User:Kand453/Lysis

This is the sandbox page where you will draft your initial Wikipedia contribution.

If you're starting a new article, you can develop it here until it's ready to go live.

If you're working on improvements to an existing article, copy only one section at a time of the article to this sandbox to work on, and be sure to use an edit summary linking to the article you copied from. Do not copy over the entire article. You can find additional instructions here.

Remember to save your work regularly using the "Publish page" button. (It just means 'save'; it will still be in the sandbox.) You can add bold formatting to your additions to differentiate them from existing content.

Bibliography sandbox

Article Draft-adding to chemical lysis section

Chemical lysis

This method uses chemical disruption. It is the most popular and simple approach. Chemical lysis chemically deteriorates/solubilizes the proteins and lipids present within the membrane of targeted cells. Common lysis buffers contain sodium hydroxide (NaOH) and sodium dodecyl sulfate (SDS). Cell lysis is best done at a pH range of 11.5-12.5. Although simple, it is a slow process, taking anywhere from 6 to 12 hours^[1].

References

^ Shehadul Islam, Mohammed; Aryasomayajula, Aditya; Selvaganapathy, Ponnambalam Ravi (2017-03). "A Review on Macroscale and Microscale Cell Lysis Methods". Micromachines. 8 (3): 83. doi:10.3390/mi8030083. ISSN 2072-666X. PMC 6190294. {{cite journal}}: Check date values in: |date= (help)CS1 maint: PMC format (link) CS1 maint: unflagged free DOI (link)

[1] Shehadul Islam, Mohammed; Aryasomayajula, Aditya; Selvaganapathy, Ponnambalam Ravi (2017-03). "A Review on Macroscale and Microscale Cell Lysis Methods". Micromachines. 8 (3): 83. doi:10.3390/mi8030083. ISSN 2072-666X. PMC 6190294. {{cite journal}}: Check date values in: |date= (help)CS1 maint: PMC format (link) CS1 maint: unflagged free DOI (link)

[1]