PFKM
6-phosphofructokinase, muscle type is an enzyme that in humans is encoded by the PFKM gene on chromosome 12. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009][4]
Structure
[edit]Gene
[edit]This gene is found on chromosome 12.[4] The coding region in PFKM only shares a 68% similarity with that of the liver-type PFKL.[5]
Protein
[edit]This 85-kDa protein is one of two subunit types that comprise the seven tetrameric PFK isozymes.[6][7] The muscle isozyme (PFK-1) is composed solely of PFKM.[6][8][9] The liver PFK (PFK-5) contains solely the second subunit type, PFKL, while the erythrocyte PFK includes five isozymes composed of different combinations of PFKM and PFKL.[6][7][9] These subunits evolved from a common prokaryotic ancestor via gene duplication and mutation events. Generally, the N-terminal of the subunits carries out their catalytic activity while the C-terminal contains allosteric ligand binding sites.[10] In particular, the binding site for the PFK inhibitor citrate is found in the PFKL C-terminal region.[11]
Function
[edit]This gene encodes one of three protein subunits of PFK, which are expressed and combined to form the tetrameric PFK in a tissue-specific manner. As a PFK subunit, PFKL is involved in catalyzing the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate. This irreversible reaction serves as the major rate-limiting step of glycolysis.[6][9][10][12]
Though the PFKM subunit majorly incorporates into muscle and erythrocyte PFKs, PFKM also is expressed in the heart, brain, and testis.[13]
Clinical significance
[edit]As the erythrocyte PFK is composed of both PFKL and PFKM, this heterogeneic composition is attributed with the differential PFK activity and organ involvement observed in some inherited PFK deficiency states in which myopathy or hemolysis or both can occur, such as glycogenosis type VII, also known as Tarui disease.[6][9][14] Notably, mutations in PFKM have been shown to cause Tarui disease due to homozygosity for catalytically inactive M subunits.[7][14] PFKM is confirmed to be involved in muscle PFK deficiency with early-onset hyperuricemia.[7]
Even though PFKM functions to drive glycolysis, its overexpression has been associated with type 2 diabetes and insulin resistance in skeletal muscle. One possible explanation suggests that the overexpression is meant to compensate for the allosteric inhibition of PFK1 as a result of excess oxidation of free fatty acids and accumulation of citrate and acetyl-CoA.[14]
Interactions
[edit]PFKM has been shown to interact with ATP6V0A4.[15]
Interactive pathway map
[edit]Click on genes, proteins and metabolites below to link to respective articles.[§ 1]
- ^ The interactive pathway map can be edited at WikiPathways: "GlycolysisGluconeogenesis_WP534".
See also
[edit]References
[edit]- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000033065 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: PFKM phosphofructokinase, muscle".
- ^ Levanon D, Danciger E, Dafni N, Bernstein Y, Elson A, Moens W, Brandeis M, Groner Y (Dec 1989). "The primary structure of human liver type phosphofructokinase and its comparison with other types of PFK". DNA. 8 (10): 733–43. doi:10.1089/dna.1989.8.733. PMID 2533063.
- ^ a b c d e Vora S, Seaman C, Durham S, Piomelli S (Jan 1980). "Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system". Proceedings of the National Academy of Sciences of the United States of America. 77 (1): 62–6. Bibcode:1980PNAS...77...62V. doi:10.1073/pnas.77.1.62. PMC 348208. PMID 6444721.
- ^ a b c d Vora S, Davidson M, Seaman C, Miranda AF, Noble NA, Tanaka KR, Frenkel EP, Dimauro S (Dec 1983). "Heterogeneity of the molecular lesions in inherited phosphofructokinase deficiency". The Journal of Clinical Investigation. 72 (6): 1995–2006. doi:10.1172/JCI111164. PMC 437040. PMID 6227635.
- ^ Koster JF, Slee RG, Van Berkel TJ (Apr 1980). "Isoenzymes of human phosphofructokinase". Clinica Chimica Acta; International Journal of Clinical Chemistry. 103 (2): 169–73. doi:10.1016/0009-8981(80)90210-7. PMID 6445244.
- ^ a b c d Musumeci O, Bruno C, Mongini T, Rodolico C, Aguennouz M, Barca E, Amati A, Cassandrini D, Serlenga L, Vita G, Toscano A (Apr 2012). "Clinical features and new molecular findings in muscle phosphofructokinase deficiency (GSD type VII)". Neuromuscular Disorders. 22 (4): 325–30. doi:10.1016/j.nmd.2011.10.022. PMID 22133655. S2CID 20133199.
- ^ a b Brüser A, Kirchberger J, Kloos M, Sträter N, Schöneberg T (May 2012). "Functional linkage of adenine nucleotide binding sites in mammalian muscle 6-phosphofructokinase". The Journal of Biological Chemistry. 287 (21): 17546–53. doi:10.1074/jbc.M112.347153. PMC 3366854. PMID 22474333.
- ^ Usenik A, Legiša M (23 November 2010). "Evolution of allosteric citrate binding sites on 6-phosphofructo-1-kinase". PLOS ONE. 5 (11): e15447. Bibcode:2010PLoSO...515447U. doi:10.1371/journal.pone.0015447. PMC 2990764. PMID 21124851.
- ^ Graham DB, Becker CE, Doan A, Goel G, Villablanca EJ, Knights D, Mok A, Ng AC, Doench JG, Root DE, Clish CB, Xavier RJ (21 July 2015). "Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst". Nature Communications. 6: 7838. Bibcode:2015NatCo...6.7838G. doi:10.1038/ncomms8838. PMC 4518307. PMID 26194095.
- ^ Kahn A, Meienhofer MC, Cottreau D, Lagrange JL, Dreyfus JC (Apr 1979). "Phosphofructokinase (PFK) isozymes in man. I. Studies of adult human tissues". Human Genetics. 48 (1): 93–108. doi:10.1007/bf00273280. PMID 156693. S2CID 23300861.
- ^ a b c Keildson S, Fadista J, Ladenvall C, Hedman ÅK, Elgzyri T, Small KS, Grundberg E, Nica AC, Glass D, Richards JB, Barrett A, Nisbet J, Zheng HF, Rönn T, Ström K, Eriksson KF, Prokopenko I, Spector TD, Dermitzakis ET, Deloukas P, McCarthy MI, Rung J, Groop L, Franks PW, Lindgren CM, Hansson O (Mar 2014). "Expression of phosphofructokinase in skeletal muscle is influenced by genetic variation and associated with insulin sensitivity". Diabetes. 63 (3): 1154–65. doi:10.2337/db13-1301. PMC 3931395. PMID 24306210.
- ^ Su Y, Zhou A, Al-Lamki RS, Karet FE (May 2003). "The a-subunit of the V-type H+-ATPase interacts with phosphofructokinase-1 in humans". The Journal of Biological Chemistry. 278 (22): 20013–8. doi:10.1074/jbc.M210077200. PMID 12649290.
Further reading
[edit]- Raben N, Sherman JB (1995). "Mutations in muscle phosphofructokinase gene". Human Mutation. 6 (1): 1–6. doi:10.1002/humu.1380060102. PMID 7550225. S2CID 46649815.
- Kahn A, Etiemble J, Meienhofer MC, Bovin P (Jun 1975). "Erythrocyte phosphofructokinase deficiency associated with an unstable variant of muscle phosphofructokinase". Clinica Chimica Acta; International Journal of Clinical Chemistry. 61 (3): 415–9. doi:10.1016/0009-8981(75)90434-9. PMID 125160.
- Zhao ZZ, Malencik DA, Anderson SR (Feb 1991). "Protein-induced inactivation and phosphorylation of rabbit muscle phosphofructokinase". Biochemistry. 30 (8): 2204–16. doi:10.1021/bi00222a026. PMID 1825608.
- Yamasaki T, Nakajima H, Kono N, Hotta K, Yamada K, Imai E, Kuwajima M, Noguchi T, Tanaka T, Tarui S (Aug 1991). "Structure of the entire human muscle phosphofructokinase-encoding gene: a two-promoter system". Gene. 104 (2): 277–82. doi:10.1016/0378-1119(91)90262-A. PMID 1833270.
- Sharma PM, Reddy GR, Babior BM, McLachlan A (Jun 1990). "Alternative splicing of the transcript encoding the human muscle isoenzyme of phosphofructokinase". The Journal of Biological Chemistry. 265 (16): 9006–10. doi:10.1016/S0021-9258(19)38803-9. PMID 2140567.
- Nakajima H, Kono N, Yamasaki T, Hotta K, Kawachi M, Kuwajima M, Noguchi T, Tanaka T, Tarui S (Jun 1990). "Genetic defect in muscle phosphofructokinase deficiency. Abnormal splicing of the muscle phosphofructokinase gene due to a point mutation at the 5'-splice site". The Journal of Biological Chemistry. 265 (16): 9392–5. doi:10.1016/S0021-9258(19)38861-1. PMID 2140573.
- Valdez BC, Chen Z, Sosa MG, Younathan ES, Chang SH (Mar 1989). "Human 6-phosphofructo-1-kinase gene has an additional intron upstream of start codon". Gene. 76 (1): 167–9. doi:10.1016/0378-1119(89)90019-X. PMID 2526044.
- Sharma PM, Reddy GR, Vora S, Babior BM, McLachlan A (Apr 1989). "Cloning and expression of a human muscle phosphofructokinase cDNA". Gene. 77 (1): 177–83. doi:10.1016/0378-1119(89)90372-7. PMID 2526045.
- Nakajima H, Noguchi T, Yamasaki T, Kono N, Tanaka T, Tarui S (Oct 1987). "Cloning of human muscle phosphofructokinase cDNA". FEBS Letters. 223 (1): 113–6. doi:10.1016/0014-5793(87)80519-7. PMID 2822475. S2CID 42849336.
- Vora S, Seaman C, Durham S, Piomelli S (Jan 1980). "Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system". Proceedings of the National Academy of Sciences of the United States of America. 77 (1): 62–6. Bibcode:1980PNAS...77...62V. doi:10.1073/pnas.77.1.62. PMC 348208. PMID 6444721.
- Kahn A, Weil D, Cottreau D, Dreyfus JC (Feb 1981). "Muscle phosphofructokinase deficiency in man: expression of the defect in blood cells and cultured fibroblasts". Annals of Human Genetics. 45 (Pt 1): 5–14. doi:10.1111/j.1469-1809.1981.tb00300.x. PMID 6459054. S2CID 39444994.
- Vasconcelos O, Sivakumar K, Dalakas MC, Quezado M, Nagle J, Leon-Monzon M, Dubnick M, Gajdusek DC, Goldfarb LG (Oct 1995). "Nonsense mutation in the phosphofructokinase muscle subunit gene associated with retention of intron 10 in one of the isolated transcripts in Ashkenazi Jewish patients with Tarui disease". Proceedings of the National Academy of Sciences of the United States of America. 92 (22): 10322–6. Bibcode:1995PNAS...9210322V. doi:10.1073/pnas.92.22.10322. PMC 40788. PMID 7479776.
- Tsujino S, Servidei S, Tonin P, Shanske S, Azan G, DiMauro S (May 1994). "Identification of three novel mutations in non-Ashkenazi Italian patients with muscle phosphofructokinase deficiency". American Journal of Human Genetics. 54 (5): 812–9. PMC 1918246. PMID 7513946.
- Raben N, Exelbert R, Spiegel R, Sherman JB, Nakajima H, Plotz P, Heinisch J (Jan 1995). "Functional expression of human mutant phosphofructokinase in yeast: genetic defects in French Canadian and Swiss patients with phosphofructokinase deficiency". American Journal of Human Genetics. 56 (1): 131–41. PMC 1801305. PMID 7825568.
- Raben N, Sherman J, Miller F, Mena H, Plotz P (Mar 1993). "A 5' splice junction mutation leading to exon deletion in an Ashkenazic Jewish family with phosphofructokinase deficiency (Tarui disease)". The Journal of Biological Chemistry. 268 (7): 4963–7. doi:10.1016/S0021-9258(18)53489-X. PMID 8444874.
- Howard TD, Akots G, Bowden DW (May 1996). "Physical and genetic mapping of the muscle phosphofructokinase gene (PFKM): reassignment to human chromosome 12q". Genomics. 34 (1): 122–7. doi:10.1006/geno.1996.0250. PMID 8661033.
- Hamaguchi T, Nakajima H, Noguchi T, Nakagawa C, Kuwajima M, Kono N, Tarui S, Matsuzawa Y (1997). "Novel missense mutation (W686C) of the phosphofructokinase-M gene in a Japanese patient with a mild form of glycogenosis VII". Human Mutation. 8 (3): 273–5. doi:10.1002/(SICI)1098-1004(1996)8:3<273::AID-HUMU13>3.0.CO;2-#. PMID 8889589. S2CID 196597214.
- Scherer PE, Lisanti MP (Aug 1997). "Association of phosphofructokinase-M with caveolin-3 in differentiated skeletal myotubes. Dynamic regulation by extracellular glucose and intracellular metabolites". The Journal of Biological Chemistry. 272 (33): 20698–705. doi:10.1074/jbc.272.33.20698. PMID 9252390.
- Ristow M, Vorgerd M, Möhlig M, Schatz H, Pfeiffer A (Dec 1997). "Deficiency of phosphofructo-1-kinase/muscle subtype in humans impairs insulin secretion and causes insulin resistance". The Journal of Clinical Investigation. 100 (11): 2833–41. doi:10.1172/JCI119831. PMC 508489. PMID 9389749.