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Methidiumpropyl-EDTA

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Methidiumpropyl-EDTA
Names
IUPAC name
3,8-Diamino-6-[4-[13-carboxy-9,12-bis(carboxymethyl)-1,7-dioxo-2,6,9,12-tetraazatridec-1-yl]phenyl]-5-methylphenanthridinium
Other names
MPE
Identifiers
3D model (JSmol)
ECHA InfoCard 100.149.943 Edit this at Wikidata
  • O=C(O)CN(CC(=O)O)CCN(CC(=O)O)CC(=O)NCCCNC(=O)C1=CC=C(C=C1)C=2C3=CC(N)=CC=C3C=4C=CC(N)=CC4[N+]2C
Properties
C34H40N7O8
Molar mass 674.735 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Methidiumpropyl-EDTA (MPE) is composed of the DNA intercalator methidium covalently attached to the metal chelator ethylenediaminetetraacetic acid (EDTA) by a short linker.[1] MPE nonspecifically binds and cleaves DNA at multiple locations.

See also

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References

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  1. ^ Tanner, N.Kyle; Cech, Thomas R. (1985). "Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena:inhibition by methidiumpropyl-EDTA and localization of the major dye binding sites". Nucleic Acids Research. 13 (21): 7759–7779. doi:10.1093/nar/13.21.7759. PMC 322085. PMID 2415924.