User:Elleevikram

Feedback on your article (N-Acetylglutamic acid) by Kensei Kishimoto:

##Thoughts

You added a lot of helpful and detailed information in the article. I now know that NAG is involved in metabolic pathways both in prokaryotes and eukaryotes.

You should be consistent with your use of abbreviations. You can use the full chemical name or abbreviated NAG.

You can also expand See Also category since it is involved in so many different things like urea cycle or NAG synthase.

I wasn't sure about this sentence and what it meant: "NAGS synthesizes NAG by using acetyl-coenzyme A to acetylate glutamate." Does it make acetylate glutamate?

I don't see any bias and the article flows well too.

##Minor suggestions

Your overall structure of the article is organized and logical. You have chemical information on the right that is useful for further look-ups. I feel like you can put your spectra there rather than dedicating a section for two NMR spetctra.

I am worried that this may be a bit detailed especially in Biosynthesis since it does talk about a lot of new concepts and new metabolic cycles. But at the same time, they are relevant to the NGS. So if there is a way to simplify some of them even a bit, that will help with reader retention and easier communication.

I am not sure if you need to have references for each sentence, but having that might give your article more credibility.

Kensei Kishimoto (talk) 06:10, 31 May 2018 (UTC)

N-Acetylglutamic Acid Peer Review -- Marie Bae

Overall, great work! I loved how you transformed a short stub/start article, into an interesting, comprehensive summary about N-acetylglutamic acid (NAG)! I thought your article already is very organized and flows pretty well, but I do think that the NMR spectra could maybe be more relevant at a different section (see Discovery and Biosynthesis). The content in each section does seem appropriate for this article. I don't think there was excessive or redundant information, as each paragraph helped me learn more about NAG itself. I did not detect any bias or opinions either. Great work!

• Intro:
  • I really liked your intro, which offers some good background about the main highlights of your topic. The lead paragraph definitely explained what NAG in a concise, organized manner. I would suggest explaining that NAG is the first intermediate of arginine biosynthesis and cofactor in the urea cycle in the first sentence (basically moving the last sentence to the first sentence) for better flow, as I think the last sentence encompasses what NAG is best and you can expand from that by describing how its made.
• Discovery and Biosynthesis
  • This is more of a structural suggestion but here it goes: I was thinking maybe you can split Discovery and Biosynthesis into two sections (split first paragraph into Discovery and second-fourth paragraphs into Biosynthesis).
    • Discovery (and Structure?): I thought here would be a great place to add your NMR spectra. You could even probably go into how the data determined the structure (maybe in a caption), and go into the structural components (such as functional groups) of your molecule.
    • Biosynthesis:
      • Great info for Biosynthesis! It was structured really well, delving into the different biosynthesis methods in rhizobium, prokaryotes in one paragraph and eukaryotes in the other.
      • One minor edit I would suggest is changing "method of choice" to "preferred method" just to be concise, but this is just more of a personal preference.
• Biological Roles
  • Vertebrates and Mammals
    • I would suggest adding an image of the urea cycle here to help the reader imagine the pathway a little better. Maybe you can also show in the diagram where NAG is in the cycle?
    • Minor grammar edits I made to this section: when NAG "is not present" and "56%.,"
    • Is there a cofactor associated with cytoplasmic carbamyl phosphate synthetase? Maybe mention that "in contrast, ___________ is the ______________ to..." and add a link to that cofactor? OR: I think it helped me reaffirm that NAG is only involved in mitochondrial catalysis, so maybe emphasize that part (the last sentence in the first paragraph) at the beginning instead of at the end?
    • Question: What is the name of the NAGS coding gene? Maybe you can give the name for the NAGS coding gene (and maybe link it to another wiki article)?
  • Bacteria
    • Simple and concise, but still very informative!
  • White Clover Seedling Roots
    • Very interesting information!

• References
  • Content is properly cited, and there are enough references.
  • Based on the titles and that most of the sources have DOIs or PMIDs (the three that do not are journal articles still seem highly relevant (one from a chemistry database, two for NMR spectra), I thought your sources were reliable and relevant. Don't forget to cite your last paragraph in the Vertebrates and Mammals section!

Sincerely,

Marie Bae

P.S.: I think you were the reviewer who gave me some awesome feedback about my article, so thanks so much for all your suggestions!