: cleavage stimulatory factor and the two poorly understood cleavage factors. The binding of the polynucleotide adenylyltransferase responsible for actually synthesizing the tail is a necessary prerequisite for cleavage, thus ensuring that cleavage and polyadenylation are tightly coupled processes.

Genes

CPSF1, CPSF2, CPSF3, CPSF4, CPSF6

References

^ ^a ^b Sun, Yadong; Zhang, Yixiao; Hamilton, Keith; Manley, James L.; Shi, Yongsheng; Walz, Thomas; Tong, Liang (2018-02-13). "Molecular basis for the recognition of the human AAUAAA polyadenylation signal". Proceedings of the National Academy of Sciences. 115 (7). doi:10.1073/pnas.1718723115. ISSN 0027-8424. PMC 5816196. PMID 29208711.{{cite journal}}: CS1 maint: PMC format (link)
^ Mandel, Corey R.; Kaneko, Syuzo; Zhang, Hailong; Gebauer, Damara; Vethantham, Vasupradha; Manley, James L.; Tong, Liang (26 November 2006). "Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease". Nature. 444 (7121): 953–956. Bibcode:2006Natur.444..953M. doi:10.1038/nature05363. PMC 3866582. PMID 17128255.
^ Murthy, Kanneganti G K; Manley, James L (1 November 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes & Development. 9 (21): 2672–2683. doi:10.1101/gad.9.21.2672. PMID 7590244. Retrieved 17 December 2014.

Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott MP, Zipursky SL, Darnell J. (2004). Molecular Cell Biology. WH Freeman: New York, NY. 5th ed.
Murthy, KG; Manley, JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–2683. doi:10.1101/gad.9.21.2672. PMID 7590244.

External links

Cleavage+And+Polyadenylation+Specificity+Factor at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This is the sandbox page where you will draft your initial Wikipedia contribution.

If you're starting a new article, you can develop it here until it's ready to go live.

If you're working on improvements to an existing article, copy only one section at a time of the article to this sandbox to work on, and be sure to use an edit summary linking to the article you copied from. Do not copy over the entire article. You can find additional instructions here.

Remember to save your work regularly using the "Publish page" button. (It just means 'save'; it will still be in the sandbox.) You can add bold formatting to your additions to differentiate them from existing content.

Bibliography sandbox

Article Draft

Lead

Article body

References

[:0-1] Sun, Yadong; Zhang, Yixiao; Hamilton, Keith; Manley, James L.; Shi, Yongsheng; Walz, Thomas; Tong, Liang (2018-02-13). "Molecular basis for the recognition of the human AAUAAA polyadenylation signal". Proceedings of the National Academy of Sciences. 115 (7). doi:10.1073/pnas.1718723115. ISSN 0027-8424. PMC 5816196. PMID 29208711.{{cite journal}}: CS1 maint: PMC format (link)

[mandel2006-2] Mandel, Corey R.; Kaneko, Syuzo; Zhang, Hailong; Gebauer, Damara; Vethantham, Vasupradha; Manley, James L.; Tong, Liang (26 November 2006). "Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease". Nature. 444 (7121): 953–956. Bibcode:2006Natur.444..953M. doi:10.1038/nature05363. PMC 3866582. PMID 17128255.

[murthy1995-3] Murthy, Kanneganti G K; Manley, James L (1 November 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes & Development. 9 (21): 2672–2683. doi:10.1101/gad.9.21.2672. PMID 7590244. Retrieved 17 December 2014.

[1]

[2]

[3]