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P2Y receptor

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Purinergic signalling
Simplified illustration of extracellular purinergic signalling
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Purinergic signalling

Membrane transporters

Nucleoside transporters

P2Y receptors are a family of purinergic G protein-coupled receptors, stimulated by nucleotides such as adenosine triphosphate, adenosine diphosphate, uridine triphosphate, uridine diphosphate and UDP-glucose.To date, 8 P2Y receptors have been cloned in humans: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13 and P2Y14.[1]

P2Y receptors are present in almost all human tissues where they exert various biological functions based on their G-protein coupling. P2Y receptors mediate responses including vasodilation,[2] blood clotting,[3] and immune response.[4] Due to their ubiquity and variety in function, they are a common biological target in pharmacological development.[3]

Structure

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Main article: G protein-coupled receptor § Receptor Structure
P2Y12 structure as generated by PYMOL with color-coded helices

P2Y receptors are membrane proteins belonging to the class A family of G protein-coupled receptors (GPCRs).[5][6] P2Y receptor proteins display large-scale structural domains typical of GPCRs, consisting of seven hydrophobic transmembrane helices connected by three short extracellular loops and three variably sized intracellular loops; an extracellular N-terminus; and an intracellular C-terminus.[7] The extracellular regions interact with the receptor ligands, while the intracellular regions activate the G protein, control receptor internalization, and mediate dimerization.[6] Similar to other GPCRs, P2Y receptors can form both homodimers and heterodimers. These dimeric forms may vary significantly in their biochemical and pharmacological properties from the monomeric receptor.

In addition to the structural domains typical of all GPCRs, some structural elements are common across P2Y receptor subtypes. All P2Y receptors contain four extracellular cysteine residues which can form two disulfide bridges, one between the N-terminus domain and the proximal extracellular loop and another between the two remaining extracellular loops.[6] These disulfide bonds have been shown to be involved in ligand binding and signal transduction.[8] In addition, several polar residues found within the transmembrane helices are highly conserved across both species and receptor subtypes. Mutational analysis has suggested that these residues are integral to the ligand-binding mechanism of P2Y receptors. Outside of these conserved regions, the P2Y receptor family exhibits unusually high diversity in primary structure, with P2Y1 sharing only 19% of its primary structure with P2Y12.[6] Despite this, the individual P2Y subtypes are highly conserved across species, with human and mouse P2Y receptors sharing 95% of amino acids.

The ligand-binding mechanisms of P2Y receptors are not currently well established.[8] The binding complex of P2Y receptors with ATP is of significant interest, as no P2Y receptor contains amino acids sequences similar to any of the many established ATP-binding sites.[7] Recent x-ray crystallography of the human P2Y12 receptor has shown several structural irregularities in regions that are typically highly conserved across GPCRs.[8]

In contrast to the unusual structure and behavior of the extracellular ligand binding domains, P2Y intracellular domains appear to be structurally and mechanistically similar to other GPCRs.[6]

Signal transduction

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P2Y receptors respond either positively or negatively to the presence of nucleotides in extracellular solution.[9] Nucleotides may be divided into two categories: purines and pyrimidines. Individual P2Y receptor species may respond to only purines, only pyrimidines, or both; the activation profiles of the eight known P2Y receptors are listed below.[9]

P2Y species Receptivity
P2Y1 Activation by purines[9]
P2Y2 Activation by purines and pyrimidines triphosphates[9]
P2Y4 Activation by pyrimidines[9]
P2Y6 Activation by pyrimidines[9]
P2Y11 Activation by purines[9]
P2Y12 Inactivation by ADP through G1 protein[9]
P2Y13 Inactivation by ADP through G1 protein[9]
P2Y14 Activation by UDP-Glucose[9]

The activity of P2Y receptors is linked to a signal cascade originating in regulation of the flow of Ca2+ and K+ ions by the receptor's interactions with G proteins, modulating access to Ca2+ and K+ channels, though the exact behavior is dependent upon individual receptor species.[10] Voltage-independent Ca2+ channels allow for the free flow of Ca2+ ions from the cell activated by P2Y receptors.[10] Oscillation of Ca2+ concentration is directly affected by the signal-transduction activity of P2Y1; specifically, through protein kinase C phosphorylation of Thr339 in the carboxy terminus of the P2Y1 receptor.[10]

Changes in the concentration of Ca2+ have many important ramifications for the cell, including regulation of cell metabolism (e.g. autophagy initiation / regulation), ATP production (through Ca2+ entering the mitochondrial outer mitochondrial membrane and stimulation of mitochondrial dehydrogenases leading to the production of ATP), and the possibility of triggering apoptosis.[11][12] Both autophagy and apoptosis are cell stress responses that play significant roles in cells' overall life cycles, though autophagy seeks to preserve the viability of the cell by recycling unit parts of organelles, while apoptosis acts in the interest of the whole organism at the expense of the cell undergoing apoptosis.[12]

Pharmacology

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Clopidogrel (Plavix), an inhibitor of the P2Y12 receptor, was formerly the second best-selling drug in the world[13]

Many commonly prescribed medications target P2Y receptors, and active research is being conducted into developing new drugs targeting these receptors.[3] The most commonly prescribed drug targeting P2Y receptors is clopidogrel, an antiplatelet medication which acts on the P2Y12 receptor in a manner shared with other thienopyridines.[14] Other pharmaceutical applications include denufosol, which targets P2Y2 and is being investigated for the treatment of cystic fibrosis, and diquafosol, a P2Y2 agonist used in the treatment of dry eye disease.[15][16][17]

P2Y6 receptors have been shown to play a role in cerebral vasodilation. UDP-analogs which bind to this receptor have been investigated as possible treatments for migraines.[18][17]

P2Y11 is a regulator of immune response, and a common polymorphism carried by almost 20% of North European caucasians give increased risk of myocardial infarction, making P2Y11 an interesting drug target candidate for treatment of myocardial infarction.[4][16][17]

In addition to established uses, pharmaceutical research has been conducted into the role of P2Y receptors in osteoporosis,[2] diabetes,[19] and cardio-protection.[20][17]

Coupling

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The biological effects of P2Y receptor activation depends on how they couple to downstream signalling pathways, either via Gi, Gq/11 or Gs G proteins. Human P2Y receptors have the following G protein coupling:[21]

Protein Gene Coupling Nucleotide
P2RY1 P2RY1 Gq/11 ADP
P2RY2 P2RY2 Gq/11 (and Gi) ATP, UTP
P2RY4 P2RY4 Gq/11 (and Gi) UTP
P2RY5 / LPA6 LPAR6 Lysophosphatidic acid[22]
P2RY6 P2RY6 Gq/11 UDP
P2RY8 P2RY8 orphan receptor
P2RY9 / LPAR4 / GPR23 LPAR4 Lysophosphatidic acid
P2RY10 P2RY10 orphan receptor
P2RY11 P2RY11 Gq/11 and Gs ATP
P2RY12 P2RY12 Gi ADP
P2RY13 P2RY13 Gi ADP
P2RY14 P2RY14 Gi UDP-glucose

The gaps in P2Y receptor numbering is due to that several receptors (P2Y3, P2Y5, P2Y7, P2Y8, P2Y9, P2Y10) were thought to be P2Y receptors when they were cloned, when in fact they are not.

See also

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References

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  1. ^ Abbracchio MP, Burnstock G, Boeynaems JM, Barnard EA, Boyer JL, Kennedy C, Knight GE, Fumagalli M, Gachet C, Jacobson KA, Weisman GA (September 2006). "International Union of Pharmacology LVIII: update on the P2Y G protein-coupled nucleotide receptors: from molecular mechanisms and pathophysiology to therapy". Pharmacological Reviews. 58 (3): 281–341. doi:10.1124/pr.58.3.3. PMC 3471216. PMID 16968944.
  2. ^ a b Romanello M, Bivi N, Pines A, Deganuto M, Quadrifoglio F, Moro L, Tell G (October 2006). "Bisphosphonates activate nucleotide receptors signaling and induce the expression of Hsp90 in osteoblast-like cell lines". Bone. 39 (4): 739–53. doi:10.1016/j.bone.2006.03.011. PMID 16697713.
  3. ^ a b c Erlinge D (2011-01-01). "P2Y receptors in health and disease". Pharmacology of Purine and Pyrimidine Receptors. Advances in Pharmacology. Vol. 61. pp. 417–39. doi:10.1016/B978-0-12-385526-8.00013-8. ISBN 9780123855268. PMID 21586366.
  4. ^ a b Amisten S, Melander O, Wihlborg AK, Berglund G, Erlinge D (January 2007). "Increased risk of acute myocardial infarction and elevated levels of C-reactive protein in carriers of the Thr-87 variant of the ATP receptor P2Y11". European Heart Journal. 28 (1): 13–8. doi:10.1093/eurheartj/ehl410. PMID 17135283.
  5. ^ Jacobson KA, Jayasekara MP, Costanzi S (September 2012). "Molecular Structure of P2Y Receptors: Mutagenesis, Modeling, and Chemical Probes". Wiley Interdisciplinary Reviews: Membrane Transport and Signaling. 1 (6): 815–827. doi:10.1002/wmts.68. PMC 3547624. PMID 23336097.
  6. ^ a b c d e von Kügelgen I, Harden TK (2011). "Chapter 12: Molecular Pharmacology, Physiology, and Structure of the P2Y Receptors". In Jacobson KA, Linden J (eds.). Pharmacology of Purine and Pyrimidine Receptors. Elsevier. pp. 373–399. ISBN 978-0-12-385526-8. Retrieved 8 November 2018.
  7. ^ a b Dubyak, George R. (2013). "P2Y Receptors". Encyclopedia of Biological Chemistry (2nd. ed.). Cleveland, OH: Elsevier Inc. pp. 375–378. doi:10.1016/B978-0-12-378630-2.00350-9. ISBN 978-0-12-378631-9.
  8. ^ a b c Zhang K, Zhang J, Gao ZG, Zhang D, Zhu L, Han GW, et al. (May 2014). "Structure of the human P2Y12 receptor in complex with an antithrombotic drug". Nature. 509 (7498): 115–8. Bibcode:2014Natur.509..115Z. doi:10.1038/nature13083. PMC 4174307. PMID 24670650.
  9. ^ a b c d e f g h i j Tulapurkar ME, Schäfer R, Hanck T, Flores RV, Weisman GA, González FA, Reiser G (June 2005). "Endocytosis mechanism of P2Y2 nucleotide receptor tagged with green fluorescent protein: clathrin and actin cytoskeleton dependence". Cellular and Molecular Life Sciences. 62 (12): 1388–99. doi:10.1007/s00018-005-5052-0. PMC 11139135. PMID 15924261.
  10. ^ a b c Van Kolen K, Slegers H (September 2006). "Integration of P2Y receptor-activated signal transduction pathways in G protein-dependent signalling networks". Purinergic Signalling. 2 (3): 451–69. doi:10.1007/s11302-006-9008-0. PMC 2254474. PMID 18404483.
  11. ^ Hajnóczky G, Csordás G, Das S, Garcia-Perez C, Saotome M, Sinha Roy S, Yi M (2006-11-01). "Mitochondrial calcium signalling and cell death: approaches for assessing the role of mitochondrial Ca2+ uptake in apoptosis". Cell Calcium. 40 (5–6): 553–60. doi:10.1016/j.ceca.2006.08.016. PMC 2692319. PMID 17074387.
  12. ^ a b Decuypere JP, Bultynck G, Parys JB (September 2011). "A dual role for Ca(2+) in autophagy regulation". Cell Calcium. 50 (3): 242–50. doi:10.1016/j.ceca.2011.04.001. PMID 21571367.
  13. ^ Doll J, Zeitler E, Becker R (July 2013). "Generic clopidogrel: time to substitute?". JAMA. 310 (2): 145–6. doi:10.1001/jama.2013.7155. PMID 23839745.
  14. ^ von Kügelgen I (2017). 12 Receptor. Advances in Experimental Medicine and Biology. Vol. 1051. pp. 123–138. doi:10.1007/5584_2017_98. ISBN 978-981-10-7610-7. PMID 28921447.
  15. ^ Peral A, Domínguez-Godínez CO, Carracedo G, Pintor J (April 2008). "Therapeutic targets in dry eye syndrome". Drug News & Perspectives. 21 (3): 166–76. PMID 18560615.
  16. ^ a b Wan HX, Hu JH, Xie R, Yang SM, Dong H (May 2016). "Important roles of P2Y receptors in the inflammation and cancer of digestive system". Oncotarget. 7 (19): 28736–47. doi:10.18632/oncotarget.7518. PMC 5053759. PMID 26908460.
  17. ^ a b c d von Kügelgen I, Hoffmann K (May 2016). "Pharmacology and structure of P2Y receptors". Neuropharmacology. 104: 50–61. doi:10.1016/j.neuropharm.2015.10.030. PMID 26519900.
  18. ^ Malmsjö M, Hou M, Pendergast W, Erlinge D, Edvinsson L (May 2003). "Potent P2Y6 receptor mediated contractions in human cerebral arteries". BMC Pharmacology. 3 (1): 4. doi:10.1186/1471-2210-3-4. PMC 156657. PMID 12737633.
  19. ^ Amisten S, Meidute-Abaraviciene S, Tan C, Olde B, Lundquist I, Salehi A, Erlinge D (September 2010). "ADP mediates inhibition of insulin secretion by activation of P2Y13 receptors in mice". Diabetologia. 53 (9): 1927–34. doi:10.1007/s00125-010-1807-8. PMID 20526761.
  20. ^ Yitzhaki S, Shainberg A, Cheporko Y, Vidne BA, Sagie A, Jacobson KA, Hochhauser E (October 2006). "Uridine-5'-triphosphate (UTP) reduces infarct size and improves rat heart function after myocardial infarct". Biochemical Pharmacology. 72 (8): 949–55. doi:10.1016/j.bcp.2006.07.019. PMC 4429760. PMID 16939682.
  21. ^ HUGO Gene Nomenclature Committee, Gene Family: Purinergic receptors P2Y (P2RY), retrieved 2017-01-26.
  22. ^ Pasternack SM, von Kügelgen I, Aboud KA, Lee YA, Rüschendorf F, Voss K, Hillmer AM, Molderings GJ, Franz T, Ramirez A, Nürnberg P, Nöthen MM, Betz RC (March 2008). "G protein-coupled receptor P2Y5 and its ligand LPA are involved in maintenance of human hair growth". Nature Genetics. 40 (3): 329–34. doi:10.1038/ng.84. PMID 18297070.
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