Louis Pasteur

Louis Pasteur
French microbiologist and chemist
Born	(1822-12-27)December 27, 1822 Dole, Jura, Franche-Comté, France
Died	September 28, 1895(1895-09-28) (aged 72) Marnes-la-Coquette, Hauts-de-Seine, France
Nationality	French
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Louis Pasteur (pronounced [pastøʁ] December 27, 1822 – September 28, 1895) was a French chemist and microbiologist born in Dole. He is best known for his remarkable breakthroughs in the causes and preventions of disease. His discoveries reduced mortality from puerperal fever, and he created the first vaccine for rabies. His experiments supported the germ theory of disease. He was best known to the general public for inventing a method to stop milk and wine from causing sickness, a process that came to be called pasteurization. He is regarded as one of the three main founders of microbiology, together with Ferdinand Cohn and Robert Koch. Pasteur also made many discoveries in the field of chemistry, most notably the molecular basis for the asymmetry of certain crystals. His body lies beneath the Institute Pasteur in Paris in a spectacular vault covered in depictions of his accomplishments in Byzantine mosaics.

Early life and biography

Louis Pasteur was born on December 27, 1822, in Dole in the Jura region of France into the family of a poor tanner. He grew up in the nearby town of Arbois. There, he later had his house and laboratory, which is now a Pasteur museum. He gained degrees in Letters and in Mathematical Sciences before entering the École Normale Supérieure, the college for teachers. After serving briefly as professor of physics at Dijon Lycée in 1848, he became professor of chemistry at the University of Strasbourg, where he met and courted Marie Laurent, daughter of the university's rector, in 1849. They were married on May 29, 1849, and together they had five children, only two of whom survived to adulthood; three of them died, two of typhoid and one of a brain tumor. These personal tragedies inspired Pasteur to try to find cures for diseases such as typhoid.

Work on chirality and the polarization of light

Pasteur separated the left and right crystal shapes from each other to form two piles of crystals: in solution one form rotated light to the left, the other to the right, while an equal mixture of the two forms canceled each other's effect and does not rotate polarized light.

In Pasteur's early work as a chemist, he resolved a problem concerning the nature of tartaric acid (1849). A solution of this compound derived from living things (specifically, wine lees) rotated the plane of polarization of light passing through it. The mystery was that tartaric acid derived by chemical synthesis had no such effect, even though its chemical reactions were identical and its elemental composition was the same.

Upon examination of the minuscule crystals of sodium ammonium tartrate, Pasteur noticed that the crystals came in two asymmetric forms that were mirror images of one another. Tediously sorting the crystals by hand gave two forms of the compound: solutions of one form rotated polarized light clockwise, while the other form rotated light counterclockwise. An equal mix of the two had no polarizing effect on light. Pasteur correctly deduced the molecule in question was asymmetric and could exist in two different forms that resemble one another as would left- and right-hand gloves, and that the biological source of the compound provided purely the one type. This was the first time anyone had demonstrated chiral molecules.

Pasteur's doctoral thesis on crystallography attracted the attention of M. Puillet and he helped Pasteur garner a position of professor of chemistry at the Faculté (College) of Strasbourg.

In 1854, he was named Dean of the new Faculty of Sciences in Lille. In 1856, he was made administrator and director of scientific studies of the École Normale Supérieure.

Germ theory

Pasteur demonstrated that fermentation is caused by the growth of microorganisms, and that the emergent growth of bacterium in nutrient broths is not due to spontaneous generation but rather to biogenesis (Omne vivum ex ovo).

Bottle en col de cygne used by Pasteur

Institut Pasteur de Lille

He exposed boiled broths to air in vessels that contained a filter to prevent all particles from passing through to the growth medium, and even in vessels with no filter at all, with air being admitted via a long tortuous tube that would not allow dust particles to pass. Nothing grew in the broths unless the flasks were broken open; therefore, the living organisms that grew in such broths came from outside, as spores on dust, rather than spontaneously generated within the broth. This was one of the last and most important experiments disproving the theory of spontaneous generation. The experiment also supported germ theory.

While Pasteur was not the first to propose germ theory (Girolamo Fracastoro, Agostino Bassi, Friedrich Henle and others had suggested it earlier), he developed it and conducted experiments that clearly indicated its correctness and managed to convince most of Europe it was true. Today he is often regarded as the father of germ theory and bacteriology, together with Robert Koch.

Pasteur's research also showed that the growth of microorganisms was responsible for spoiling beverages, such as beer, wine and milk. With this established, he invented a process in which liquids such as milk were heated to kill most bacteria and molds already present within them. He and Claude Bernard completed the first test on April 20, 1862. This process was soon afterwards known as pasteurization.

Beverage contamination led Pasteur to the idea that microorganisms infecting animals and humans cause disease. He proposed preventing the entry of microorganisms into the human body, leading Joseph Lister to develop antiseptic methods in surgery.

In 1865, two parasitic diseases called pébrine and flacherie were killing great numbers of silkworms at Alais (now Alès). Pasteur worked several years proving it was a microbe attacking silkworm eggs which caused the disease, and that eliminating this microbe within silkworm nurseries would eradicate the disease.

Pasteur also discovered anaerobiosis, whereby some microorganisms can develop and live without air or oxygen, called the Pasteur effect.

Immunology and vaccination

Pasteur's later work on diseases included work on chicken cholera. During this work, a culture of the responsible bacteria had spoiled and failed to induce the disease in some chickens he was infecting with the disease. Upon reusing these healthy chickens, Pasteur discovered that he could not infect them, even with fresh bacteria; the weakened bacteria had caused the chickens to become immune to the disease, even though they had only caused mild symptoms.

His assistant Charles Chamberland (of French origin) had been instructed to inoculate the chickens after Pasteur went on holiday. Chamberland failed to do this, but instead went on holiday himself. On his return, the month old cultures made the chickens unwell, but instead of the infection being fatal, as it usually was, the chickens recovered completely. Chamberland assumed an error had been made, and wanted to discard the apparently faulty culture when Pasteur stopped him. Pasteur guessed the recovered animals now might be immune to the disease, as were the animals at Eure-et-Loir that had recovered from anthrax.

In the 1870s, he applied this immunisation method to anthrax, which affected cattle, and aroused interest in combating other diseases.

Louis Pasteur in his laboratory, painting by A. Edelfeldt in 1885

Pasteur publicly claimed he had made the anthrax vaccine by exposing the bacillus to oxygen. His laboratory notebooks, now in the Bibliotheque Nationale in Paris, in fact show Pasteur used the method of rival Jean-Joseph-Henri Toussaint, a Toulouse veterinary surgeon, to create the anthrax vaccine. This method used the oxidizing agent potassium dichromate. Pasteur's oxygen method did eventually produce a vaccine but only after he had been awarded a patent on the production of an anthrax vaccine.

The notion of a weak form of a disease causing immunity to the virulent version was not new; this had been known for a long time for smallpox. Inoculation with smallpox was known to result in far less scarring, and greatly reduced mortality, in comparison to the naturally acquired disease. Edward Jenner had also discovered vaccination, using cowpox to give cross-immunity to smallpox (in 1796), and by Pasteur's time this had generally replaced the use of actual smallpox material in inoculation. The difference between smallpox vaccination and cholera and anthrax vaccination was that the weakened form of the latter two disease organisms had been generated artificially, and so a naturally weak form of the disease organism did not need to be found.

This discovery revolutionized work in infectious diseases, and Pasteur gave these artificially weakened diseases the generic name of vaccines, to honour Jenner's discovery. Pasteur produced the first vaccine for rabies by growing the virus in rabbits, and then weakening it by drying the affected nerve tissue.

The rabies vaccine was initially created by Emile Roux, a French doctor and a colleague of Pasteur who had been working with a killed vaccine produced by desiccating the spinal cords of infected rabbits. The vaccine had only been tested on eleven dogs before its first human trial.

This vaccine was first used on 9-year old Joseph Meister, on July 6, 1885, after the boy was badly mauled by a rabid dog. This was done at some personal risk for Pasteur, since he was not a licensed physician and could have faced prosecution for treating the boy. However, left without treatment, the boy faced almost certain death from rabies. After consulting with colleagues, Pasteur decided to go ahead with the treatment. The treatment proved to be a spectacular success, with Meister avoiding the disease; thus, Pasteur was hailed as a hero and the legal matter was not pursued. The treatment's success laid the foundations for the manufacture of many other vaccines. The first of the Pasteur Institutes was also built on the basis of this achievement.

Legal risk was not the only kind Pasteur undertook. In The Story of San Michele, Axel Munthe writes of the rabies vaccine research:

Pasteur himself was absolutely fearless. Anxious to secure a sample of saliva straight from the jaws of a rabid dog, I once saw him with the glass tube held between his lips draw a few drops of the deadly saliva from the mouth of a rabid bull-dog, held on the table by two assistants, their hands protected by leather gloves.

Louis Pasteur portrait in his later years.

Because of his study in germs, Pasteur encouraged doctors to sanitize their hands and equipment before surgery. Prior to this, few doctors or their assistants practiced the procedure of washing their hands and equipment.

Allegations of deception

In 1995, the centennial of the death of Louis Pasteur, the New York Times ran an article titled "Pasteur's Deception". After having thoroughly read Pasteur's lab notes the science historian Gerald L. Geison declared that Pasteur had given a misleading account of the preparation of the anthrax vaccine used in the experiment at Pouilly-le-Fort.

Honours and final days

His death occurred in 1895, near Paris, from complications of a series of strokes that had started in 1868. He died while listening to the story of St Vincent de Paul, whom he admired and sought to emulate. He was buried in the Cathedral of Notre Dame, but his remains were reinterred in a crypt in the Institut Pasteur, Paris, where he is remembered for his life-saving work.

Pasteur won the Leeuwenhoek medal, microbiology's highest Dutch honor in Arts and Sciences, in 1895 his dad died and his mom got raped. He was a Grand Croix of the Legion of Honor–one of only 75 in all of France. Both Institute Pasteur and Université Louis Pasteur were named after him.

In many localities worldwide, there are streets named in his honour. For example, in the USA: the Medical school at Stanford University, Palo Alto and Irvine, California, Boston, Massachusetts and Polk, Florida; Jonquière, Québec; San Salvador de Jujuy (Argentina), Yarmouth and Norfolk in the United Kingdom, Jericho and Wulguru in Queensland, (Australia); Phnom Penh in Cambodia; Ho Chi Minh City in Viet Nam; Batna in Algeria, Tehran in Iran, Milan in Italy and Bucharest in Romania.

Pasteur was ranked #12 in the 1978 edition of Michael H. Hart's controversial book, The 100: A Ranking Of The Most Influential Persons in History. However, Pasteur was promoted to no. 11, replacing Karl Marx in the 1992 revised edition of the book.

Statements

In his triumphal lecture at the Sorbonne in 1864, Pasteur said "Never will the doctrine of spontaneous generation recover from the mortal blow struck by this simple experiment" (referring to his swan-neck flask experiment wherein he proved that fermenting microorganisms would not form in a flask containing fermentable juice until an entry path was created for them).

See also

• Infection control
• Infectious disease
• Modern medicine
• Pasteur Institute
• The Story of Louis Pasteur (a 1936 biographical film).

References

Biographies

• Debré, P. (1998). Louis Pasteur. Baltimore, MD: Johns Hopkins University Press. ISBN 0-8018-5808-9. {{cite book}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
• Geison, Gerald L. (1995). The private science of Louis Pasteur. Princeton, NJ: Princeton University Press. ISBN 0-691-03442-7.
• Tiner, John Hudson (1990). Louis Pasteur: Founder of Modern Medicine. Fenton, MI: Mott Media. ISBN 0-88062-159-1.
• Herbermann, Charles, ed. (1913). "Louis Pasteur" . Catholic Encyclopedia. New York: Robert Appleton Company.

Influence on medicine and society

• Latour, Bruno (1988). The Pasteurization of France. Boston: Harvard University Press. ISBN 0-674-65761-6.
• Ullmann, Agnes (2007). "Pasteur–Koch: Distinctive Ways of Thinking about Infectious Diseases" (PDF). Microbe. 2 (8). American Society for Microbiology: 383–387. Retrieved 2007-12-02.
• Miller, George (1901). A Text-book of Bacteriology. Wood. pp. pp.278–279. Retrieved 2007-12-02. {{cite book}}: |pages= has extra text (help)

Footnotes

External links

• The Institut Pasteur - Foundation Dedicated to the prevention and treatment of diseases through biological research, education and public health activities
• The Pasteur Foundation - A US nonprofit organization dedicated to promoting the mission of the Institut Pasteur in Paris. Full archive of newsletters available online containing examples of US Tributes to Louis Pasteur.
• Pasteur's Papers on the Germ Theory
• Pasteur's Work on Beer Brewing
• The Pasteur Galaxy
• Louis Pasteur featured on the 5 French Franc banknote from 1966.
• Germ Theory and Its Applications to Medicine and Surgery, 1878
• LIFE magazine's top 100 events of the millennium: Germ theory of disease and Pasteur
• Louis Pasteur (1822-1895) profile, AccessExcellence.org

The complete work of Pasteur, BNF (Bibliothèque nationale de France)

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• Comptes rendus de l’Académie des sciences Articles published by Pasteur Template:Fr icon

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