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Leptotrichia goodfellowii

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Leptotrichia goodfellowii
Scientific classification
Domain:
Bacteria
Phylum:
Fusobacteriota
Class:
Fusobacteriia
Order:
Fusobacterales
Family:
Leptotrichiaceae
Genus:
Leptotrichia
Species:
L. goodfellowii
Binomial name
Leptotrichia goodfellowii
Eribe et al. 2004[1]
Type strain
CCUG 32286, CIP 107915, DSM 19756, JCM 16774, strain LB 57[2]

Leptotrichia goodfellowii (also known as Pseudoleptotrichia goodfellowii) is a Gram-negative, non-spore-forming and non-motile anaerobic bacterium from the genus of Leptotrichia which has been isolated from human blood of an endocarditis patient.[1][2][3][4][5] It is associated with infections in the oral cavity and respiratory tract. While primarily associated with opportunistic infections in immunocompromised individuals, its exact pathogenic mechanisms and clinical significance warrant further investigation.

Basic information

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L. goodfellowii is an anaerobic, non-spore forming, gram-negative bacillus. It is typically found in blood, and when it was first found, it was plated on blood agar and incubated at 37 °C.[6] It was gram-stained and analyzed via microscopy. It is rare, but when seen, it is a human pathogen typically in immunocompromised patients.[7] It can cause endocarditis, which is inflammation of the heart chambers and valves, making it important to study, so we can learn how to best treat this condition. Leptotrichia spp are common in many human infections like bite wounds and bloodstream infections.[8] Other phylogenies include Leptotrichia buccalis, hofstadii, hongkongensis, massiliensis, shahii, trevisanii, and wadei. Each of these neighbors can cause similar human infections, as they are all closely related.[9]

Entymology

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In 1683, Antonie Van Leeuwehhoek first discovered Leptotrichia, and the species L. goodfellowii was named in honor of Mike Goodfellow's significant contributions to microbial systematics.[10]

Reclassification

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After 16S rRNA gene sequence analysis, it was found that the six other strains of Leptotrichia were significantly more closely related than L. goodfellowii.[11] This led Eisenberg et al. to propose reclassifying L. goodfellowii into its own genus, Pseudoleptotrichia. This proposal was validly published in 2020.

Taxonomy and phylogeny

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Below are its taxonomic classifications and a partial phylogenetic tree of L. goodfellowii.[11][12] The Leptotrichiaceae family consists of four genera, Streptobacillus, Sneathia, Sebaldella, and Leptotrichia.[13] This entire family is fairly understudied, but research continues to be done about its genera. Streptobacillus have been implicated in causing disease, notably a type of rat-bite fever known as haverhill fever.[14] Sneathia species have been found to have adverse pathogenic effects on the female reproductive system.[15] Sebaldella has been isolated from insect intestinal tracts and its metabolic activity shows evidence of supplying organic nitrogen to its insect host.[13]

Taxonomy Box
Domain Bacteria
Phylum Fusobacteriota
Class Fusobacteriia
Order Fusobacteriales
Family Leptotrichiaceae
Genus Pseudoleptotrichia

Genomics

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L. goodfellowii is hard to sequence by conventional methods, so molecular detection using 16S rDNA PCA followed by Sanger sequencing is most effective.[8] The genome has been sequenced and is a mean total length of 2.28 Mb, with 2,199 genes, and 2,079 proteins.[10] When conducting genomic analyses, the study conducted by Liebermann et al. worked with a species-level identification threshold of ~99.6%–99.7% similarity, and no significantly similar species were found; the next closest related species was canine oral Leptotrichia spp, which had similarity of 87.81%-89.57%.[8]

Ecology

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In regards to ecology, Leptotrichia can be found in bacterial biofilms, especially in the human oral cavity.[16] L. goodfellowii has been found to colonize the human oropharynx and has also been isolated in human blood.[8] Furthermore, L. goodfellowii has been isolated from a variety of sources, including oral swabs of guinea pigs, gastric fluid of patients who had stillborn child expulsion, and from the secretions of a healthy patient's dog bite wound.[6]

Metabolism

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L. goodfellowii are anaerobic chemoheterotrophic bacteria and are mesophilic, as they grow optimally at 37 °C. All Leptotrichia species ferment carbohydrates like glucose, maltitol, maltose, and lactose, and produce lactic acid as part of their metabolic pathways.[6] A unique property of L. goodfellowii is that it can utilize sucrose isomers that other oral-bacteria like cannot.[16] The DNA sequence of another Leptotrichia species, L. buccalis, contained the phosphoenolpyruvate:carbohydrate phosphotransferase (PEP-PTS) operon, which can be extrapolated to assume that L. goodfellowii would also include the PEP-PTS operon, as their metabolic activities are similar.[16]

Pathology

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L. goodfellowii must be detected molecularly, but is not always accurate due to poor or contaminated DNA quality. In one case study, a 66-year-old woman had culture-negative endocarditis. For her, 16S Sanger sequencing was not effective due to the above reasons.[8] Researchers deconvoluted the sample using amplicon sequencing, which then allowed for appropriate pathologic diagnosis that her endocarditis was caused by L. goodfellowii.[8] The best treatment for endocarditis caused by L. goodfellowii is β-lactam antibiotics. Most times, this disease has a fast onset and short but extreme course. In this case study, the patient did die, however.[8]

References

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[15] [14]

[13] [11] [10] [6] [8] [7] [12] [16] [9]

  1. ^ a b Parte, A.C. "Leptotrichia". LPSN.
  2. ^ a b "Leptotrichia goodfellowii". www.uniprot.org.
  3. ^ "Details: DSM-19756". www.dsmz.de.
  4. ^ Lim, YK; Kweon, OJ; Kim, HR; Lee, MK (Winter 2016). "Leptotrichia goodfellowii Infection: Case Report and Literature Review". Annals of Clinical and Laboratory Science. 46 (1): 83–6. PMID 26927348.
  5. ^ Liu, Dongyou (2011). Molecular Detection of Human Bacterial Pathogens. CRC Press. ISBN 9781439812389.
  6. ^ a b c d Eribe, E. R. K., Paster, B. J., Caugant, D. A., Dewhirst, F. E., Stromberg, V. K., Lacy, G. H., & Olsen, I. (2004). Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. Nov., Leptotrichia hofstadii sp. Nov., Leptotrichia shahii sp. Nov. And Leptotrichia wadei sp. Nov. International Journal of Systematic and Evolutionary Microbiology, 54(2), 583–592. https://doi.org/10.1099/ijs.0.02819-0
  7. ^ a b Lim YK, Kweon OJ, Kim HR, Lee MK. Leptotrichia goodfellowii Infection: Case Report and Literature Review. Ann Clin Lab Sci. 2016 Winter;46(1):83-6. PMID 26927348.
  8. ^ a b c d e f g h Lieberman, J. A., Kurosawa, K., SenGupta, D., Cookson, B. T., Salipante, S. J., & Busch, D. (2021). Identification of Leptotrichia goodfellowii infective endocarditis by next-generation sequencing of 16S rDNA amplicons. Cold Spring Harbor Molecular Case Studies, 7(1), a005876. https://doi.org/10.1101/mcs.a005876
  9. ^ a b Wilfredo R. Matias, Daniel L. Bourque, Tomoko Niwano, Andrew B. Onderdonk, Joel T. Katz, "Subacute Bacterial Endocarditis with Leptotrichia goodfellowii in a Patient with a Valvular Allograft: A Case Report and Review of the Literature", Case Reports in Infectious Diseases, vol. 2016, Article ID 3051212, 5 pages, 2016. https://doi.org/10.1155/2016/3051212
  10. ^ a b c Emenike R. K. Eribe & Ingar Olsen (2017) Leptotrichia species in human infections II, Journal of Oral Microbiology,9:1, DOI: 10.1080/20002297.2017.1368848
  11. ^ a b c Eisenberg, T., Glaeser, S. P., Blom, J., & Kämpfer, P. (2020). Proposal to reclassify Leptotrichia goodfellowii into a novel genus as Pseudoleptotrichia goodfellowii gen. Nov., comb. Nov. International Journal of Systematic and Evolutionary Microbiology, 70(3), 2084–2088. https://doi.org/10.1099/ijsem.0.004024
  12. ^ a b Schoch, C. L., Ciufo, S., Domrachev, M., Hotton, C. L., Kannan, S., Khovanskaya, R., Leipe, D., Mcveigh, R., O'Neill, K., Robbertse, B., Sharma, S., Soussov, V., Sullivan, J. P., Sun, L., Turner, S., & Karsch-Mizrachi, I. (2020). NCBI Taxonomy: A comprehensive update on curation, resources and tools. Database, 2020, baaa062. https://doi.org/10.1093/database/baaa062
  13. ^ a b c Lory, S. (2014). The Family Leptotrichiaceae. In E. Rosenberg, E. F. DeLong, S. Lory, E. Stackebrandt, & F. Thompson (Eds.), The Prokaryotes: Firmicutes and Tenericutes (pp. 213–214). Springer. https://doi.org/10.1007/978-3-642-30120-9_357
  14. ^ a b Shanson, D. C., Gazzard, B. G., Midgley, J., Dixey, J., Gibson, G. L., Stevenson, J., Finch, R. G., & Cheesbrough, J. (1983). Streptobacillus moniliformis isolated from blood in four cases of Haverhill fever. Lancet, 2(8341), 92–94. https://doi.org/10.1016/s0140-6736(83)90072-7
  15. ^ a b Theis, K. R., Florova, V., Romero, R., Borisov, A. B., Winters, A. D., Galaz, J., & Gomez-Lopez, N. (2021). Sneathia: An emerging pathogen in female reproductive disease and adverse perinatal outcomes. Critical Reviews in Microbiology, 47(4), 517–542. https://doi.org/10.1080/1040841X.2021.1905606
  16. ^ a b c d Thompson, J., & Pikis, A. (2012). Metabolism of sugars by genetically diverse species of oral Leptotrichia. Molecular Oral Microbiology, 27(1), 34–44. https://doi.org/10.1111/j.2041-1014.2011.00627.x


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