File:Ramos cell trogocytosis.png
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Summary
| Description |
English: Volumetric reconstruction from confocal slices of a Ramos-RAW cell interface. RTX-Al488-coated (green), PKH26-labelled Ramos cells (red) were incubated with RAW cells for 45 minutes at 37°C. RAW cells were labelled with anti-CD11b-APC (cyan). The RAW cell has extensively trogocytosed both RTX and PKH26. Inset shows the dotted area above it without the PKH26 channel overlaid, revealing the concentration of RTX-Al488 at the cell-cell interface, otherwise depleted from the rest of the Ramos cell. Trogocytosis reaction was halted by fixation 45 min after co-incubation. Ramos cells are approximately 12 µm in diameter.
Español: Reconstrucción volumétrica de preparaciones para microscopía confocal de una interfaz entre una célula de Ramos y una célula RAW. Las células de Ramos fueron revestidas con RTX-Al488 (en verde) y marcadas con PKH26 (rojo). Posteriormente se incuban con células RAW durante 45 minutos a 37º. Las células RAW se marcan con anti-CD11b-APC (en cian). La célula RAW ha trogocitado ampliamente tanto el RTX como el PKH26. |
| Date | |
| Source | PLoS One |
| Author | Theodore Pham, Patricia Mero, James W. Booth. |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:22, 26 December 2011 | | 1,183 × 720 (287 KB) | Gustavocarra | {{Information |Description ={{en|1=Volumetric reconstruction from confocal slices of a Ramos-RAW cell interface. RTX-Al488-coated (green), PKH26-labelled Ramos cells (red) were incubated with RAW cells for 45 minutes at 37°C. RAW cells were labelled w |
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