Draft:MIDORI Green DNA dyes
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Comment: Please remove the external links from the body of the article in the headers. Also, this reads promotionally and needs citations for the "Fluorescence and detection" section. Right now, it feels like an advertisement for the NIPPON Genetics company. UserMemer (chat) Tribs 11:58, 26 October 2024 (UTC)
MIDORI Green is a family of fluorescent nucleic acid stains designed for the visualization of DNA in agarose gels during gel electrophoresis. These dyes offer an alternative to ethidium bromide (EtBr), a commonly used DNA stain that is mutagenic and hazardous. MIDORI Green dyes are safer alternatives, specifically formulated to reduce toxicity while maintaining or improving sensitivity in DNA detection.
Overview
[edit]MIDORI Green DNA dyes are non-carcinogenic and non-toxic alternatives[1][2][3][4] to EtBr for staining DNA. They are widely used in molecular biology labs for routine DNA electrophoresis due to their lower health risks.
Key Products
[edit]The MIDORI Green product line includes the following variants:
Description: Added directly to the agarose gel solution before polymerization, allowing DNA staining during the electrophoresis process without the need for post-staining.
Applications[5][6]: Time-saving, ideal for labs looking to avoid additional staining steps.
Description: Used for post-staining agarose gels after electrophoresis, offering high sensitivity for DNA detection.
Applications[7][8][9]: Useful when precise staining is required after running the gel.
Description: A high-sensitivity dye specifically designed to detect very low concentrations of DNA. It is suitable for applications where even faint DNA bands need to be visible.
Applications[10][11][12]: Ideal for detecting small quantities of DNA, such as in sensitive PCR experiments or low-yield DNA extractions.
Description: An easy-to-use variant that is directly compatible with both blue and UV light transilluminators, simplifying the process of gel imaging and visualization without compromising sensitivity.
Applications: Suitable for labs with both blue light and UV-based imaging systems, offering flexibility in the choice of detection equipment.
Applications
[edit]MIDORI Green dyes are widely used in various molecular biology applications, including:
- Agarose gel electrophoresis: The primary application for visualizing DNA fragments during routine molecular biology experiments.
- DNA fragment analysis: Used in the analysis of DNA fragment sizes for applications such as cloning, PCR diagnostics, and sequencing.
- Education: Frequently adopted in educational settings due to their safer profiles, reducing the risk of exposure to hazardous chemicals.
Safety and Environmental Impact
[edit]Compared to ethidium bromide, which is mutagenic and requires special handling and disposal procedures, MIDORI Green DNA dyes are designed to be non-carcinogenic and non-toxic[1][2][4]>. They are classified as non-hazardous waste, simplifying disposal and reducing the environmental footprint of laboratories.
Fluorescence and Detection
[edit]MIDORI Green dyes are designed to bind specifically to the phosphate backbone of nucleic acids, allowing for accurate visualization of DNA fragments. They fluoresce under UV, blue, and blue/green LED light sources, with optimal excitation at approximately 490 nm and emission around 520 nm, providing bright green fluorescence during or after electrophoresis. The dyes emit bright green fluorescence, easily visible to the naked eye or through imaging systems
The ability to visualize DNA under different types of light makes them highly versatile for use in various gel documentation systems.
References
[edit]- ^ a b NIPPON Genetics EUROPE. "MIDORIGreen Xtra DNA Stain Safety Report" (PDF).
- ^ a b NIPPON Genetics EUROPE. "MIDORI Green Direct DNA Stain Safety Report" (PDF).
- ^ NIPPON Genetics EUROPE. "MIDORI Green Advance DNA Stain Safety Report" (PDF).
- ^ a b NIPPON Genetics EUROPE. "MIDORIGreen Easy DNA Stain Safety Report" (PDF).
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