File:RFG maleimide construction.svg
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Summary
| Description |
English: FSLs can be easily created from cysteine-containing peptides, proteins or any other thiols of biological interest. The mixing of a cysteine containing peptide with FSL-RFG(Maleimide), in 4MMF buffer, allows the peptide to react with the maleimide residue of the FSL (through the well-known Michael nucleophilic addition reaction). Following overnight incubation then vacuum drying, an FSL peptide is created. No purification is required. |
| Source | Own work |
| Author | Xiphiaz |
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Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License. |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 01:39, 10 May 2012 | | 1,052 × 744 (61 KB) | Xiphiaz | {{Information |Description ={{en|1=FSLs can be easily created from cysteine-containing peptides, proteins or any other thiols of biological interest. The mixing of a cysteine containing peptide with FSL-RFG(Maleimide), in 4MMF buffer, allows the pep... |
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