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Bordetella

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Bordetella
Flagellated "Bordetella bronchiseptica"
Flagellated Bordetella bronchiseptica
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Pseudomonadota
Class: Betaproteobacteria
Order: Burkholderiales
Family: Alcaligenaceae
Genus: Bordetella
Moreno-López 1952
Species[2]

Bordetella (/ˌbɔːrdəˈtɛlə/) is a genus of small (0.2 – 0.7 μm), Gram-negative, coccobacilli bacteria of the phylum Pseudomonadota. Bordetella species, with the exception of B. petrii, are obligate aerobes, as well as highly fastidious, or difficult to culture. All species can infect humans. The first three species to be described (B. pertussis, B. parapertussis, B. bronchiseptica) are sometimes referred to as the 'classical species'. Two of these (B. pertussis and B. bronchiseptica) are also motile.[3][4]

There are about 16 different species of Bordetella likely descending from ancestors who lived in soil and/or water environments.[5] B. pertussis and occasionally B. parapertussis cause pertussis (whooping cough) in humans, and some B. parapertussis strains only colonize sheep.[5] It has also been known to cause bronchitis in cats and bronchopneumonia in pigs.[5]

B. bronchiseptica rarely infects healthy humans, though disease in immunocompromised patients has been reported.[6] B. bronchiseptica causes several diseases in other mammals, including kennel cough in dogs and atrophic rhinitis in pigs. Other members of the genus cause similar diseases in other mammals, and in birds (B. hinzii, B. avium).

The genus Bordetella is named after Jules Bordet.

Pathogenesis

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The three most common species of Bordetella are B. pertussis, B. parapertussis and B. bronchiseptica. These species are known to accumulate in the respiratory tracts of mammals. This is most commonly seen in human infants as a product of an illness known as whooping cough. The particular species responsible for this illness is B. pertussis, and can only be found in humans. Even with extensive vaccination research on B. pertussis, whooping cough is still considered endemic in many countries. Due to the fact B. pertussis is only found in humans and shows little genetic variation from the other Bordetella species, it is thought that it was derived from a common ancestor in recent years.

B. parapertussis can affect both humans and other mammals, primarily sheep. Similar to B. pertussis, it causes whooping cough in babies. Yet, when strains found in sheep are isolated there is a strong distinction between those found in humans. This suggests that the varying strains of this species evolved independently of one another, the one found in humans and the one found in sheep. With this particular distinction it means that there is little to no transmission between the two reservoirs.

The species B. bronchiseptica (gram-negative, aerobic) however has a broader host range, causing similar symptoms in a wide range of animals, while only occasionally affecting humans. These symptoms often manifest as chronic and asymptomatic respiratory infections. B. bronchiseptica is a small, coccobacillus shape sized at approximately 0.5 μm. It has peritrichous flagella that enables it to be motile. On a petri dish, colonies of this species appear small, grayish-white, smooth, and shiny. This species is also typically associated with kennel cough (Canine Respiratory Infectious Disease (CRID)) in dogs.[7][8]

Molecular structure of pertussis toxin complex (protein) highlighting alpha helices and beta pleated sheets. Also including the 5 different sub-units.

The most thoroughly studied of the Bordetella species are B. bronchiseptica, B. pertussis and B. parapertussis, and the pathogenesis of respiratory disease caused by these bacteria has been reviewed.[9][10][11] Transmission occurs by direct contact, via respiratory aerosol droplets, or fomites. Bacteria initially adhere to ciliated epithelial cells in the nasopharynx, and this interaction with epithelial cells is mediated by a series of protein adhesins.. These include filamentous haemaglutinin, pertactin, fimbriae, and pertussis toxin (though expression of pertussis toxin is unique to B. pertussis). As well as assisting in adherence to epithelial cells, some of these are also involved in attachment to immune effector cells.

The initial catarrhal phase of infection produces symptoms similar to those of the common cold, and during this period, large numbers of bacteria can be recovered from the pharynx. Thereafter, the bacteria proliferate and spread further into the respiratory tract, where the secretion of toxins causes ciliostasis and facilitates the entry of bacteria to tracheal/bronchial ciliated cells. One of the first toxins to be expressed is tracheal cytotoxin, which is a disaccharide-tetrapeptide derived from peptidoglycan. Unlike most other Bordetella toxins, tracheal cytotoxin is expressed constitutively, being a normal product of the breakdown of the bacterial cell wall. Other bacteria recycle this molecule back into the cytoplasm, but in Bordetella and Neisseria gonorrhoeae, it is released into the environment. Tracheal cytotoxin itself is able to reproduce paralysis of the ciliary escalator, inhibition of DNA synthesis in epithelial cells and ultimately killing of the same. One of the most important of the regulated toxins is adenylate cyclase toxin, which aids in the evasion of innate immunity. The toxin is delivered to phagocytic immune cells upon contact.[12] Immune cell functions are then inhibited in part by the resulting accumulation of cyclic AMP. Recently discovered activities of adenylate cyclase toxin, including transmembrane pore formation and stimulation of calcium influx, may also contribute to the intoxication of phagocytes.

Virulence factors

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The virulence factors identified in the Bordetella are common to all three species. These include adhesins, such as filamentous hemagglutinin (FHA), pertactin, tracheal colonization factor and fimbriae, and toxins, such as adenylate cyclase-hemolysin, dermonecrotic toxin and tracheal cytotoxin. These factors are then expressed and regulated most often by environmental stimuli. Differences in virulence factors relate to the loss of regulatory or control functions. Bordetella sp. is typically found to live within the hosts' respiratory tract and immune system and can transmit to new hosts.[5] Bordetella pertussis also affects human adults and even with an 85% vaccination coverage over 160,000 related deaths occur each year all around the globe.[5] There are few antimicrobial susceptibility testing methods but no change or progress have been discovered as of 2018.[5] Most studies performed using Bordetella vaccines have many flaws and fail to come to an official conclusion.

Regulation of virulence factor expression

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The expression of many Bordetella adhesins and toxins is controlled by the two-component regulatory system BvgAS.[10][11] Much of what is known about this regulatory system is based on work with B. bronchiseptica, but BvgAS is present in B. pertussis, B. parapertussis and B. bronchiseptica and is responsible for phase variation or phenotypic modulation.

BvgS is a plasma membrane-bound sensor kinase which responds to stimulation by phosphorylating a cytoplasmic helix-turn-helix-containing protein, BvgA. When phosphorylated, BvgA has increased affinity for specific binding sites in Bvg-activated promoter sequences and is able to promote transcription in in vitro assays.[13][14]

Most of the toxins and adhesins under BvgAS control are expressed under Bvg+ conditions (high BvgA-Pi concentration). But there are also genes expressed solely in the Bvg− state, most notably the flagellin gene flaA.[15] The regulation of Bvg repressed genes is mediated by the product of a 624-bp open reading frame downstream of BvgA, the so-called Bvg-activated repressor protein, BvgR.[16] BvgR binds to a consensus sequence present within the coding sequences of at least some Bvg-repressed genes. Binding of this protein to the consensus sequence represents gene expression by reducing transcription.[17]

It is not known what the physiological signals for BvgS are, but in vitro BvgAS can be inactivated by millimolar concentrations of magnesium sulfate or nicotinic acid, or by reduction of the incubation temperature to ≤ 26 °C.[18][19]

The identification of a specific point mutation in the BvgS gene which locks B. bronchiseptica in an intermediate Bvg phase revealed a class of BvgAS-regulated genes that are exclusively transcribed under intermediate concentrations of BvgA-Pi. This intermediate (Bvgi) phenotype can be reproduced in wild-type B. bronchiseptica by growth of the bacteria in a medium containing intermediate concentrations of the BvgAS modulator, nicotinic acid. In these conditions, some, but not all of the virulence factors associated with the Bvg+ phase are expressed, suggesting this two-component regulatory system can give rise to a continuum of phenotypic states in response to the environment.[18]

Vaccines

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The Bordetella vaccine is non-essential, but highly recommended for dogs especially if they are expected to come into contact with other dogs at dog parks, boarding facilities, dog shows, training classes, etc.[20] In fact, it can be required at certain facilities for entry. The vaccine can also be given to cats, but it is less commonly done because infection appears to be uncommon in adult cats. However, it may be a good idea to vaccinate a kitten if it is in a high-risk environment (i.e. living with multiple other cats).[21]

The Bordetella vaccine specifically targets Bordetella bronchiseptica, the species typically responsible for kennel cough. The vaccine introduces the bacteria (live or dead) to the body in order to develop an immunity. It is important to remember that the vaccine only protects against one species of Bordetella. Therefore, it is possible for a pet to become infected with another Bordetella species or contract kennel cough from another source, such as the parainfluenza virus, even after being vaccinated for B. bronchiseptica.[20] The Bordetella vaccine is also only about 70% effective.[22]

There are three licensed ways to deliver the Bordetella vaccine to dogs: orally, intranasally, and subcutaneously. The two former methods are administered using live bacteria, while the latter is done with a killed bacteria. A comparative study done in 2013 by the School of Veterinary Medicine in Madison, Wisconsin studied the effectiveness of these three methods by vaccinating beagle puppies. The 40 beagles were divided into four groups; a group to test each of the three methods, plus one unvaccinated control group. After 42 days, the dogs were exposed to Bordetella bronchiseptica. This study determined that the live intranasal Bordetella vaccine was more effective than the killed subcutaneous vaccine, and the live oral vaccine works equally as well as the live intranasal vaccine.[23]

References

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  1. ^ Tran TD, Ali MA, Lee D, Félix MA, Luallen RJ (4 February 2022). "Bacterial filamentation as a mechanism for cell-to-cell spread within an animal host". Nature Communications. 13 (1): 693. Bibcode:2022NatCo..13..693T. doi:10.1038/s41467-022-28297-6. PMC 8816909. PMID 35121734.
  2. ^ "Bordetella". NCBI taxonomy. Bethesda, MD: National Center for Biotechnology Information. Retrieved 16 January 2019.
  3. ^ Ryan KJ, Ray CG, eds. (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 978-0-8385-8529-0.
  4. ^ Hoffman CL, Gonyar LA, Zacca F, Sisti F, Fernandez J, Wong T, Damron FH, Hewlett EL (2019-06-25). "Bordetella pertussis Can Be Motile and Express Flagellum-Like Structures". mBio. 10 (3). American Society for Microbiology: e00787-19. doi:10.1128/mbio.00787-19. ISSN 2161-2129. PMC 6520453. PMID 31088927.
  5. ^ a b c d e f Kamanova J (2020). "Bordetella Type III Secretion Injectosome and Effector Proteins". Frontiers in Cellular and Infection Microbiology. 10: 466. doi:10.3389/fcimb.2020.00466. ISSN 2235-2988. PMC 7498569. PMID 33014891.
  6. ^ Bauwens J, Spach D, Schacker T, Mustafa M, Bowden R (1992). "Bordetella bronchiseptica pneumonia and bacteremia following bone marrow transplantation". J Clin Microbiol. 30 (9): 2474–75. doi:10.1128/jcm.30.9.2474-2475.1992. PMC 265527. PMID 1401019.
  7. ^ Fastrès A, Canonne MA, Taminiau B, Billen F, Garigliany MM, Daube G, Clercx C (2020-03-24). "Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction". Veterinary Research. 51 (1): 46. doi:10.1186/s13567-020-00769-x. ISSN 1297-9716. PMC 7092585. PMID 32209128.
  8. ^ Ellis JA (2015-04-01). "How well do vaccines for Bordetella bronchiseptica work in dogs? A critical review of the literature 1977–2014". The Veterinary Journal. 204 (1): 5–16. doi:10.1016/j.tvjl.2015.02.006. ISSN 1090-0233. PMID 25747699.
  9. ^ Hewlett E (1997). "Pertussis: current concepts of pathogenesis and prevention". Pediatr Infect Dis J. 16 (4 Suppl): S78–84. doi:10.1097/00006454-199704001-00002. PMID 9109161.
  10. ^ a b Cotter PA, Miller JF (2001). "Chapter 13 - Bordetella". In Groisman EA (ed.). Principles of Bacterial Pathogenesis. Academic Press. pp. 619–674. doi:10.1016/B978-012304220-0/50014-5. ISBN 978-0-12-304220-0.
  11. ^ a b Mattoo S, Cherry J (2005). "Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies". Clin Microbiol Rev. 18 (2): 326–82. doi:10.1128/CMR.18.2.326-382.2005. PMC 1082800. PMID 15831828.
  12. ^ Gray MC, Donato GM, Jones FR, Kim T, Hewlett EL (2004). "Newly secreted adenylate cyclase toxin is responsible for intoxication of target cells by Bordetella pertussis". Mol. Microbiol. 53 (6): 1709–19. doi:10.1111/j.1365-2958.2004.04227.x. PMID 15341649. S2CID 22884606.
  13. ^ Uhl M, Miller J (1994). "Autophosphorylation and phosphotransfer in the Bordetella pertussis BvgAS signal transduction cascade". Proc Natl Acad Sci USA. 91 (3): 1163–67. Bibcode:1994PNAS...91.1163U. doi:10.1073/pnas.91.3.1163. PMC 521474. PMID 8302847.
  14. ^ Steffen P, Goyard S, Ullmann A (1996). "Phosphorylated BvgA is sufficient for transcriptional activation of virulence-regulated genes in Bordetella pertussis". EMBO J. 15 (1): 102–09. doi:10.1002/j.1460-2075.1996.tb00338.x. PMC 449922. PMID 8598192.
  15. ^ Akerley B, Monack D, Falkow S, Miller J (1992). "The bvgAS locus negatively controls motility and synthesis of flagella in Bordetella bronchiseptica". J Bacteriol. 174 (3): 980–90. doi:10.1128/jb.174.3.980-990.1992. PMC 206178. PMID 1370665.
  16. ^ Merkel T, Stibitz S (1995). "Identification of a locus required for the regulation of bvg-repressed genes in Bordetella pertussis". J Bacteriol. 177 (10): 2727–36. doi:10.1128/jb.177.10.2727-2736.1995. PMC 176943. PMID 7751282.
  17. ^ Beattie D, Mahan M, Mekalanos J (1993). "Repressor binding to a regulatory site in the DNA coding sequence is sufficient to confer transcriptional regulation of the vir-repressed genes (vrg genes) in Bordetella pertussis". J Bacteriol. 175 (2): 519–27. doi:10.1128/jb.175.2.519-527.1993. PMC 196167. PMID 8419298.
  18. ^ a b Cotter P, Miller J (1997). "A mutation in the Bordetella bronchiseptica bvgS gene results in reduced virulence and increased resistance to starvation, and identifies a new class of Bvg-regulated antigens". Mol Microbiol. 24 (4): 671–85. doi:10.1046/j.1365-2958.1997.3821741.x. PMID 9194696. S2CID 285961.
  19. ^ van den Akker W (1997). "Bordetella bronchiseptica has a BvgAS-controlled cytotoxic effect upon interaction with epithelial cells". FEMS Microbiol Lett. 156 (2): 239–44. doi:10.1111/j.1574-6968.1997.tb12734.x. PMID 9513272.
  20. ^ a b Burke A (May 26, 2017). "5 Facts About the Bordetella Vaccine for Dogs". American Kennel Club. Retrieved 2023-04-21.
  21. ^ Gardiner J (2019-03-27). "Vaccination Guidelines for Dogs and Cats". Animal Health Topics / School of Veterinary Medicine. Retrieved 2023-04-21.
  22. ^ "How Bordetella Vaccines Work". HowStuffWorks. 2009-01-23. Retrieved 2023-04-21.
  23. ^ Larson, Laurie & Thiel, Bliss & Sharp, Patricia & Schultz, Ronald. (2013). "A Comparative Study of Protective Immunity Provided by Oral, Intranasal and Parenteral Canine Bordetella bronchiseptica Vaccines". International Journal of Applied Research in Veterinary Medicine. 11. 153–160.

[1] [2] [3] [4] [5]

  1. ^ Bryant, C. (2009, January 23). How Bordetella Vaccines Work. Retrieved November 18, 2020, from https://animals.howstuffworks.com/pets/pet-travel/bordetella-vaccine1.htm.
  2. ^ Burke, A. (2018, July 13). 5 Facts About the Bordetella Vaccine for Dogs. Retrieved October 22, 2020, from https://www.akc.org/expert-advice/health/facts-bordetella-vaccine-dogs/
  3. ^ Gardiner, J. (2020, August 11). Vaccination Guidelines for Dogs and Cats. Retrieved November 18, 2020, from https://healthtopics.vetmed.ucdavis.edu/health-topics/feline/vaccination-guidelines-dogs-and-cats
  4. ^ Kadlec, K., & Schwarz, S. (2018). Antimicrobial Resistance in Bordetella bronchiseptica (1062719807 809797198 L. Cavaco & 1062719808 809797198 J. Shen, Eds.). Antimicrobial Resistance in Bacteria from Livestock and Companion Animals, 365–375. doi:10.1128/9781555819804.ch16
  5. ^ Larson, Laurie & Thiel, Bliss & Sharp, Patricia & Schultz, Ronald. (2013). "A Comparative Study of Protective Immunity Provided by Oral, Intranasal and Parenteral Canine Bordetella bronchiseptica Vaccines". International Journal of Applied Research in Veterinary Medicine. 11. 153–160
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