Talk:Reporter gene

	This article is within the scope of WikiProject Molecular Biology, a collaborative effort to improve the coverage of Molecular Biology on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks.Molecular BiologyWikipedia:WikiProject Molecular BiologyTemplate:WikiProject Molecular BiologyMolecular Biology articles
???	This article has not yet received a rating on the importance scale.
	This article is supported by the Genetics task force (assessed as High-importance).
	This article is supported by the Molecular and Cell Biology task force (assessed as Mid-importance).

GFP Fluorescence Edit

I made an edit for scientific accuracy because green fluorescent protein (GFP) glows green under blue light as well as under ultraviolet light.

This is supported by the article that is cited which says "under illumination with the 360–400 nm spectrum of light that excites GFP fluorescence"^[1] which corresponds to ultraviolet light.^[2]

I don't believe that my edit would constitute a unit conversion—or at least, not one that is valid under WP:SYNTH—hence why I provided a citation. Overall, the citations for that line could be tidied up, but I think the best fix would be a single source that encompasses both the primary source and covers the range of light.

Kilvin the Futz-y Enterovirus (talk) 20:43, 18 March 2024 (UTC)[reply]

References

^ "Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells". The FASEB Journal. March 2005. doi:10.1096/fj.04-3180fje. ISSN 0892-6638. PMC 1242169. PMID 15640280.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ "Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222". Nature Structural Biology. Jan 2002. doi:10.1038/nsb739. ISSN 1072-8368. PMID 11740505.

Spamming Wikipedia

Biologicalworld.com has spammed wikipedia like no tomorrow. It is a site of only a few pages and a LOT of adsense. Not much information is given except for "protocols" which are not referenced, and cannot be trusted from a site of that quality.

check: Links from Wikipedia

The following have been cleaned up:

wiki.riteme.site/wiki/Plasmid
wiki.riteme.site/wiki/Gel_electrophoresis
wiki.riteme.site/wiki/Green_fluorescent_protein
wiki.riteme.site/wiki/Homology_(biology)
wiki.riteme.site/wiki/HeLa
wiki.riteme.site/wiki/Protease
wiki.riteme.site/wiki/Restriction_enzyme
wiki.riteme.site/wiki/Petri_dish
wiki.riteme.site/wiki/Structural_domain
wiki.riteme.site/wiki/Trypsin
wiki.riteme.site/wiki/Oligonucleotide
wiki.riteme.site/wiki/Transmission_electron_microscope
wiki.riteme.site/wiki/Agar_plate
wiki.riteme.site/wiki/Calcium_phosphate
wiki.riteme.site/wiki/Disulfide_bond
wiki.riteme.site/wiki/Denaturation_(biochemistry)
wiki.riteme.site/wiki/DNA_ligase
wiki.riteme.site/wiki/Wild_type
wiki.riteme.site/wiki/Tissue_culture
wiki.riteme.site/wiki/Transmission_electron_microscopy
wiki.riteme.site/wiki/Reporter_gene
wiki.riteme.site/wiki/Northern_blot
wiki.riteme.site/wiki/Protein_engineering
wiki.riteme.site/wiki/Sticky_end/blunt_end
wiki.riteme.site/wiki/Taq_polymerase
wiki.riteme.site/wiki/Protein_domain
wiki.riteme.site/wiki/Coomassie
wiki.riteme.site/wiki/Native_state
wiki.riteme.site/wiki/Chinese_Hamster_Ovary_cell
wiki.riteme.site/wiki/Peptidase
wiki.riteme.site/wiki/Visking_tubing
wiki.riteme.site/wiki/Streptavidin
wiki.riteme.site/wiki/Microtiter_plate
wiki.riteme.site/wiki/Subcloning
wiki.riteme.site/wiki/Ion_exchange_chromatography
wiki.riteme.site/wiki/Thermal_cycler
wiki.riteme.site/wiki/Bovine_serum_albumin
wiki.riteme.site/wiki/Phosphate_buffered_saline
wiki.riteme.site/wiki/Glutathione_S-transferase
wiki.riteme.site/wiki/HEPES
wiki.riteme.site/wiki/Ortholog
wiki.riteme.site/wiki/Proteases
wiki.riteme.site/wiki/Salting_out
wiki.riteme.site/wiki/Fetal_bovine_serum
wiki.riteme.site/wiki/Proteolytic_enzyme
wiki.riteme.site/wiki/DNA_end
wiki.riteme.site/wiki/Supernatant
wiki.riteme.site/wiki/ABTS
wiki.riteme.site/wiki/Conserved_sequence
wiki.riteme.site/wiki/Peptide_mass_fingerprinting
wiki.riteme.site/wiki/Dithiothreitol
wiki.riteme.site/wiki/Uranyl_acetate

and many more Sciencetalks (talk) 03:24, 4 January 2008 (UTC)[reply]

References

[1] "Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells". The FASEB Journal. March 2005. doi:10.1096/fj.04-3180fje. ISSN 0892-6638. PMC 1242169. PMID 15640280.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[2] "Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222". Nature Structural Biology. Jan 2002. doi:10.1038/nsb739. ISSN 1072-8368. PMID 11740505.

[1]

[2]