PtK2 cells
PtK2 cells are a cell line derived from male long-nosed potoroo (Potorous tridactylis) epithelial kidney cells.[1] This cell line is used for a variety of applications in biomedical research but is particularly popular as a model for mitosis.[2]
Origin
[edit]The PtK2 cell line was established by Kirsten Walen and Spencer Brown in 1962.[3] Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope; mitosis becomes easy to observe, and the structural aspects of cell division can be closely examined.[4]
Characteristics
[edit]PtK2 cells are relatively large, and when grown in a monolayer, stay flat throughout the cell cycle - unlike many cells that round up during mitosis.[5] PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain).[6] PtK2 cells contain intermediate filaments composed of Keratin.[7]
References
[edit]- ^ "PtK2 (NBL-5) (ATCC® CCL-56™)". Retrieved 17 December 2013.
- ^ Elgin, edited by Barbara A. Hamkalo, Sarah C.R. (1991). Functional organization of the nucleus a laboratory guide. San Diego: Academic Press. p. 143. ISBN 008085933X.
{{cite book}}:|first=has generic name (help)CS1 maint: multiple names: authors list (link) - ^ Walen, KH; Brown, SW (Apr 28, 1962). "Chromosomes in a marsupial (Potorous tridactylis) tissue culture". Nature. 194 (4826): 406. Bibcode:1962Natur.194..406W. doi:10.1038/194406a0. PMID 14004541. S2CID 4150008.
- ^ Brescia, Peter J. Jr; Banks, Peter (24 June 2014). "High Resolution Fluorescence Microscopy of PtK2 Cells Undergoing Mitosis in Microplates". BioTek. Retrieved 20 June 2018.
- ^ "Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Male Rat Kangaroo Kidney Epithelial Cells (PtK2)". Retrieved 17 December 2013.
- ^ Steubing, Rosemarie; Lindl, Toni (25 March 2013). Atlas of Living Cell Cultures. Weinheim: Wiley. p. 465. ISBN 978-3527669936.
- ^ "Olympus Microscopy Resource Center | Fluorescence Digital Image Gallery - Epithelial Cells". Retrieved 17 December 2013.
External links
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