Frank O. Bastian
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Frank O. Bastian is an American physician and neuropathologist who worked at Louisiana State University before moving to a university in New Orleans in 2019.[1][2] He specializes in transmissible spongiform encephalopathies (TSEs), which include bovine spongiform encephalopathy (BSE) in cattle, scrapie in sheep and goats, and Creutzfeldt–Jakob disease (CJD) in humans.[3]
Bastian challenges the widely popular theory that TSEs are caused by prions, which are composed of a misfolded form of major prion protein (PrP). He argues that TSEs are caused by an extremely small, cell wall deficient bacterium called Spiroplasma, and that prions are the result of the disease process. The lack of a cell wall means the bacterium is not susceptible to most common antibiotics such as penicillin, which target cell wall synthesis. Bastian first found Spiroplasma in the brain of a CJD patient in an autopsy in 1979.[4] He later demonstrated that Spiroplasma mirum strain SMCA (suckling mouse cataract agent) induces a microcystic spongiform encephalopathy in suckling rats that is similar to CJD. In 2007 he isolated Spiroplasma from scrapie and chronic wasting disease (a TSE affecting deer, elk, and moose, abbreviated CWD), cultured them, and inoculated them into sheep and deer.[citation needed]
According to Bastian, the scrapie and CWD Spiroplasma isolates caused a spongiform encephalopathy identical to scrapie and CWD.[5] Under his hypothesis, prion protein scrapie (PrPSc) is not the cause; it is merely an imperfect marker of infection (with both sensitivity and NPV <1), and is either induced by Spiroplasma directly or by a defence mechanism of the host.
In 2014 Bastian discovered that Spiroplasma forms biofilms which give them significant resistance to radiation, heat, and disinfectants. He, along with Laura Manuelidis of Yale University, who claims that TSEs are caused by an as yet undiscovered virus, are two of the main skeptics of the prion theory. Other scientists have so far been unable to duplicate his results, casting doubt on this hypothesis.[6][7] Bastian however maintains that the inability to detect Spiroplasma in 100% of cases stems from genetic variability.[8]
Publications
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See also
[edit]References
[edit]- ^ "Can CWD Really be Cured?". 28 February 2019.
- ^ "Frank Bastian". LSU AgCenter. 2013-01-02. Retrieved 2016-01-09.
- ^ “MultiBriefs:” Professor claims cure for CWD, but others aren't sure”
- ^ Bastian, F. O. (2015-09-28). "Spiroplasma-like inclusions in Creutzfeldt-Jakob disease". Arch Pathol Lab Med. 103 (13): 665–9. PMID 389196.
- ^ Bastian, FO; Sanders, DE; Forbes, WA; et al. (2015-09-28). "Spiroplasma spp. from transmissible spongiform encephalopathy brains or ticks induce spongiform encephalopathy in ruminants". J. Med. Microbiol. 56 (9): 1235–42. doi:10.1099/jmm.0.47159-0. PMID 17761489.
- ^ Leach, RH; Matthews, WB; Will, R (2015-09-28). "Creutzfeldt-Jakob disease. Failure to detect spiroplasmas by cultivation and serological tests". J. Neurol. Sci. 59 (3): 349–53. doi:10.1016/0022-510x(83)90020-5. PMID 6348215. S2CID 3558955.
- ^ "Absence of Spiroplasma or Other Bacterial 16S rRNA Genes in Brain Tissue of Hamsters with Scrapie". Jcm.asm.org. Retrieved 2016-01-09.
- ^ Bastian, Frank O.; Foster, John W. (2001). "Spiroplasma sp.16S rDNA in Creutzfeldt-Jakob Disease and Scrapie as Shown by PCR and DNA Sequence Analysis". Journal of Neuropathology & Experimental Neurology. 60 (6): 613–620. doi:10.1093/jnen/60.6.613. PMID 11398837 – via ResearchGate.