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{{further2|[[Genetic engineering]], Genetic [[modifications (genetics)|modification]], [[Horizontal gene transfer]], [[Molecular cloning]], [[Recombinant DNA]] and [[Transformation (genetics)]]}}
{{further2|[[Genetic engineering]], Genetic [[modifications (genetics)|modification]], [[Horizontal gene transfer]], [[Molecular cloning]], [[Recombinant DNA]] and [[Transformation (genetics)]]}}


Genetic modification involves the mutation, insertion, or deletion of genes. When genes are inserted, they usually come from a different species, which is a form of [[horizontal gene transfer]]. In nature this can occur when exogenous DNA penetrates the [[cell membrane]] for any reason. To do this artificially may require attaching the genes to a [[virus]] or just physically inserting the extra DNA into the nucleus of the intended host with a very small syringe, with the use of [[electroporation]] (that is, introducing DNA from one organism into the cell of another by use of an electric pulse) or with very small particles fired from a [[gene gun]].<ref>Cornell Chronicle, May 14, 1987, page 3.[http://www.ecommons.cornell.edu/bitstream/1813/25239/1/018_33.pdf Biologists invent gun for shooting cells with DNA]</ref><ref>Sanford JC et al (1987) [http://www.tandfonline.com/doi/abs/10.1080/02726358708904533 Delivery of substances into cells and tissues using a particle bombardment process]. Journal of Particulate Science and Technology 5:27-37.</ref><ref>Klein, TM et al (1987) [http://www.nature.com/nature/journal/v327/n6117/abs/327070a0.html High-velocity microprojectiles for delivering nucleic acids into living cells]. Nature 327:70-73.</ref> However, other methods exploit natural forms of gene transfer, such as the ability of ''[[Agrobacterium]]'' to transfer genetic material to plants,<ref>{{Cite journal
GMO stands for gay mom orgy. Eat shit mothafucka!!! Genetic modification involves the mutation, insertion, or deletion of genes. When genes are inserted, they usually come from a different species, which is a form of [[horizontal gene transfer]]. In nature this can occur when exogenous DNA penetrates the [[cell membrane]] for any reason. To do this artificially may require attaching the genes to a [[virus]] or just physically inserting the extra DNA into the nucleus of the intended host with a very small syringe, with the use of [[electroporation]] (that is, introducing DNA from one organism into the cell of another by use of an electric pulse) or with very small particles fired from a [[gene gun]].<ref>Cornell Chronicle, May 14, 1987, page 3.[http://www.ecommons.cornell.edu/bitstream/1813/25239/1/018_33.pdf Biologists invent gun for shooting cells with DNA]</ref><ref>Sanford JC et al (1987) [http://www.tandfonline.com/doi/abs/10.1080/02726358708904533 Delivery of substances into cells and tissues using a particle bombardment process]. Journal of Particulate Science and Technology 5:27-37.</ref><ref>Klein, TM et al (1987) [http://www.nature.com/nature/journal/v327/n6117/abs/327070a0.html High-velocity microprojectiles for delivering nucleic acids into living cells]. Nature 327:70-73.</ref> However, other methods exploit natural forms of gene transfer, such as the ability of ''[[Agrobacterium]]'' to transfer genetic material to plants,<ref>{{Cite journal
|author=Lee LY, Gelvin SB |title=T-DNA binary vectors and systems |journal=Plant Physiol. |volume=146 |issue=2 |pages=325–332
|author=Lee LY, Gelvin SB |title=T-DNA binary vectors and systems |journal=Plant Physiol. |volume=146 |issue=2 |pages=325–332
|date=February 2008 |pmid=18250230 |pmc=2245830 |doi=10.1104/pp.107.113001 |oclc=1642351
|date=February 2008 |pmid=18250230 |pmc=2245830 |doi=10.1104/pp.107.113001 |oclc=1642351

Revision as of 18:51, 5 March 2014

"GMO" redirects here. For other uses, see GMO (disambiguation).
For related content, see genetically modified food controversies, genetic engineering, genetically modified crops, genetically modified food, and regulation of the release of genetically modified organisms.

File:GloFish.jpg
GloFish, the first genetically modified animal to be sold as a pet

A genetically modified organism (GMO) is an organism whose genetic material has been altered using genetic engineering techniques. Organisms that have been genetically modified include micro-organisms such as bacteria and yeast, insects, plants, fish, and mammals. GMOs are the source of genetically modified foods, and are also widely used in scientific research and to produce goods other than food. The term GMO is very close to the technical legal term, 'living modified organism' defined in the Cartagena Protocol on Biosafety, which regulates international trade in living GMOs (specifically, "any living organism that possesses a novel combination of genetic material obtained through the use of modern biotechnology").

This article focuses on what organisms have been genetically engineered, and for what purposes. The article on genetic engineering focuses on the history and methods of genetic engineering, and on applications of genetic engineering and of GMOs. Both articles cover much of the same ground but with different organizations (sorted by organism in this article; sorted by application in the other). There are separate articles on genetically modified crops, genetically modified food, regulation of the release of genetic modified organisms, and controversies.

Production

Further information: Genetic engineering, Genetic modification, Horizontal gene transfer, Molecular cloning, Recombinant DNA and Transformation (genetics)

GMO stands for gay mom orgy. Eat shit mothafucka!!! Genetic modification involves the mutation, insertion, or deletion of genes. When genes are inserted, they usually come from a different species, which is a form of horizontal gene transfer. In nature this can occur when exogenous DNA penetrates the cell membrane for any reason. To do this artificially may require attaching the genes to a virus or just physically inserting the extra DNA into the nucleus of the intended host with a very small syringe, with the use of electroporation (that is, introducing DNA from one organism into the cell of another by use of an electric pulse) or with very small particles fired from a gene gun.[1][2][3] However, other methods exploit natural forms of gene transfer, such as the ability of Agrobacterium to transfer genetic material to plants,[4] or the ability of lentiviruses to transfer genes to animal cells.[5]

History

Main article: History of genetic engineering

The general principle of producing a GMO is to alter the genetic material of an organism's genome. Genetic modification caused by human activity has been occurring since humans first domesticated animals in 12 000 BC.[6]: 1  and plants around 10,000 BC.[7]: 1  Genetic engineering, the direct transfer of DNA from one organism to another, was first accomplished by Herbert Boyer and Stanley Cohen in 1973.[8] Advances have allowed scientists to manipulate and add genes to a variety of different organism and to induce a range of different effects. Since 1976 the technology has been commercialised, with companies producing and selling genetically modified food and medicine. This may involve mutating, deleting, or adding genetic material. When genetic material from a different species is added, the resulting DNA is called recombinant DNA and the organism is called a transgenic organism. The first recombinant DNA molecules were produced by Paul Berg in 1972.[9][10]

Uses

GMOs are used in biological and medical research, production of pharmaceutical drugs, experimental medicine (e.g. gene therapy), and agriculture (e.g. golden rice, resistance to herbicides). The term "genetically modified organism" does not always imply, but can include, targeted insertions of genes from one species into another. For example, a gene from a jellyfish, encoding a fluorescent protein called GFP, can be physically linked and thus co-expressed with mammalian genes to identify the location of the protein encoded by the GFP-tagged gene in the mammalian cell. Such methods are useful tools for biologists in many areas of research, including those who study the mechanisms of human and other diseases or fundamental biological processes in eukaryotic or prokaryotic cells.

Plants

Transgenic plants

Kenyans examining insect-resistant transgenic Bt corn

Transgenic plants have been engineered for scientific research, to create new colours in plants, and to create different crops.

In research, plants are engineered to help discover the functions of certain genes. One way to do this is to knock out the gene of interest and see what phenotype develops. Another strategy is to attach the gene to a strong promoter and see what happens when it is over expressed. A common technique used to find out where the gene is expressed is to attach it to GUS or a similar reporter gene that allows visualisation of the location.[11]'

Suntory "blue" rose

After thirteen years of collaborative research, an Australian company – Florigene, and a Japanese company – Suntory, created a blue rose (actually lavender or mauve) in 2004.[12] The genetic engineering involved three alterations – adding two genes, and interfering with another. One of the added genes was for the blue plant pigment delphinidin cloned from the pansy.[13] The researchers then used RNA interference (RNAi) technology to depress all color production by endogenous genes by blocking a crucial protein in color production, called dihydroflavonol 4-reductase) (DFR), and adding a variant of that protein that would not be blocked by the RNAi but that would allow the delphinidin to work.[13] The roses are sold worldwide.[14][15] Florigene has also created and sells lavender-colored carnations that are genetically engineered in a similar way.[13]

Simple plants and plant cells have been genetically engineered for production of biopharmaceuticals in bioreactors as opposed to cultivating plants in open fields. Work has been done with duckweed Lemna minor,[16] the algae Chlamydomonas reinhardtii[17] and the moss Physcomitrella patens.[18][19] An Israeli company, Protalix, has developed a method to produce therapeutics in cultured transgenic carrot and tobacco cells.[20] Protalix and its partner, Pfizer, received FDA approval to market its drug Elelyso, a treatment for Gaucher's Disease, in 2012.[21]

GM crops
Main article: Genetically modified crops

In agriculture, currently marketed genetically engineered crops have traits such as resistance to pests, resistance to herbicides, increased nutritional value, or production of valuable goods such as drugs (pharming). Products under development include crops that are able to thrive in environmental conditions outside the species' native range or in changed conditions in their range (e.g. drought or salt resistance). Products that existed and have been withdrawn include those with extended product shelf life, such as the Flavr-savr tomato.

Since the first commercial cultivation of genetically modified plants in 1996, they have been modified to be tolerant to the herbicides glufosinate and glyphosate, to be resistant to virus damage (as in Ringspot virus-resistant GM papaya grown in Hawaii), and to produce the Bt toxin, an insecticide that is documented as non-toxic to mammals.[22][23] Plants, including algae, jatropha, maize, and poplars,[24] have been genetically modified for use in producing fuel, known as biofuel.

Second- and third-generation GM crops are on the market and under development with improved nutrition profiles and increased yields or ability to thrive in difficult environments.[25] GM oilseed crops on the market today offer improved oil profiles for processing or healthier edible oils.[26] Other examples include:

For discussions of issues about GM crops and GM food, see the Controversies section below and the article on genetically modified food controversies.

Cisgenic plants

Cisgenesis, sometimes also called intragenesis, is a product designation for a category of genetically engineered plants. A variety of classification schemes have been proposed[32] that order genetically modified organisms based on the nature of introduced genotypical changes rather than the process of genetic engineering.

While some genetically modified plants are developed by the introduction of a gene originating from distant, sexually incompatible species into the host genome, cisgenic plants contain genes that have been isolated either directly from the host species or from sexually compatible species. The new genes are introduced using recombinant DNA methods and gene transfer. Some scientists hope that the approval process of cisgenic plants might be simpler than that of proper transgenics,[33] but it remains to be seen.[34]

Microbes

Bacteria were the first organisms to be modified in the laboratory, due to their simple genetics.[35] These organisms are now used for several purposes, and are particularly important in producing large amounts of pure human proteins for use in medicine.[36]

Genetically modified bacteria are used to produce the protein insulin to treat diabetes.[37] Similar bacteria have been used to produce biofuels,[38] clotting factors to treat haemophilia,[39] and human growth hormone to treat various forms of dwarfism.[40][41]

In addition, various genetically engineered micro-organisms are routinely used as sources of enzymes for the manufacture of a variety of processed foods. These include alpha-amylase from bacteria, which converts starch to simple sugars, chymosin from bacteria or fungi that clots milk protein for cheese making, and pectinesterase from fungi which improves fruit juice clarity.[42]

Mammals

Some chimeras, like the blotched mouse shown, are created through genetic modification techniques like gene targeting.

Genetically modified mammals are an important category of genetically modified organisms.[43] Ralph L. Brinster and Richard Palmiter developed the techniques responsible for transgenic mice, rats, rabbits, sheep, and pigs in the early 1980s, and established many of the first transgenic models of human disease, including the first carcinoma caused by a transgene. The process of genetically engineering animals is a slow, tedious, and expensive process. However, new technologies are making genetic modifications easier and more precise.[44]

The first transgenic (genetically modified) animal was produced by injecting DNA into mouse embryos then implanting the embryos in female mice.[45]

Genetically modified animals currently being developed can be placed into six different broad classes based on the intended purpose of the genetic modification:

  1. to research human diseases (for example, to develop animal models for these diseases);
  2. to produce industrial or consumer products (fibres for multiple uses);
  3. to produce products intended for human therapeutic use (pharmaceutical products or tissue for implantation);
  4. to enrich or enhance the animals' interactions with humans (hypo-allergenic pets);
  5. to enhance production or food quality traits (faster growing fish, pigs that digest food more efficiently);
  6. to improve animal health (disease resistance)[46]

Research use

Transgenic animals are used as experimental models to perform phenotypic and for testing in biomedical research.[47]

Genetically modified (genetically engineered) animals are becoming more vital to the discovery and development of cures and treatments for many serious diseases. By altering the DNA or transferring DNA to an animal, we can develop certain proteins that may be used in medical treatment. Stable expressions of human proteins have been developed in many animals, including sheep, pigs, and rats. Human-alpha-1-antitrypsin,[48] which has been tested in sheep and is used in treating humans with this deficiency and transgenic pigs with human-histo-compatibility have been studied in the hopes that the organs will be suitable for transplant with less chances of rejection.

Scientists have genetically engineered several organisms, including some mammals, to include green fluorescent protein (GFP) for medical research purposes (Chalfie, Shimoura, and Tsien were awarded the Nobel prize in 2008 for GFP[49]). For example fluorescent pigs have been bred in the US in 2000,[50] in Korea in 2002,[51] in Taiwan in 2006,[52] in China in 2008[53] and Japan in 2009.[54] These pigs were bred to study human organ transplants,[53] regenerating ocular photoreceptor cells,[55] neuronal cells in the brain,[55] regenerative medicine via stem cells,[56] tissue engineering,[54] and other diseases. In 2011 a Japanese-American Team created green-fluorescent cats in order to find therapies for HIV/AIDS and other diseases[57] as Feline immunodeficiency virus (FIV) is related to HIV.[58]

In 2009, scientists in Japan announced that they had successfully transferred a gene into a primate species (marmosets) and produced a stable line of breeding transgenic primates for the first time.[59][60] Their first research target for these marmosets was Parkinson's disease, but they were also considering Amyotrophic lateral sclerosis and Huntington's disease.[61]

Producing human therapeutics

Within the field known as pharming, intensive research has been conducted to develop transgenic animals that produce biotherapeutics.[62] On 6 February 2009, the U.S. Food and Drug Administration approved the first human biological drug produced from such an animal, a goat. The drug, ATryn, is an anticoagulant which reduces the probability of blood clots during surgery or childbirth. It is extracted from the goat's milk.[63]

Production or food quality traits

Enviropig was a genetically enhanced line of Yorkshire pigs in Canada created with the capability of digesting plant phosphorus more efficiently than conventional Yorkshire pigs. The project ended in 2012.[64][65] These pigs produced the enzyme phytase, which breaks down the indigestible phosphorus, in their saliva. The enzyme was introduced into the pig chromosome by pronuclear microinjection. With this enzyme, the animal is able to digest cereal grain phosphorus.[64][66] The use of these pigs would reduce the potential of water pollution since they excrete from 30 to 70.7% less phosphorus in manure depending upon the age and diet.[64][66] The lower concentrations of phosphorus in surface runoff reduces algal growth, because phosphorus is the limiting nutrient for algae.[64] Because algae consume large amounts of oxygen, it can result in dead zones for fish.

In 2011, Chinese scientists generated dairy cows genetically engineered with genes for human beings to produce milk that would be the same as human breast milk.[67] This could potentially benefit mothers who cannot produce breast milk but want their children to have breast milk rather than formula. Aside from milk production, the researchers claim these transgenic cows to be identical to regular cows.[68] Two months later scientists from Argentina presented Rosita, a transgenic cow incorporating two human genes, to produce milk with similar properties as human breast milk.[69] In 2012, researchers from New Zealand also developed a genetically engineered cow that produced allergy-free milk.[70]

In 2006, a pig was engineered to produce omega-3 fatty acids through the expression of a roundworm gene.[71]

Goats have been genetically engineered to produce milk with strong spiderweb-like silk proteins in their milk.[72]

Genetically modified fish have been developed with promoters driving an over-production of growth hormone for use in the aquaculture industry to increase the speed of development and potentially reduce fishing pressure on wild stocks. AquaBounty, a biotechnology company working on bringing a GM salmon to market, claims that their GM AquAdvantage salmon can mature in half the time it takes non-GM salmon and achieves twice the size.[73] AquaBounty has applied for regulatory approval to market their GM salmon in the US. As of May 2012 the application was still pending.[74] On 25 November 2013 Canada approved commercial scale production and export of GM Salmon eggs but they are not approved for human consumption in Canada.[75]

Human gene therapy

Gene therapy,[76] uses genetically modified viruses to deliver genes that can cure disease in humans. Although gene therapy is still relatively new, it has had some successes. It has been used to treat genetic disorders such as severe combined immunodeficiency,[77] and Leber's congenital amaurosis.[78] Treatments are also being developed for a range of other currently incurable diseases, such as cystic fibrosis,[79] sickle cell anemia,[80] Parkinson's disease,[81][82] cancer,[83][84][85] diabetes,[86] heart disease[87] and muscular dystrophy.[88] Current gene therapy technology only targets the non-reproductive cells meaning that any changes introduced by the treatment can not be transmitted to the next generation. Gene therapy targeting the reproductive cells—so-called "Germ line Gene Therapy"—is very controversial and is unlikely to be developed in the near future.

Insects

Fruit flies

In biological research, transgenic fruit flies (Drosophila melanogaster) are model organisms used to study the effects of genetic changes on development.[89] Fruit flies are often preferred over other animals due to their short life cycle, low maintenance requirements, and relatively simple genome compared to many vertebrates.

Mosquitoes

In 2010, scientists created "malaria-resistant mosquitoes" in the laboratory.[90][91][92] The World Health Organization estimated that Malaria killed almost one million people in 2008.[93] Genetically modified male mosquitoes containing a lethal gene have been developed in order to combat the spread of Dengue fever.[94] Aedes aegypti mosquitoes, the single most important carrier of dengue fever, were reduced by 80% in a 2010 trial of these GM mosquitoes in the Cayman Islands.[95][96] Between 50 and 100 million people are affected by Dengue fever every year and 40,000 people die from it.[97]

Bollworms

A strain of Pectinophora gossypiella (Pink bollworm) has been developed that contains a fluorescent marker in their DNA. This allows researchers to monitor bollworms that have been sterilized by radiation and released in order to reduce bollworm infestation.[97][98]

Aquatic life

Cnidarians

Cnidarians such as Hydra and the sea anemone Nematostella vectensis have become attractive model organisms to study the evolution of immunity and certain developmental processes. An important technical breakthrough was the development of procedures for generation of stably transgenic hydras and sea anemones by embryo microinjection.[99]

Fish

GM fish are used for scientific research and as pets, and are being considered for use as food and as aquatic pollution sensors.

Genetically engineered fish are widely used in basic research in genetics and development. Two species of fish, zebrafish and medaka, are most commonly modified because they have optically clear chorions (shells), rapidly develop, and the 1-cell embryo is easy to see and microinject with transgenic DNA.[100]

The GloFish is a patented[101] brand of genetically modified (GM) fluorescent zebrafish with bright red, green, and orange fluorescent color. Although not originally developed for the ornamental fish trade, it became the first genetically modified animal to become publicly available as a pet when it was introduced for sale in 2003.[102] They were quickly banned for sale in California.[103]

Genetically modified fish have been developed with promoters driving an over-production of "all fish" growth hormone for use in the aquaculture industry to increase the speed of development and potentially reduce fishing pressure on wild stocks. This has resulted in dramatic growth enhancement in several species, including salmon,[104] trout[105] and tilapia.[106] AquaBounty, a biotechnology company working on bringing a GM salmon to market, claims that their GM AquAdvantage salmon can mature in half the time it takes non-GM salmon and achieves twice the size.[73] AquaBounty has applied for regulatory approval to market their GM salmon in the US. As of December 2012 the application was still pending.[74][107]

Several academic groups have been developing GM zebrafish to detect aquatic pollution. The lab that originated the GloFish discussed above originally developed them to change color in the presence of pollutants, to be used as environmental sensors.[108][109] A lab at University of Cincinnati has been developing GM zebrafish for the same purpose,[110][111] as has a lab at Tulane University.[112]

Regulation

Main articles: Regulation of genetic engineering and Regulation of the release of genetically modified organisms

The regulation of genetic engineering concerns the approaches taken by governments to assess and manage the risks associated with the use of genetic engineering technology and the development and release of genetically modified organisms (GMO), including genetically modified crops and genetically modified fish. There are differences in the regulation of GMOs between countries, with some of the most marked differences occurring between the USA and Europe.[113] Regulation varies in a given country depending on the intended use of the products of the genetic engineering. For example, a crop not intended for food use is generally not reviewed by authorities responsible for food safety.[114] The European Union differentiates between approval for cultivation within the EU and approval for import and processing. While only a few GMOs have been approved for cultivation in the EU a number of GMOs have been approved for import and processing.[115] The cultivation of GMOs has triggered a debate about coexistence of GM and nonGM crops. Depending on the coexistence regulations incentives for cultivation of GM crops differ.[116]

Controversy

Main article: Genetically modified food controversies

There is controversy over GMOs, especially with regard to their use in producing food. The dispute involves consumers, biotechnology companies, governmental regulators, non-governmental organizations, and scientists. The key areas of controversy related to GMO food are whether GM food should be labeled, the role of government regulators, the effect of GM crops on health and the environment, the effect on pesticide resistance, the impact of GM crops for farmers, and the role of GM crops in feeding the world population.

There is broad scientific consensus that food on the market derived from GM crops poses no greater risk than conventional food.[117][118][119] No reports of ill effects have been documented in the human population from ingesting GM food.[120][121][122] Although labeling of GMO products in the marketplace is required in many countries, it is not required in the United States and no distinction between marketed GMO and non-GMO foods is recognized by the US FDA.

Advocacy groups such as Greenpeace, The Non-GMO Project and Organic Consumers Association say that risks of GM food have not been adequately identified and managed, and have questioned the objectivity of regulatory authorities. Opponents say that food derived from GMOs may be unsafe and propose it be banned, or at least labeled. They have expressed concerns about the objectivity of regulators and rigor of the regulatory process, about contamination of the non-GM food supply, about effects of GMOs on the environment and nature, and about the consolidation of control of the food supply in companies that make and sell GMOs.

Recognition of the originators of GM crops

On June 19, 2013 the leaders of the three research teams that first applied genetic engineering to crops, Robert T. Fraley of Monsanto; Marc Van Montagu of Ghent University in Belgium and founder of Plant Genetic Systems and Crop Design; and Mary-Dell Chilton of the University of Washington and Washington University in St. Louis and Syngenta, were awarded with the World Food Prize. The prize, of $250,000, is awarded to people who improve the "quality, quantity or availability" of food in the world. The three competing teams first presented their results in January 1983.[123]

See also

2

References

  1. ^ Cornell Chronicle, May 14, 1987, page 3.Biologists invent gun for shooting cells with DNA
  2. ^ Sanford JC et al (1987) Delivery of substances into cells and tissues using a particle bombardment process. Journal of Particulate Science and Technology 5:27-37.
  3. ^ Klein, TM et al (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70-73.
  4. ^ Lee LY, Gelvin SB (February 2008). "T-DNA binary vectors and systems". Plant Physiol. 146 (2): 325–332. doi:10.1104/pp.107.113001. OCLC 1642351. PMC 2245830. PMID 18250230.
  5. ^ Park F (October 2007). "Lentiviral vectors: are they the future of animal transgenesis?". Physiol. Genomics. 31 (2): 159–173. doi:10.1152/physiolgenomics.00069.2007. OCLC 37367250. PMID 17684037.
  6. ^ Clive Root (2007). Domestication. Greenwood Publishing Groups.
  7. ^ Daniel Zohary, Maria Hopf, Ehud Weiss (2012). Domestication of Plants in the Old World: The origin and spread of plants in the old world. Oxford University Press.{{cite book}}: CS1 maint: multiple names: authors list (link)
  8. ^ "Genome and genetics timeline - 1973". Genome news network.
  9. ^ Jackson, DA; Symons, RH; Berg, P (1 October 1972). "Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli". PNAS. 69 (10): 2904–2909. Bibcode:1972PNAS...69.2904J. doi:10.1073/pnas.69.10.2904. PMC 389671. PMID 4342968.
  10. ^ M. K. Sateesh (25 August 2008). Bioethics And Biosafety. I. K. International Pvt Ltd. pp. 456–. ISBN 978-81-906757-0-3. Retrieved 27 March 2013.
  11. ^ Jefferson R. A. Kavanagh T. A. Bevan M. W. (1987). "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants". The EMBO Journal. 6 (13): 3901–3907. ISSN 0261-4189. PMC 553867. PMID 3327686.
  12. ^ Nosowitz, Dan (15 September 2011) Suntory Creates Mythical Blue (Or, Um, Lavender-ish) Rose Popular Science, Retrieved 30 August 2012
  13. ^ a b c Phys.Org website. April 4, 2005 Plant gene replacement results in the world's only blue rose
  14. ^ Kyodo (11 September 2011 Suntory to sell blue roses overseas The Japan Times, Retrieved 30 August 2012
  15. ^ Wired Report 2011
  16. ^ Gasdaska JR et al (2003) Advantages of Therapeutic Protein Production in the Aquatic Plant Lemna. BioProcessing Journal Mar/Apr 2003 pp 49–56 [1]
  17. ^ (10 December 2012) Engineering algae to make complex anti-cancer 'designer' drug PhysOrg, Retrieved 15 April 2013
  18. ^ Büttner-Mainik, A., et al (2011): Production of biologically active recombinant human factor H in Physcomitrella. Plant Biotechnology Journal 9, 373–383. [2]
  19. ^ Baur, A., R. Reski, G. Gorr (2005): Enhanced recovery of a secreted recombinant human growth factor using stabilizing additives and by co-expression of human serum albumin in the moss Physcomitrella patens. Plant Biotech. J. 3, 331–340 [3]
  20. ^ Protalix website – technology platform
  21. ^ Gali Weinreb and Koby Yeshayahou for Globes May 2, 2012. FDA approves Protalix Gaucher treatment
  22. ^ EPA Reregistration Eligibility Decision (RED) Bacillus thuringiensis "The potential risk to humans from dietary, non-dietary and occupational exposures of the delta-endotoxins and most of the cellular components of Bacillus thuringiensis are considered negligible." (p 34) "As described in the environmental assessment, section III(C), there should be no unreasonable adverse effects on nontarget organisms, or ground or surface water contamination concerns, from the delta-endotoxins and most of the cellular components of Bacillus thuringiensis when used according to currently approved label rates." (p 34
  23. ^ http://www.agf.gov.bc.ca/pesticides/infosheets/bt.pdf. Retrieved 21 January 2011
  24. ^ Hope, Alan (3 April 2013), News in brief: The Bio Safety Council ...", Flanders Today, Page 2; In 2013, the Flemish Institute for Biotechnology was supervising a trial of 448 poplar trees genetically engineered to produce less lignin so that they would be more suitable for conversion into bio-fuels.
  25. ^ http://dtma.cimmyt.org. Retrieved 21 January 2011
  26. ^ Canadian Food Inspection Agency. DD2009-76: Determination of the Safety of Pioneer Hi-Bred Production Ltd.'s Soybean (Glycine max (L.) Merr.) Event 305423 Issued: 2009-04 [4]. Retrieved January 2011
  27. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1146/annurev-arplant-042110-103751, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1146/annurev-arplant-042110-103751 instead.
  28. ^ About Golden Rice International Rice Research Institute. Retrieved 20 August 2012
  29. ^ Shaista Naqvi et al. Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways PNAS April 27, 2009.
  30. ^ Crop plants – "green factories" for fish oils, Rothamsted Research 14-11-2013.
  31. ^ Successful high-level accumulation of fish oil omega-3 long chain polyunsaturated fatty acids in a transgenic oilseed crop, Ruiz-Lopez, Noemi et al., The Plant Journal, accepted article, DOI:10.1111/tpj.12378, 2013.
  32. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi: 10.1038/nbt0303-227, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi= 10.1038/nbt0303-227 instead.
  33. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1038/sj.embor.7400769, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1038/sj.embor.7400769 instead.
  34. ^ Prins, T.W. and Kok, E.J. (2010) Food and feed safety aspects of cisgenic crop plant varieties Report 2010.001, Project number: 120.72.667.01, RIKILT – Institute of Food Safety, Netherlands. Retrieved 6 September 2010.
  35. ^ Melo, Eduardo O. (2007). "Animal transgenesis: state of the art and applications". J. Appl. Genet. 48 (1): 47–61. doi:10.1007/BF03194657. PMID 17272861. Archived from the original (PDF) on 26 September 2009. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  36. ^ Leader, Benjamin (January 2008). "Protein therapeutics: a summary and pharmacological classification". Nature Reviews Drug Discovery. A guide to drug discovery. 7 (1): 21–39. doi:10.1038/nrd2399. PMID 18097458. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
    Leader 2008 — Fee required for access to full text.
  37. ^ Walsh, Gary (April 2005). "Therapeutic insulins and their large-scale manufacture". Appl. Microbiol. Biotechnol. 67 (2): 151–159. doi:10.1007/s00253-004-1809-x. PMID 15580495.
    Walsh 2005 — Fee required for access to full text.
  38. ^ Summers, Rebecca (24 April 2013) Bacteria churn out first ever petrol-like biofuel New Scientist, Retrieved 27 April 2013
  39. ^ Pipe, Steven W. (May 2008). "Recombinant clotting factors". Thromb. Haemost. 99 (5): 840–850. doi:10.1160/TH07-10-0593. PMID 18449413.
  40. ^ Bryant, Jackie; Baxter, Louise; Cave, Carolyn B.; Milne, Ruairidh; Bryant, Jackie (2007). Bryant, Jackie (ed.). "Recombinant growth hormone for idiopathic short stature in children and adolescents". Cochrane Database Syst Rev (3): CD004440. doi:10.1002/14651858.CD004440.pub2. PMID 17636758.
    Bryant 2007 — Fee required for access to full text.
  41. ^ Baxter L, Bryant J, Cave CB, Milne R (2007). Bryant, Jackie (ed.). "Recombinant growth hormone for children and adolescents with Turner syndrome". Cochrane Database Syst Rev (1): CD003887. doi:10.1002/14651858.CD003887.pub2. PMID 17253498.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  42. ^ Panesar, Pamit et al (2010) "Enzymes in Food Processing: Fundamentals and Potential Applications", Chapter 10, I K International Publishing House, ISBN 978-93-80026-33-6
  43. ^ EFSA (2012). Genetically modified animals. Europe: EFSA.http://www.efsa.europa.eu/en/topics/topic/gmanimals.htm.
  44. ^ Murray, Joo (20). Genetically modified animals. Canada: Brainwaving.http://www.brainwaving.com/2010/07/28/genetically-modified-animals/.
  45. ^ Jaenisch, R. and Mintz, B. (1974). "Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". Proc. Natl. Acad. Sci. 71 (4): 1250–1254. Bibcode:1974PNAS...71.1250J. doi:10.1073/pnas.71.4.1250. PMC 388203. PMID 4364530.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  46. ^ rudinko, larisa (20). Guidance for industry. USA: Center for veterinary medicine Link.
  47. ^ Sathasivam K, Hobbs C, Mangiarini L; et al. (June 1999). "Transgenic models of Huntington's disease". Philosophical Transactions of the Royal Society B. 354 (1386): 963–9. doi:10.1098/rstb.1999.0447. PMC 1692600. PMID 10434294. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
  48. ^ Spencer, L (12 May 2005). "Antibody Response to Aerosolized Transgenic Human Alpha1-Antitrypsin". New England Journal of Medicine. 352: 19. Retrieved 28 April 2011. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  49. ^ "The Nobel Prize in Chemistry 2008". The Official Web Site of the Nobel Foundation. Retrieved 31 August 2012.
  50. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 11808636, please use {{cite journal}} with |pmid=11808636 instead.
  51. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1002/mrd.10146, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1002/mrd.10146 instead.
  52. ^ Hogg, Chris (12 January 2006) Taiwan Breeds Green-Glowing Pigs BBC, Retrieved 31 August 2012
  53. ^ a b Staff (8 January 2008) Fluorescent Chinese pig passes on trait to offspring AFP, Retrieved 31 August 2012
  54. ^ a b Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 19895119, please use {{cite journal}} with |pmid=19895119 instead.
  55. ^ a b Randall S. et al (2008) Genetically Modified Pigs for Medicine and Agriculture Biotechnology and Genetic Engineering Reviews – Vol. 25, 245–266, Retrieved 31 August 2012
  56. ^ Staff (2006) NTU produces green fluorescent pigs for medical research Taiwan Central News Agency, Retrieved 31 August 2012
  57. ^ Wongsrikeao P, Saenz D, Rinkoski T, Otoi T, Poeschla E (2011). "Antiviral restriction factor transgenesis in the domestic cat". Nature Methods. 8 (10): 853–9. doi:10.1038/nmeth.1703. PMID 21909101.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  58. ^ Staff (3 April 2012) Biology of HIV National Institute of Allergy and Infectious Diseases, Retrieved 31 August 2012.
  59. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1038/nature08090, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1038/nature08090 instead.
  60. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1038/459515a, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1038/459515a instead.
  61. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1038/459492a, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1038/459492a instead.
  62. ^ Louis-Marie Houdebine (2009) Production of Pharmaceutical by transgenic animals. Comparative Immunology, Microbiology & Infectious Diseases 32(2): 107–121 [5]
  63. ^ Britt Erickson, 10 February 2009, for Chemical & Engineering News. FDA Approves Drug From Transgenic Goat Milk Accessed October 6, 2012
  64. ^ a b c d Guelph(2010). Enviropig. Canada: http://www.uoguelph.ca/enviropig/index.shtml/.
  65. ^ Schimdt, Sarah. Genetically engineered pigs killed after funding ends, Postmedia News, June 22, 2012. Accessed July 31, 2012.
  66. ^ a b Canada. "Enviropig — Environmental Benefits | University of Guelph". Uoguelph.ca. Retrieved 8 March 2010.
  67. ^ stevenson, heidi(2011). Scientists Use Human Genes in Animals, So Cows Produce Human-Like Milk—Or Do They? USA:http://www.gaia-health.com/articles401/000433-human-genes-cows-produce-human-milk.shtml/.
  68. ^ Classical Medicine Journal (14 April 2010). "Genetically modified cows producing human milk".
  69. ^ Yapp, Robin (11 June 2011). "Scientists create cow that produces 'human' milk". The Daily Telegraph. London. Retrieved 15 June 2012.
  70. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1073/pnas.1210057109, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1073/pnas.1210057109 instead.
  71. ^ Lai L; et al. (2006). "Generation of cloned transgenic pigs rich in omega-3 fatty acids" (PDF). Nature Biotechnology. 24 (4): 435–436. doi:10.1038/nbt1198. PMC 2976610. PMID 16565727. Retrieved 29 March 2009. {{cite journal}}: Unknown parameter |author-separator= ignored (help)
  72. ^ Zyga, Lisa(2010). Scientist bred goats that produce spider silk. Link.
  73. ^ a b {AquAdvantage salmon}[6]
  74. ^ a b Andrew Pollack for the New York Times. "An Entrepreneur Bankrolls a Genetically Engineered Salmon" Published: May 21, 2012. Accessed October 7, 2012
  75. ^ Goldenberg, Suzanne (25 November 2013). "Canada approves production of GM salmon eggs on commercial scale". The Guardian. Retrieved 26 November 2013.
  76. ^ Selkirk SM (October 2004). "Gene therapy in clinical medicine". Postgrad Med J. 80 (948): 560–70. doi:10.1136/pgmj.2003.017764. PMC 1743106. PMID 15466989.
  77. ^ Cavazzana-Calvo M, Fischer A (June 2007). "Gene therapy for severe combined immunodeficiency: are we there yet?". J. Clin. Invest. 117 (6): 1456–65. doi:10.1172/JCI30953. PMC 1878528. PMID 17549248.
  78. ^ Richards, Sabrina (6 November 2012) Gene Therapy Arrives in Europe The Scientist, Retrieved 15 April 2013
  79. ^ Rosenecker J, Huth S, Rudolph C (October 2006). "Gene therapy for cystic fibrosis lung disease: current status and future perspectives". Current Opinion in Molecular Therapeutics. 8 (5): 439–45. PMID 17078386.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  80. ^ Persons DA, Nienhuis AW (July 2003). "Gene therapy for the hemoglobin disorders". Curr. Hematol. Rep. 2 (4): 348–55. PMID 12901333.
  81. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1016/S1474-4422(11)70039-4, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1016/S1474-4422(11)70039-4 instead.
  82. ^ Gallaher, James Gene therapy 'treats' Parkinson's disease BBC News Health, 17 March 2011. Retrieved 24 April 2011
  83. ^ Urbina, Zachary (12 February 2013) Genetically Engineered Virus Fights Liver Cancer United Academics, Retrieved 15 February 2013
  84. ^ "Treatment for Leukemia Is Showing Early Promise". The New York Times. Associated Press. 11 August 2011. p. A15. Retrieved 21 January 2013.
  85. ^ Coghlan, Andy (26 March 2013) Gene therapy cures leukaemia in eight days The New Scientist, Retrieved 15 April 2013
  86. ^ Staff (13 February 2013) Gene therapy cures diabetic dogs New Scientist, Retrieved 15 February 2013
  87. ^ (30 April 2013) New gene therapy trial gives hope to people with heart failure British Heart Foundation, Retrieved 5 may 2013
  88. ^ Foster K, Foster H, Dickson JG (December 2006). "Gene therapy progress and prospects: Duchenne muscular dystrophy". Gene Ther. 13 (24): 1677–85. doi:10.1038/sj.gt.3302877. PMID 17066097.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  89. ^ First Transgenic Mice and Fruit Flies
  90. ^ Gallagher, James GM mosquitoes offer malaria hope BBC News, Health, 20 April 2011. Retrieved 22 April 2011
  91. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1371/journal.ppat.1001003, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1371/journal.ppat.1001003 instead.
  92. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1038/nature09937, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1038/nature09937 instead.
  93. ^ World Health Organization, Malaria, Key Facts Retrieved 22 April 2011
  94. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1073/pnas.1019295108, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1073/pnas.1019295108 instead.
  95. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1038/nbt.2019, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1038/nbt.2019 instead.
  96. ^ Staff (March 2011) Cayman demonstrates RIDL potential Oxitec Newsletter, March 2011. Retrieved 20 September 2011
  97. ^ a b Nicholls, Henry (14 September 2011) Swarm troopers: Mutant armies waging war in the wild The New Scientist. Retrieved 20 September 2011
  98. ^ Staff Pectinophora gossypiella (pink bollworm) OX1138 Oxitec. Retrieved 30 September 2011
  99. ^ Wittlieb J, Khalturin K, Lohmann JU, Anton-Erxleben F and Bosch TCG (2006). "Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis". Proc. Natl. Acad. Sci. U.S.A. 103 (16): 6208–6211. Bibcode:2006PNAS..103.6208W. doi:10.1073/pnas.0510163103. PMC 1458856. PMID 16556723.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  100. ^ Hackett, P.B., Ekker, S.E. and Essner, J.J. (2004) Applications of transposable elements in fish for transgenesis and functional genomics. Fish Development and Genetics (Z. Gong and V. Korzh, eds.) World Scientific, Inc., Chapter 16, 532–580.
  101. ^ Published PCT Application WO2000049150 "Chimeric Gene Constructs for Generation of Fluorescent Transgenic Ornamental Fish." National University of Singapore [7]
  102. ^ Eric Hallerman Glofish, The First GM Animal Commercialized: Profits amid Controversy. June, 2004. Accessed September 3, 2012.[8]
  103. ^ Schuchat S. (2003) Why GloFish won't glow in California. San Francisco Chronicle.[9]
  104. ^ Shao Jun Du et al. (1992) Growth Enhancement in Transgenic Atlantic Salmon by the Use of an "All Fish" Chimeric Growth Hormone Gene Construct. Nature Biotechnology 10, 176–181 [10]
  105. ^ Devlin RF et al (2001) Growth of domesticated transgenic fish. Nature 409, 781–782 [11]
  106. ^ Rahman MA et al. (2001) Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene. Journal of Fish Biology 59(1):62–78 [12]
  107. ^ Staff (26 December 2012) Draft Environmental Assessment and Preliminary Finding of No Significant Impact Concerning a Genetically Engineered Atlantic Salmon; Availability Federal Register / Vol. 77, No. 247 / Wednesday, December 26, 2012 / Notices, Retrieved 2 January 2013
  108. ^ National University of Singapore Enterprise webpage
  109. ^ Zebra Fish as Pollution Indicators Page last modified on 31 July 2001. Accessed October 2012
  110. ^ Carvan MJ et al (2000) Transgenic zebrafish as sentinels for aquatic pollution. Ann N Y Acad Sci. 2000;919:133–47 [13]
  111. ^ Nebert DW et al (2002) Use of Reporter Genes and Vertebrate DNA Motifs in Transgenic Zebrafish as Sentinels for Assessing Aquatic Pollution. Environmental Health Perspectives 110(1):A15 | January 2002 [14]
  112. ^ Mattingly CJ et al (2001) Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (Danio rerio). Environ Health Perspect. 2001 Aug;109(8):845–9 [15]
  113. ^ Attention: This template ({{cite doi}}) is deprecated. To cite the publication identified by doi:10.1126/science.285.5426.384, please use {{cite journal}} (if it was published in a bona fide academic journal, otherwise {{cite report}} with |doi=10.1126/science.285.5426.384 instead.
  114. ^ PotatoPro
  115. ^ Wesseler, J. and N. Kalaitzandonakes (2011): Present and Future EU GMO policy. In Arie Oskam, Gerrit Meesters and Huib Silvis (eds.), EU Policy for Agriculture, Food and Rural Areas. Second Edition, pp. 23–323 – 23-332. Wageningen: Wageningen Academic Publishers
  116. ^ Beckmann, V., C. Soregaroli, J. Wesseler (2011): Coexistence of genetically modified (GM) and non-modified (non GM) crops: Are the two main property rights regimes equivalent with respect to the coexistence value? In "Genetically modified food and global welfare" edited by Colin Carter, GianCarlo Moschini and Ian Sheldon, pp 201–224. Volume 10 in Frontiers of Economics and Globalization Series. Bingley, UK: Emerald Group Publishing
  117. ^ American Association for the Advancement of Science (AAAS), Board of Directors (2012). Legally Mandating GM Food Labels Could Mislead and Falsely Alarm Consumers
  118. ^ A decade of EU-funded GMO research (2001–2010) (PDF). Directorate-General for Research and Innovation. Biotechnologies, Agriculture, Food. European Union. 2010. doi:10.2777/97784. ISBN 978-92-79-16344-9. "The main conclusion to be drawn from the efforts of more than 130 research projects, covering a period of more than 25 years of research, and involving more than 500 independent research groups, is that biotechnology, and in particular GMOs, are not per se more risky than e.g. conventional plant breeding technologies." (p. 16)
  119. ^ Ronald, Pamela (2011). "Plant Genetics, Sustainable Agriculture and Global Food Security". Genetics. 188 (1): 11–20.
  120. ^ American Medical Association (2012). Report 2 of the Council on Science and Public Health: Labeling of Bioengineered Foods "Bioengineered foods have been consumed for close to 20 years, and during that time, no overt consequences on human health have been reported and/or substantiated in the peer-reviewed literature." (first page)
  121. ^ United States Institute of Medicine and National Research Council (2004). Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects. National Academies Press. Free full-text. National Academies Press. pp R9-10: "In contrast to adverse health effects that have been associated with some traditional food production methods, similar serious health effects have not been identified as a result of genetic engineering techniques used in food production. This may be because developers of bioengineered organisms perform extensive compositional analyses to determine that each phenotype is desirable and to ensure that unintended changes have not occurred in key components of food."
  122. ^ Key S, Ma JK, Drake PM (June 2008). "Genetically modified plants and human health". J R Soc Med. 101 (6): 290–8. doi:10.1258/jrsm.2008.070372. PMC 2408621. PMID 18515776. pp 292-293. "Foods derived from GM crops have been consumed by hundreds of millions of people across the world for more than 15 years, with no reported ill effects (or legal cases related to human health), despite many of the consumers coming from that most litigious of countries, the USA."{{cite journal}}: CS1 maint: multiple names: authors list (link)
  123. ^ Andrew Pollack (19 June 2013). "Executive at Monsanto Wins Global Food Honor". The New York Times. Retrieved 20 June 2013.

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