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For what it's worth, the following line got tagged with a citation request:
It is widely speculated among historians that Charles was Isabel's father; Germaine refers to her in her will as the "Infanta Isabel"—a title that only makes sense if her father was the King.[citation needed]
The source for this was the Spanish Wikipedia article; I translated some of the information there over here awhile back. Here's what it says:
Pronto surgió entre los dos una apasionada relación amorosa de la que nació una hija, Isabel, y aunque nunca fue reconocida oficialmente, Germana de Foix se refiere a ella en su testamento como la «infanta Isabel» –título que no le podía corresponder– y a su padre como «el emperador».
Of course, Wikipedia can't be used as a source, and the line isn't cited there either. (Also, Emperor? Charles hadn't been elected Holy Roman Emperor yet when Isabel was born...) Still, this sounds pretty plausible from what I've read- I'm fairly certain I read somewhere else that there were suspicions that Charles and his step-grandmother had an affair, at least, though I haven't seen the part about her referring to the daughter as "Infanta Isabel" elsewhere. So... it could definitely use a cite better than the lame "among historians," but I suspect it is in fact true. SnowFire (talk) 20:32, 21 April 2009 (UTC)[reply]
Geoffrey Parker in his recent biography Charles V (Emperor, A New Life of Charles V, 2019) essentially calls it nonsense. He traces the weak evidence used by some authors to make this sensational claim and shows why it is wrong. He categorically states the emperor did not sire a daughter with Germaine. Glendoremus (talk) 18:40, 6 March 2023 (UTC)[reply]