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SpAB protein domain

From Wikipedia, the free encyclopedia
SpAB protein domain
Staphylococcal Protein A, b-domain
Identifiers
SymbolB
PfamPF02216
InterProIPR003132
SMARTTBC
PROSITEPDOC00957
MEROPSS12
SCOP21ehx / SCOPe / SUPFAM
TCDB9.B.4
CAZyGH58
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

In molecular biology, the domain B, refers to the immunoglobulin-binding domain found in the Staphylococcus aureus virulence factor protein A (SpA). Hence, it is abbreviated to SpAB.

Function

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SpAB enables theStaphylococcus aureus bacteria to evade the host's immune system through the disruption of opsonization and phagocytosis. It does this though SpAB binding to the Fc fragment of IgG.

Structure

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The B domain of SpA (SpAB) consists of three a-helices which are retained upon interaction with the Fc fragment of IgG. Protein A contains five highly homologous immunoglobulin (Ig)-binding domains in tandem (designated domains E, D, A, B and C), which share a common structure consisting of three helices in a closed left-handed twist. Protein A can exist in both secreted and membrane-bound forms, and has two distinct Ig-binding activities: each domain can bind Fc-gamma (the constant region of IgG involved in effector functions) and Fab (the Ig fragment responsible for antigen recognition).[1]

The native state of the B domain, deviates a lot since its inter-helical angles fluctuate. It appears to be relatively thermodynamically more stable than the E domain. The increased stability of the B domain may be due to heightened mobility, and therefore entropy, in the native state and decreased mobility entropy in the more compact denatured state.[2]

References

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  1. ^ Graille M, Stura EA, Corper AL, Sutton BJ, Taussig MJ, Charbonnier JB, Silverman GJ (May 2000). "Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: structural basis for recognition of B-cell receptors and superantigen activity". Proc. Natl. Acad. Sci. U.S.A. 97 (10): 5399–404. Bibcode:2000PNAS...97.5399G. doi:10.1073/pnas.97.10.5399. PMC 25840. PMID 10805799.
  2. ^ Alonso DO, Daggett V (2000). "Staphylococcal protein A: unfolding pathways, unfolded states, and differences between the B and E domains". Proc Natl Acad Sci U S A. 97 (1): 133–8. doi:10.1073/pnas.97.1.133. PMC 26628. PMID 10618383.
This article incorporates text from the public domain Pfam and InterPro: IPR003132