Talk:Affinity chromatography

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Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 16:55, 17 January 2022 (UTC)[reply]

Untitled

Removed Link Directory Spam Bioinformin

"pseudoaffinity chromatography" should this be discused here ?

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Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 13:29, 16 January 2022 (UTC)[reply]

Lectin Affinity?

I think a section on Lectin affinity chromatography would be good somewhere here. However, I don't know enough about it and I don't have time today. I have removed a brief mention of Lectin Affinity from the IMAC section. --Fatsug 00:58, 6 September 2007 (UTC)[reply]

Ligand exchange?

Affinity chromatography seems to be the same thing, except re-invented by biochemists instead. 129.97.246.125 (talk) 00:06, 10 February 2011 (UTC)[reply]

Problems with clarity

I have just shortened and clarified the lead section and added a reference to Bio-Rad's page on affinity chromatography (they are a major player in this field). With all due respect to the previous authors, this page is a train wreck and needs major revision to improve clarity and simplicity. I am going to make edits to this page over the coming weeks and will update this talk page as I do so. WeirdNAnnoyed (talk) 00:48, 15 September 2020 (UTC)[reply]

Dye-ligand affinity chromatography

I aim to create a new article about this what do you think?

Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography, but using dyes to apply on a support matrix of a column as the stationary phase that will allow a range of proteins with similar active sites to bind to, refers to as pseudo-affinity. ^[1] Synthetic dyes are used to mimic substrates binding to the active sites of proteins which can be further enhanced to target more specific proteins. Follow with washing, the process of removing other non-target molecules, then eluting out target proteins. The column can be reused many times due to the stability of immobilized dyes. It can carry out in a conventional way by using a column or in high-performance liquid chromatography (HPLC).^[2]

The dyes are immobilized on the column matrix effectively, since usually the dyes link to a monochlorotriazine or dichlorotriazine ring (triazine dye). This type of dyes works well especially on a support matrix with hydroxyl group such as cross-linked agarose.^[3] The dyes used in this type of chromatography are inexpensive and generally available as they are from textile industries called reactive dye. It contains chromophores that are often attached to a triazine ring.

Discovery

The serendipity discovery of dye-ligand ability is from a blue dye called blue dextran. The blue dye is used as a void volume marker for a gel filtration column. It shown that the dye has a property to bind to some certain proteins like pyruvate kinase and elute out with the void volume. Later, it was found that "cibacron blue FG-3A", reactive dye link to dextran, is respondsible for the interaction with the proteins.^[1]^[2]

References

^ ^a ^b McGettrick, Anne; Worrall, Margaret (2004). Dye-Ligand Affinity Chromatography. Totowa, NJ: Humana Press. p. 151-157. ISBN 978-1-59259-655-3. {{cite book}}: |access-date= requires |url= (help)
^ ^a ^b Hsu, James; Boyer, Philip (1993). Protein Purification by Dye-Ligand Chromatography (PDF). Springer. Retrieved 14 February 2021.
^ Denizli, Adil; Pişkin, Erhan (30 October 2001). "Dye-ligand affinity systems". Journal of Biochemical and Biophysical Methods. 49 (1–3): 391-416. doi:10.1016/s0165-022x(01)00209-3. {{cite journal}}: |access-date= requires |url= (help)

— Preceding unsigned comment added by U6181299 (talk • contribs) 06:53, 15 February 2021 (UTC)[reply]

Missing page in a citation

I found an incomplete citation for the affinity chromatography. I will add the page.Pinguin destept (talk) 19:21, 15 May 2023 (UTC)[reply]

[r1-1] McGettrick, Anne; Worrall, Margaret (2004). Dye-Ligand Affinity Chromatography. Totowa, NJ: Humana Press. p. 151-157. ISBN 978-1-59259-655-3. {{cite book}}: |access-date= requires |url= (help)

[r2-2] Hsu, James; Boyer, Philip (1993). Protein Purification by Dye-Ligand Chromatography (PDF). Springer. Retrieved 14 February 2021.

[3] Denizli, Adil; Pişkin, Erhan (30 October 2001). "Dye-ligand affinity systems". Journal of Biochemical and Biophysical Methods. 49 (1–3): 391-416. doi:10.1016/s0165-022x(01)00209-3. {{cite journal}}: |access-date= requires |url= (help)

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