User:Msmrugby/sandbox/Mike's Sandbox

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Summary of characteristics of target article^[2]

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Week 5 Assignment

There is a new purification method for recombinant proteins that has been developed that is able to remove an affinity tag by inducing auto-cleavage.^[3] The new method utilizes the multiple domains of Ssp DnaB split Intein, and avoids the issue of premature cleaving of the proteins that have been tagged and expressed.^[3] According to a 2013 Shah article, "Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans."^[4]

References

^ [| WikiPedia Five Pillars]
^ [| Article Assessment]
^ ^a ^b Lu, Wei; Sun, Ziyong; Tang, Yanchun; Chen, Junyong; Tang, Fengyuan; Zhang, Jing; Liu, Jian-Ning (6 May 2011). "Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage". Journal of Chromatography. 1218 (18): 2553–2560. doi:10.1016/j.chroma.2011.02.053. PMID 21397239. Retrieved 25 February 2014.
^ Shah, Neel H.; Eryilmaz, Ertan; Cowburn, David; Muir, Tom W. (2013 Dec 11). "Naturally split inteins assemble through a "capture and collapse" mechanism". Journal of the American Chemical Society. 135 (49): 18673–81. doi:10.1021/ja4104364. PMC 3865799. PMID 24236406. {{cite journal}}: Check date values in: |date= (help)

[1] [| WikiPedia Five Pillars]

[2] [| Article Assessment]

[Lu-3] Lu, Wei; Sun, Ziyong; Tang, Yanchun; Chen, Junyong; Tang, Fengyuan; Zhang, Jing; Liu, Jian-Ning (6 May 2011). "Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage". Journal of Chromatography. 1218 (18): 2553–2560. doi:10.1016/j.chroma.2011.02.053. PMID 21397239. Retrieved 25 February 2014.

[Shah-4] Shah, Neel H.; Eryilmaz, Ertan; Cowburn, David; Muir, Tom W. (2013 Dec 11). "Naturally split inteins assemble through a "capture and collapse" mechanism". Journal of the American Chemical Society. 135 (49): 18673–81. doi:10.1021/ja4104364. PMC 3865799. PMID 24236406. {{cite journal}}: Check date values in: |date= (help)

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