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Norbert Perrimon

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Norbert Perrimon
Born (1958-10-24) October 24, 1958 (age 66) [2]
Bosguérard-de-Marcouville[2]
NationalityFrench
CitizenshipFrance, United States
Alma materUniversity of Paris
Known forGAL4/UAS system
AwardsGeorge W. Beadle Award (2004)[1]
Scientific career
Institutions
ThesisAnalyse Clonale de Mutations en Lignee Germinale chez la Drosophile (1983)
Academic advisorsMadeleine Gans
Notable studentsSara Cherry
Website

Norbert Perrimon is a French geneticist and developmental biologist. He is the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School, an Investigator at the Howard Hughes Medical Institute, and an Associate of the Broad Institute. He is known for developing a number of techniques for used in genetic research with Drosophila melanogaster, as well as specific substantive contributions to signal transduction, developmental biology and physiology.

Education

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Perrimon was born in 1958 in Bosguérard-de-Marcouville, France. He earned his undergraduate degree (Maitrise of Biochemistry) at the University of Paris VI, in 1981, then completed his doctorate in 1983 with Madeleine Gans, also at the University of Paris.

Career

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From 1983 to 1986 Perrimon was a postdoctoral researcher with Anthony Mahowald[3][4][5][6] at Case Western Reserve University, and in 1986 at the age of 27 he accepted an appointment as faculty at Harvard Medical School. He is currently the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School. He has been an Investigator of the Howard Hughes Medical Institute since 1986.[7]

Research

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Perrimon's group developed many methods that have significantly improved the Drosophila toolbox. Perrimon co-developed the GAL4/UAS system method with Andrea Brand to control gene expression in Drosophila.[8] This method has been described as “a fly geneticist's Swiss army knife”[9] and is widely used in Drosophila genetics. Together with Tze-bin Chou, he developed the FLP-FRT DFS method to generate germline mosaics, a method that allowed the large-scale characterization of the maternal effect of zygotic lethal mutations.[10][11][12] He developed and improved methods in vivo RNAi with Janquan Ni.[13][14][15] His lab has pioneered high-throughput whole-genome RNAi screening to interrogate systematically the function of all fly genes in various cell-based assays.[16][17][18][19][20][21][22] With Ram Viswanatha, he developed CRISPR/Cas9 pooled screens in Drosophila cells to facilitate large-scale screen in Drosophila and other arthropod cell lines.[23] The approach is particularly powerful to identify the mechanism of entry of toxins.[24]

In 2003 he created the Drosophila RNAi Screening Center at Harvard Medical School and in 2008, he initiated the Transgenic RNAi Project to generate transgenic RNAi lines for the community using optimized shRNA vectors that his lab developed.

Awards and honors

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Perrimon was elected to the United States National Academy of Sciences in April 2013,[18][25] after naturalizing as an American citizen.

References

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  1. ^ a b Schüpbach, T. (2004). "The 2004 George W. Beadle Medal". Genetics. 166 (2): 649–650. doi:10.1534/genetics.166.2.649. PMC 1470725. PMID 15020455.
  2. ^ a b "Archived copy". Archived from the original on 2014-08-08. Retrieved 2014-08-07.{{cite web}}: CS1 maint: archived copy as title (link)
  3. ^ Perrimon, N; Engstrom, L; Mahowald, A. P. (1985). "Developmental genetics of the 2C-D region of the Drosophila X chromosome". Genetics. 111 (1): 23–41. doi:10.1093/genetics/111.1.23. PMC 1202596. PMID 3928431.
  4. ^ Perrimon, N; Mohler, D; Engstrom, L; Mahowald, A. P. (1986). "X-linked female-sterile loci in Drosophila melanogaster". Genetics. 113 (3): 695–712. doi:10.1093/genetics/113.3.695. PMC 1202863. PMID 3089870.
  5. ^ Perrimon, N; Mahowald, A. P. (1986). "L(1)hopscotch, A larval-pupal zygotic lethal with a specific maternal effect on segmentation in Drosophila". Developmental Biology. 118 (1): 28–41. doi:10.1016/0012-1606(86)90070-9. PMID 3095163.
  6. ^ Perrimon, N; Mahowald, A. P. (1987). "Multiple functions of segment polarity genes in Drosophila". Developmental Biology. 119 (2): 587–600. doi:10.1016/0012-1606(87)90061-3. PMID 3803719.
  7. ^ "Norbert Perrimon, PhD | HHMI.org". HHMI.org. Retrieved 2016-11-21.
  8. ^ Brand, A. H.; Perrimon, N. (1993). "Targeted gene expression as a means of altering cell fates and generating dominant phenotypes". Development. 118 (2): 401–415. doi:10.1242/dev.118.2.401. PMID 8223268.
  9. ^ Shetty, P. (2008). "Molecular biologist Andrea Brand: encouraging women in science". The Lancet. 371 (9617): 979. doi:10.1016/S0140-6736(08)60439-0. PMID 18358916. S2CID 33668220.
  10. ^ Chou, T. B.; Perrimon, N. (1992-07-01). "Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila". Genetics. 131 (3): 643–653. doi:10.1093/genetics/131.3.643. ISSN 0016-6731. PMC 1205036. PMID 1628809.
  11. ^ Chou, T. B.; Noll, E.; Perrimon, N. (1993-12-01). "Autosomal P[ovoD1] dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras". Development. 119 (4): 1359–1369. doi:10.1242/dev.119.4.1359. ISSN 0950-1991. PMID 8306893.
  12. ^ Chou, Tze-bin; Perrimon, Norbert (1996-12-01). "The Autosomal FLP-DFS Technique for Generating Germline Mosaics in Drosophila melanogaster". Genetics. 144 (4): 1673–1679. doi:10.1093/genetics/144.4.1673. ISSN 0016-6731. PMC 1207718. PMID 8978054.
  13. ^ Ni, Jian-Quan; Markstein, Michele; Binari, Richard; Pfeiffer, Barret; Liu, Lu-Ping; Villalta, Christians; Booker, Matthew; Perkins, Lizabeth; Perrimon, Norbert (2008-01-01). "Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster". Nature Methods. 5 (1): 49–51. doi:10.1038/nmeth1146. ISSN 1548-7091. PMC 2290002. PMID 18084299.
  14. ^ Ni, Jian-Quan; Liu, Lu-Ping; Binari, Richard; Hardy, Robert; Shim, Hye-Seok; Cavallaro, Amanda; Booker, Matthew; Pfeiffer, Barret D.; Markstein, Michele (2009-08-01). "A Drosophila resource of transgenic RNAi lines for neurogenetics". Genetics. 182 (4): 1089–1100. doi:10.1534/genetics.109.103630. ISSN 1943-2631. PMC 2728850. PMID 19487563.
  15. ^ Ni, Jian-Quan; Zhou, Rui; Czech, Benjamin; Liu, Lu-Ping; Holderbaum, Laura; Yang-Zhou, Donghui; Shim, Hye-Seok; Tao, Rong; Handler, Dominik (2011-05-01). "A genome-scale shRNA resource for transgenic RNAi in Drosophila". Nature Methods. 8 (5): 405–407. doi:10.1038/nmeth.1592. ISSN 1548-7105. PMC 3489273. PMID 21460824.
  16. ^ Boutros, Michael; Kiger, Amy A.; Armknecht, Susan; Kerr, Kim; Hild, Marc; Koch, Britta; Haas, Stefan A.; Paro, Renato; Perrimon, Norbert; Heidelberg Fly Array Consortium (2004-02-06). "Genome-wide RNAi analysis of growth and viability in Drosophila cells". Science. 303 (5659): 832–835. doi:10.1126/science.1091266. ISSN 1095-9203. PMID 14764878.
  17. ^ Perrimon, N. (2005). "Norbert Perrimon". Current Biology. 15 (13): R481–R482. doi:10.1016/j.cub.2005.06.050. PMID 16059997. S2CID 34889938.
  18. ^ a b Ravindran, S (2014). "Profile of Norbert Perrimon". Proceedings of the National Academy of Sciences. 111 (21): 7501–2. Bibcode:2014PNAS..111.7501R. doi:10.1073/pnas.1406464111. PMC 4040556. PMID 24778217.
  19. ^ Perrimon, N; Gans, M (1983). "Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237". Developmental Biology. 100 (2): 365–73. doi:10.1016/0012-1606(83)90231-2. PMID 6418585.
  20. ^ Perrimon, N (1984). "Clonal Analysis of Dominant Female-Sterile, Germline-Dependent Mutations in DROSOPHILA MELANOGASTER". Genetics. 108 (4): 927–39. doi:10.1093/genetics/108.4.927. PMC 1224274. PMID 17246244.
  21. ^ White, R. A.; Perrimon, N; Gehring, W. J. (1984). "Differentiation markers in the Drosophila ovary". Journal of Embryology and Experimental Morphology. 84: 275–86. PMID 6442733.
  22. ^ Perrimon, N.; Engstrom, L.; Mahowald, A. P. (1984). "The effects of zygotic lethal mutations on female germ-line functions in Drosophila". Developmental Biology. 105 (2): 404–414. doi:10.1016/0012-1606(84)90297-5. PMID 6479445.
  23. ^ Viswanatha, Raghuvir; Li, Zhongchi; Hu, Yanhui; Perrimon, Norbert (2018-07-27). "Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells". eLife. 7: e36333. doi:10.7554/eLife.36333. ISSN 2050-084X. PMC 6063728. PMID 30051818.
  24. ^ Xu, Ying; Viswanatha, Raghuvir; Sitsel, Oleg; Roderer, Daniel; Zhao, Haifang; Ashwood, Christopher; Voelcker, Cecilia; Tian, Songhai; Raunser, Stefan; Perrimon, Norbert; Dong, Min (October 2022). "CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor". Nature. 610 (7931): 349–355. doi:10.1038/s41586-022-05250-7. ISSN 1476-4687. PMC 9631961. PMID 36171290.
  25. ^ "April 30, 2013, NAS Election", National Academy of Sciences (last visited May 3, 2013).
  26. ^ Teltsch, Kathleen (1985-02-10). "16 ARE GIVEN MARKEY RESEARCH SCHOLARSHIPS". The New York Times. ISSN 0362-4331. Retrieved 2023-08-28.
  27. ^ "Innovator Award Recipients". www.expressgenes.com. GeneExpression Systems. Retrieved 31 October 2016.
  28. ^ "Member Directory | American Academy of Arts and Sciences". www.amacad.org. Retrieved 2023-09-12.
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