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User:JustinCHEM446/Outline

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Protein Adsorption

Trevor Shepherd, Justin Hui, Jessica Cho

Methods to investigate protein adsorption

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1. Fluorescent labeling / Radio labeling

2. Atomic Force Microscopy (AFM)

Measures the force of adhesion between a protein-coated probe and the arrayed polymers.

3. Ceramics: 2D PAGE

4. Surface-Matrix Assisted Laser Desorption Ionization Mass Spectrometry (Surface-MALDIMS)[1]

Instrument uses a pulsed UV laser for protein ionization and delayed extraction combined with time-of-flight analysis for mass separation

Metals

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Relevance

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1. Sterilization of implants and medical devices (reduced risk of disease transmission

a. Surgical tool design

2. Reduced risk of corrosion

3. Engineer devices to have a lower risk of rejection after implantation

a. Incorporating native proteins will reduce the host immune and inflammatory response

Chemical composition

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1. Crystalline structure

a. Crystal packing exposes potential metal-ion-dependent adhesion site (MIDAS) on one edge of the beta-sandwich and may serve as a binding site for collagens and other ligands. [2]

Coordination number

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IMFs

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Polymers

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Chemical composition

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Coordination number

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IMFs

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1. Dispersion

2. Dipole-dipole

3. Hydrogen Bonding

Ceramics

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Chemical composition

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Coordination number

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IMFs

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Effect of surface properties on adsorption

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Surface wettability

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1. Contact angle

2. Surface energy

pH

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References

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