Tight junction protein ZO-1

TJP1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2H2B, 2H2C, 2H3M, 2JWE, 2KXR, 2KXS, 2RCZ, 3CYY, 3LH5, 3SHU, 3SHW, 3TSV, 3TSW, 3TSZ, 4OEO, 4OEP, 4Q2Q, 4YYX
Identifiers
Aliases	TJP1, ZO-1, Tight junction protein 1
External IDs	OMIM: 601009; MGI: 98759; HomoloGene: 2445; GeneCards: TJP1; OMA:TJP1 - orthologs
Gene location (Human)
Chr.	Chromosome 15 (human)
End	29,968,915 bp
Gene location (Mouse)
Chr.	Chromosome 7 (mouse)
End	65,177,529 bp
RNA expression pattern
	Top expressed in
	corpus callosum; ; sural nerve; ; placenta; ; tibial arteries; ; right lung; ; smooth muscle tissue; ; Achilles tendon; ; endometrium; ; epithelium of colon; ; C1 segment;
	Top expressed in
	left lung lobe; ; vestibular sensory epithelium; ; gastrula; ; vestibular membrane of cochlear duct; ; utricle; ; stria vascularis; ; external carotid artery; ; cumulus cell; ; epithelium of lens; ; iris;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	calmodulin binding; protein binding; cadherin binding; protein domain specific binding; connexin binding; protein C-terminus binding;
Cellular component	membrane; bicellular tight junction; plasma membrane; basolateral plasma membrane; gap junction; apical junction complex; cytosol; cytoplasm; apical part of cell; cell junction; podosome; cell projection; nucleus; cell-cell junction; adherens junction; intercalated disc; apical plasma membrane; apicolateral plasma membrane; intercellular canaliculus; tight junctions; protein-containing complex;
Biological process	establishment of endothelial intestinal barrier; hippo signaling; cell-cell junction assembly; regulation of bicellular tight junction assembly; blastocyst formation; hearing; response to lipopolysaccharide; negative regulation of vascular permeability; response to ethanol; response to magnetism; cellular response to glucose stimulus;
	Sources:Amigo / QuickGO
Orthologs
	7082
	21872
	ENSG00000277401; ENSG00000104067
	ENSMUSG00000030516
	Q07157
	P39447
NM_001301025; NM_001301026; NM_003257; NM_175610; NM_001330239;
	NM_001355012; NM_001355013; NM_001355014; NM_001355015
	NM_001163574; NM_009386
NP_001287954; NP_001287955; NP_001317168; NP_003248; NP_783297;
	NP_001341941; NP_001341942; NP_001341943; NP_001341944; NP_001287954.1; NP_001287955.1
	NP_001157046; NP_033412
	Wikidata
View/Edit Human	View/Edit Mouse

Tight junction protein ZO-1 also known as Zonula Occludens-1 (ZO-1), is a 220-kD peripheral membrane protein that is encoded by the TJP1 gene in humans.^[5] It belongs to the family of zonula occludens proteins (ZO-1, ZO-2, and ZO-3), which are tight junction-associated proteins and of which, ZO-1 is the first to be cloned. It was first isolated in 1986 by Stevenson and Goodenough using a monoclonal antibody raised in rodent liver to recognise a 225-kD polypeptide in whole liver homogenates and in tight junction-enriched membrane fractions.^[6] It has a role as a scaffold protein which cross-links and anchors Tight Junction (TJ) strand proteins, which are fibril-like structures within the lipid bilayer, to the actin cytoskeleton.^[7]

Function

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.^[8]

Interactions

Tight junction protein 1 has been shown to interact with:

F11 receptor,^[9]^[10]
GJA3,^[11]
GJA8,^[11]
Gap junction protein, alpha 1,^[12]
KIRREL,^[13]^[14]
MLLT4,^[15]
Occludin,^[16]^[17]
TJP3,^[18] and
Tight junction protein 2.^[16]^[18]^[19]

References

^ ^a ^b ^c ENSG00000104067 GRCh38: Ensembl release 89: ENSG00000277401, ENSG00000104067 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000030516 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Mohandas TK, Chen XN, Rowe LB, Birkenmeier EH, Fanning AS, Anderson JM, Korenberg JR (December 1995). "Localization of the tight junction protein gene TJP1 to human chromosome 15q13, distal to the Prader-Willi/Angelman region, and to mouse chromosome 7". Genomics. 30 (3): 594–7. doi:10.1006/geno.1995.1281. PMID 8825647.
^ Stevenson BR, Siliciano JD, Mooseker MS, Goodenough DA (September 1986). "Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia". The Journal of Cell Biology. 103 (3): 755–66. doi:10.1083/jcb.103.3.755. PMC 2114282. PMID 3528172.
^ Itoh M, Nagafuchi A, Moroi S, Tsukita S (July 1997). "Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments". The Journal of Cell Biology. 138 (1): 181–92. doi:10.1083/jcb.138.1.181. PMC 2139940. PMID 9214391.
^ "Entrez Gene: TJP1 tight junction protein 1 (zona occludens 1)".
^ Ebnet K, Schulz CU, Meyer Zu Brickwedde MK, Pendl GG, Vestweber D (Sep 2000). "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1". The Journal of Biological Chemistry. 275 (36): 27979–88. doi:10.1074/jbc.M002363200. PMID 10856295.
^ Ebnet K, Aurrand-Lions M, Kuhn A, Kiefer F, Butz S, Zander K, Meyer zu Brickwedde MK, Suzuki A, Imhof BA, Vestweber D (Oct 2003). "The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity". Journal of Cell Science. 116 (Pt 19): 3879–91. doi:10.1242/jcs.00704. PMID 12953056.
^ ^a ^b Nielsen PA, Baruch A, Shestopalov VI, Giepmans BN, Dunia I, Benedetti EL, Kumar NM (Jun 2003). "Lens connexins alpha3Cx46 and alpha8Cx50 interact with zonula occludens protein-1 (ZO-1)". Molecular Biology of the Cell. 14 (6): 2470–81. doi:10.1091/mbc.E02-10-0637. PMC 194895. PMID 12808044.
^ Giepmans BN, Moolenaar WH (1998). "The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein". Current Biology. 8 (16): 931–4. doi:10.1016/S0960-9822(07)00375-2. PMID 9707407.
^ Liu G, Kaw B, Kurfis J, Rahmanuddin S, Kanwar YS, Chugh SS (Jul 2003). "Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability". The Journal of Clinical Investigation. 112 (2): 209–21. doi:10.1172/JCI18242. PMC 164293. PMID 12865409.
^ Huber TB, Schmidts M, Gerke P, Schermer B, Zahn A, Hartleben B, Sellin L, Walz G, Benzing T (Apr 2003). "The carboxyl terminus of Neph family members binds to the PDZ domain protein zonula occludens-1". The Journal of Biological Chemistry. 278 (15): 13417–21. doi:10.1074/jbc.C200678200. PMID 12578837.
^ Yamamoto T, Harada N, Kawano Y, Taya S, Kaibuchi K (May 1999). "In vivo interaction of AF-6 with activated Ras and ZO-1". Biochemical and Biophysical Research Communications. 259 (1): 103–7. doi:10.1006/bbrc.1999.0731. PMID 10334923.
^ ^a ^b Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM (Nov 1998). "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton". The Journal of Biological Chemistry. 273 (45): 29745–53. doi:10.1074/jbc.273.45.29745. PMID 9792688.
^ Rao RK, Basuroy S, Rao VU, Karnaky Jr KJ, Gupta A (Dec 2002). "Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-beta-catenin complexes from the cytoskeleton by oxidative stress". The Biochemical Journal. 368 (Pt 2): 471–81. doi:10.1042/BJ20011804. PMC 1222996. PMID 12169098.
^ ^a ^b Wittchen ES, Haskins J, Stevenson BR (Dec 1999). "Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3". The Journal of Biological Chemistry. 274 (49): 35179–85. doi:10.1074/jbc.274.49.35179. PMID 10575001.
^ Itoh M, Morita K, Tsukita S (Feb 1999). "Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin". The Journal of Biological Chemistry. 274 (9): 5981–6. doi:10.1074/jbc.274.9.5981. PMID 10026224.

External links

Overview of all the structural information available in the PDB for UniProt: Q07157 (Human Tight junction protein ZO-1) at the PDBe-KB.
Overview of all the structural information available in the PDB for UniProt: P39447 (Mouse Tight junction protein ZO-1) at the PDBe-KB.

[refGRCh38Ensembl-1] ENSG00000104067 GRCh38: Ensembl release 89: ENSG00000277401, ENSG00000104067 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000030516 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8825647-5] Mohandas TK, Chen XN, Rowe LB, Birkenmeier EH, Fanning AS, Anderson JM, Korenberg JR (December 1995). "Localization of the tight junction protein gene TJP1 to human chromosome 15q13, distal to the Prader-Willi/Angelman region, and to mouse chromosome 7". Genomics. 30 (3): 594–7. doi:10.1006/geno.1995.1281. PMID 8825647.

[6] Stevenson BR, Siliciano JD, Mooseker MS, Goodenough DA (September 1986). "Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia". The Journal of Cell Biology. 103 (3): 755–66. doi:10.1083/jcb.103.3.755. PMC 2114282. PMID 3528172.

[7] Itoh M, Nagafuchi A, Moroi S, Tsukita S (July 1997). "Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments". The Journal of Cell Biology. 138 (1): 181–92. doi:10.1083/jcb.138.1.181. PMC 2139940. PMID 9214391.

[8] "Entrez Gene: TJP1 tight junction protein 1 (zona occludens 1)".

[pmid10856295-9] Ebnet K, Schulz CU, Meyer Zu Brickwedde MK, Pendl GG, Vestweber D (Sep 2000). "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1". The Journal of Biological Chemistry. 275 (36): 27979–88. doi:10.1074/jbc.M002363200. PMID 10856295.

[pmid12953056-10] Ebnet K, Aurrand-Lions M, Kuhn A, Kiefer F, Butz S, Zander K, Meyer zu Brickwedde MK, Suzuki A, Imhof BA, Vestweber D (Oct 2003). "The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity". Journal of Cell Science. 116 (Pt 19): 3879–91. doi:10.1242/jcs.00704. PMID 12953056.

[pmid12808044-11] Nielsen PA, Baruch A, Shestopalov VI, Giepmans BN, Dunia I, Benedetti EL, Kumar NM (Jun 2003). "Lens connexins alpha3Cx46 and alpha8Cx50 interact with zonula occludens protein-1 (ZO-1)". Molecular Biology of the Cell. 14 (6): 2470–81. doi:10.1091/mbc.E02-10-0637. PMC 194895. PMID 12808044.

[pmid9707407-12] Giepmans BN, Moolenaar WH (1998). "The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein". Current Biology. 8 (16): 931–4. doi:10.1016/S0960-9822(07)00375-2. PMID 9707407.

[pmid12865409-13] Liu G, Kaw B, Kurfis J, Rahmanuddin S, Kanwar YS, Chugh SS (Jul 2003). "Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability". The Journal of Clinical Investigation. 112 (2): 209–21. doi:10.1172/JCI18242. PMC 164293. PMID 12865409.

[pmid12578837-14] Huber TB, Schmidts M, Gerke P, Schermer B, Zahn A, Hartleben B, Sellin L, Walz G, Benzing T (Apr 2003). "The carboxyl terminus of Neph family members binds to the PDZ domain protein zonula occludens-1". The Journal of Biological Chemistry. 278 (15): 13417–21. doi:10.1074/jbc.C200678200. PMID 12578837.

[pmid10334923-15] Yamamoto T, Harada N, Kawano Y, Taya S, Kaibuchi K (May 1999). "In vivo interaction of AF-6 with activated Ras and ZO-1". Biochemical and Biophysical Research Communications. 259 (1): 103–7. doi:10.1006/bbrc.1999.0731. PMID 10334923.

[pmid9792688-16] Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM (Nov 1998). "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton". The Journal of Biological Chemistry. 273 (45): 29745–53. doi:10.1074/jbc.273.45.29745. PMID 9792688.

[pmid12169098-17] Rao RK, Basuroy S, Rao VU, Karnaky Jr KJ, Gupta A (Dec 2002). "Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-beta-catenin complexes from the cytoskeleton by oxidative stress". The Biochemical Journal. 368 (Pt 2): 471–81. doi:10.1042/BJ20011804. PMC 1222996. PMID 12169098.

[pmid10575001-18] Wittchen ES, Haskins J, Stevenson BR (Dec 1999). "Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3". The Journal of Biological Chemistry. 274 (49): 35179–85. doi:10.1074/jbc.274.49.35179. PMID 10575001.

[pmid10026224-19] Itoh M, Morita K, Tsukita S (Feb 1999). "Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin". The Journal of Biological Chemistry. 274 (9): 5981–6. doi:10.1074/jbc.274.9.5981. PMID 10026224.

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Tight junction protein ZO-1

Function

Interactions

See also

References

Further reading

External links