Talk:Zbyszek Darzynkiewicz

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Research Highlights

"He authored or co-authored 15 books and holds 8 US patents. The most widely recognized findings and the methodologies developed in his laboratory are:

The flow cytometric approach to differentially stain RNA versus DNA with acridine orange.^[1]. This method was the first to identify non-cycling (quiescent; G0 cells) and recognize other compartments of the cell cycle, is still being used in cell biology.^[2]

Another widely used ^[3] methodology is (the TUNEL assay)^[4]^[5]

The TUNEL assay made it possible to detect DNA fragmentation in nuclei of sperm cells of men with decreased fertility^[6] This methodology, recognized by WHO, serves as the means of identification of sperm cells abnormality and detect infertility.^[7]

The Fluorochrome Labeled Inhibitors of Caspases (FLICA assay) which detects caspase activation during apoptosis in relation to the cell cycle phase,^[8] has found wide application in biology and medicine^[9]

Still another methodology that found numerous applications in cell biology and medicine^[10]to detect DNA denaturation made use of the metachromatic properties of acridine orangeto detect denaturation of DNA in individual cells.^[11].

The above methodology when applied to sperm cells detected abnormality of chromatin structure of these cells associated with infertility^[12]This assay, in conjunction with the TUNEL assay is used as the key method to assess male fertility in husbandry and human clinic^[13]

The author pioneered in developing flow cytometric approaches with phosphospecific antibodies to detect phosphorylation of nuclear proteins such as pRB, histone H3 (serine10)and histone H2AX (γH2AX) in relation to the cell cycle phase^[14]^[15]"

References

^ Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1976) Lymphocyte stimulation: A rapid multiparameter analysis. Proc Natl Acad Sci USA 73:2881-2884, PMID: 822422
^ http://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:IjCSPb-OGe4C
^ http://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:u-x6o8ySG0sC
^ Gorczyca W, Gong J, Darzynkiewicz. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945-1951 PMID: 8467513 Online ISSN 1538-7445.
^ Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, PMID: 1333943
^ Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. (1993) Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 207:202-205. PMID: 8391465 DOI: 10.1006/excr.1993.1182
^ http://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:4DMP91E08xMC
^ Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. (2000) Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 259: 308-313.
^ http://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&cstart=20&citation_for_view=olM19WIAAAAJ:aqlVkmm33-oC
^ http://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:4DMP91E08xMC
^ Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1975) Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 90:411-428, PMID: 46199
^ Evenson DP, Darzynkiewicz Z, Melamed MR. (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131-1133. PMID: 7444440
^ DP Evenson, LK Jost, D Marshall, MJ Zinaman, E Clegg, K Purvis, P De Angelis, OP Claussen. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction 19994;4/1/1039-1049 https://doi.org/10.1093/humrep/14.4.1039
^ http://www.google.com/patents/US7070943
^ http://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&cstart=60&sortby=pubdate&citation_for_view=olM19WIAAAAJ:j5aT6aphRxQC

[1] Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1976) Lymphocyte stimulation: A rapid multiparameter analysis. Proc Natl Acad Sci USA 73:2881-2884, PMID: 822422

[2] ttp://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:IjCSPb-OGe4C

[3] ttp://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:u-x6o8ySG0sC

[4] Gorczyca W, Gong J, Darzynkiewicz. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945-1951 PMID: 8467513 Online ISSN 1538-7445.

[5] Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, PMID: 1333943

[6] Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. (1993) Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 207:202-205. PMID: 8391465 DOI: 10.1006/excr.1993.1182

[7] ttp://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:4DMP91E08xMC

[8] Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. (2000) Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 259: 308-313.

[9] ttp://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&cstart=20&citation_for_view=olM19WIAAAAJ:aqlVkmm33-oC

[10] ttp://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&citation_for_view=olM19WIAAAAJ:4DMP91E08xMC

[11] Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1975) Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 90:411-428, PMID: 46199

[12] Evenson DP, Darzynkiewicz Z, Melamed MR. (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131-1133. PMID: 7444440

[13] DP Evenson, LK Jost, D Marshall, MJ Zinaman, E Clegg, K Purvis, P De Angelis, OP Claussen. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction 19994;4/1/1039-1049 https://doi.org/10.1093/humrep/14.4.1039

[14] ttp://www.google.com/patents/US7070943

[15] ttp://scholar.google.com/citations?view_op=view_citation&hl=en&user=olM19WIAAAAJ&cstart=60&sortby=pubdate&citation_for_view=olM19WIAAAAJ:j5aT6aphRxQC

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