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The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.<ref>[http://www.medicago.se/sites/default/files/pdf/productsheets/TBS_Tween_v.01.pdf Medicago AB, (2010) Tris buffered saline with Tween pH 7.6 specification sheet]</ref> |
The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.<ref>[http://www.medicago.se/sites/default/files/pdf/productsheets/TBS_Tween_v.01.pdf Medicago AB, (2010) Tris buffered saline with Tween pH 7.6 specification sheet]</ref> |
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Tween for life yo. |
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==References== |
==References== |
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Revision as of 19:36, 21 November 2013
 | This article needs additional citations for verification. (March 2009) |
 | This article provides insufficient context for those unfamiliar with the subject. (October 2009) |
TBST (or TTBS) is mixture of Tris-Buffered Saline and Tween 20. It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays.
Contents of TBS-Tween
The following is a sample recipe for TBST:
Adjust pH with HCl to pH 7.6
The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.[1]
Tween for life yo.
References
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