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Wikipedia:United States Education Program/Courses/JHU MolBio Ogg 2013/Group 81B

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Group 81B

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This is the Wikipedia page for 410.602 Molecular Biology, Spring, 2013, group 81B. This group will be working on the article Immunolabeling.

Use the talk page here to collaborate as a group, when learning to use and navigate Wikipedia, assessing articles, or for any other topic.

Use this page (not the talk page) for article assessments; rationale for selecting an article; etc.

Please create a new section here for each of those assignments.

Initial Article Assessments from Jbmontgomery24

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Histone octamer [1] [2] [3]

~ Article listed as a stub-class and high-importance via Molecular & Cellular Biology Portal
~ Needs a high degree of expansion on lead section of topic
~ No images to support topic
~ No supportive material for lead section
~ No references or inline citations
~ No discussions currently listed on the talk page of histone octamer

Silent Mutation [4] [5] [6]

~ Article is listed as a stub-class and high-importance via Molecular and Cellular Biology
~ Listed are a few recommendations by other Wikipedian, one being add a journal article to add substance to the article
~ A lead section is present but expansion is needed
~ More supportive material could be implemented to support lead section
~ Needs images and pictures (only one is present on the page currently) to support topic
~ References are present in article but many more could be added
~ Inline citations are in article

References

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  1. ^ Watson, James D.; et al. (2008). Molecular Biology of the Gene (6th ed.). San Francisco, Calif.: Benjamin Cummings. p. 157-191. ISBN 978-0-8053-9592-1.
  2. ^ Lodish, Harvey; et al. (2012). Molecular Cell Biology (7th ed.). New York: W.H. Freeman and Co. p. 256-260. ISBN 978-1-4292-3413-9.
  3. ^ Cox, Michael M.; David L. Nelson (2008). Lehninger Principles of Biochemistry (5th ed.). New York: W.H. Freeman. p. 962-969. ISBN 978-0-7167-7108-1.
  4. ^ Schuster, Stefan (July 2001). "What can silent mutations tell us?". Trends in Biotechnology. 19 (7): 242. doi:10.1016/S0167-7799(01)01695-X.
  5. ^ Hurst, Laurence D. (March 2011). "Molecular genetics: The sound of silence". Nature. 471 (7340): 582–583. doi:10.1038/471582a.
  6. ^ Moure R, Martín R, Alcaide F (October 2011). "Silent mutation in rpoB detected from clinical samples with rifampin-susceptible Mycobacterium tuberculosis". Journal of Clinical Microbiology. 49 (10): 3722. doi:10.1128/JCM.05314-11. PMC 3187328. PMID 21960703.


Initial article assessments from jakodak

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Immunolabeling[1][2][3]

- This article is a stub. It is currently a very rough list of possible applications for immunolabeling. This article needs structure, headings and sub-headings, first and foremost.
- The mechanics of immunolabeling need to be described in much greater detail.
- The diagrams require explanation and expansion.
- The main applications should receive their own subheading.
- Citations are lacking.
- There has been very little talk regarding the page, it has been ignored since April.


Cell-cell interaction[3][2]

- This article has barely begun. It consists of three sentences and one citation.
- The potential here is great as the topic is broad and categorization of the different interactions should prove to be both fun and a challenge.
- Once the categories are established, filling them in with authoritative information (many texts and reviews cover this topic very well)will be a long but simple task.
- The potential visual content is extraordinary: diagrams, artist renderings, micrographs, etc. These could greatly enhance interest in the subject matter.

Notes

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  1. ^ Lehninger
  2. ^ a b Watson
  3. ^ a b Lodish

Article Selection Rationale

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Group 81B chose to develop the article Immunolabeling. Immunolabeling is a ubiquitous technology employed by nearly all disciplines within the biological sciences. It can be a very specific means of identifying both the number and location of specific biomolecules. Since this tool is powerful, practical, and broad in scope, it should be fully explained in the world’s largest online encyclopedia.

To augment the article available, we will provide additional information via real cellular images, sample diagrams, other visual representations, various areas of science that could leverage this technique, and further organizational detail. In researching the current article, only limited visuals and inherent benefits of the technique were available. These additional details will facilitate further understanding of how this technique is executed and the intrinsic benefits of using this technology. Furthermore, we will prioritize the general discussion to offer the most relevant applications of immunolabeling.

Although the lead section has some explanation compared to some of the other article choices, its accreditation is severely lacking. Specifically, we will incorporate inline citations and references for the lead section and additional sections. We want to give the audience a sense of confidence and confirmation with the supplement of several references when necessary.

Since it is a technique and not merely a scientific phenomenon or construct, immunolabeling presents another interesting challenge; it requires a discussion regarding applicability. The advantages and disadvantages of using the technique in specific situations should be addressed in detail, providing real guidance to the reader of what the methodology entails and when other procedures would prove more useful. Although the challenge is daunting, the resources at our group’s disposal are more than sufficient to handle the task. Immunolabeling has been used for many decades and these procedures have been reduced to detailed and well known instruction sets.

Unit 9 Progress Report

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  • Lead section was substantially edited and new additions were incorporated into this section in comparison to previous lead section.
  • More references will be added to the lead section as this project continues. Since more time will be allowed, we will include sufficient references to this large lead section.
  • The cartoon in the lead section was created via Dia software.
  • Due to the lack of images that were free of copyrights on our topic, permission was granted by Dr. Thomas Caceci, Director of the Virginia-Maryland Regional College of Veterinary Medicine Morphology Research Lab, to use his immunolabeled images. Possible usage of these images may take place.

Final Progress Report

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  • The lead section was substantially reduced, which was suggested by many of the peer-reviewers and OAs.
  • The DPL bot program informed our group of some linked words that were causing our article to have disambiguation pages. Keilana explained the purpose of the program, and some words in the article were pipe linked to the correct pages.
  • Outside users, Giraffedata and Ego White Tray, clarified the usage composed vs. comprised on Wikipedia and in our article. They recommended composed should be used instead, and we agreed on their edit.
  • The article was changed from a stub to start by the user Dawynn.
  • All of our peer-reviewers, OAs, and outside editors gave us excellent suggestions and were continually supportive throughout this project. We sincerely appreciated their help.