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Protein-coding gene in the species Homo sapiens
NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene .[ 5] [ 6]
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation . Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes , or E1s, ubiquitin-conjugating enzymes , or E2s, and ubiquitin-protein ligases , or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein , NEDD8 , which can be conjugated to cellular proteins, such as Cdc53/culin.[ 6]
UBE2M has been shown to interact with NEDD8 ,[ 7] PRKAR1A [ 8] UBA3 .[ 9]
^ a b c GRCh38: Ensembl release 89: ENSG00000130725 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000005575 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S (Aug 1998). "A new NEDD8-ligating system for cullin-4A" . Genes Dev . 12 (15): 2263–8. doi :10.1101/gad.12.15.2263 . PMC 317039 PMID 9694792 . ^ a b "Entrez Gene: UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)" .^ Gong L, Yeh ET (Apr 1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway" . J. Biol. Chem . 274 (17). UNITED STATES: 12036–42. doi :10.1074/jbc.274.17.12036 ISSN 0021-9258 . PMID 10207026 . ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry" . Mol. Syst. Biol . 3 (1). England: 89. doi :10.1038/msb4100134 . PMC 1847948 PMID 17353931 . ^ Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA (February 2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1" . Mol. Cell . 17 (3): 341–50. doi :10.1016/j.molcel.2004.12.020 PMID 15694336 .
Gong L, Yeh ET (1999). "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway" . J. Biol. Chem . 274 (17): 12036–42. doi :10.1074/jbc.274.17.12036 PMID 10207026 . Chen Y, McPhie DL, Hirschberg J, Neve RL (2000). "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons" . J. Biol. Chem . 275 (12): 8929–35. doi :10.1074/jbc.275.12.8929 PMID 10722740 . Wada H, Yeh ET, Kamitani T (2000). "A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo" . J. Biol. Chem . 275 (22): 17008–15. doi :10.1074/jbc.275.22.17008 PMID 10828074 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Tomsig JL, Snyder SL, Creutz CE (2003). "Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif" . J. Biol. Chem . 278 (12): 10048–54. doi :10.1074/jbc.M212632200 PMID 12522145 . Huang DT, Miller DW, Mathew R, et al. (2004). "A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8" . Nat. Struct. Mol. Biol . 11 (10): 927–35. doi :10.1038/nsmb826 . PMC 2862556 PMID 15361859 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Huang DT, Paydar A, Zhuang M, et al. (2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1" . Mol. Cell . 17 (3): 341–50. doi :10.1016/j.molcel.2004.12.020 PMID 15694336 . Li T, Santockyte R, Shen RF, et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers" . Arch. Biochem. Biophys . 453 (1): 70–4. doi :10.1016/j.abb.2006.03.002 . PMID 16620772 . Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry" . Mol. Syst. Biol . 3 (1): 89. doi :10.1038/msb4100134 . PMC 1847948 PMID 17353931 .
PDB gallery
1y8x : Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1
2nvu : Structure of APPBP1-UBA3~NEDD8-NEDD8-MgATP-Ubc12(C111A), a trapped ubiquitin-like protein activation complex
