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File:Deacetoxycephalosporin-C synthase.svg

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Summary

Description
English: The deacetoxycephalosporin-C synthase (EC 1.14.20.1) is an enzyme that catalyzes the chemical reaction
penicillin N (Pen N) + 2-oxoglutarate + O2 deacetoxycephalosporin C (DAOC) + succinate + CO2 + H2O
 
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References

  • Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP (1992). "Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway". Proceedings of the Royal Society B 248 (1323): 283–9. DOI:10.1098/rspb.1992.0073. PMID 1354366.
  • Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ (2001). "Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)". J. Mol. Biol. 308 (5): 937–48. DOI:10.1006/jmbi.2001.4649. PMID 11352583.
  • Yeh WK, Ghag SK and Queener SW (1992). "Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C Function, evolution, refolding, and enzyme engineering". Ann. N.Y. Acad. Sci. 672: 396–408. DOI:10.1111/j.1749-6632.1992.tb32705.x.
  • Andersson I (1998). "Structure of a cephalosporin synthase". Nature. 394 (6695): 805–9. DOI:10.1038/29575. PMID 9723623.
  • Dotzlaf JE, Yeh WK (1989). "Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus". J. Biol. Chem. 264 (17): 10219–27. PMID 2656705.
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