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File:Soluble Expression PACE.png

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English: A scheme for the engineering of higher soluble expression. POI = protein of interest. When the POI is properly folded, the T7n portion is exposed, allowing for the binding to the T7c portion to form a fully functional T7 RNAP. This T7 RNAP can then transcribe gIII. If the POI is misfolded, then the T7 RNAP doesn’t form, leading to no gIII transcription.
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Author Alexjli

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12 May 2020

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current03:50, 13 May 2020Thumbnail for version as of 03:50, 13 May 20201,139 × 788 (44 KB)AlexjliCross-wiki upload from wiki.riteme.site

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