Alcian yellow

Alcian yellow
Names
	IUPAC name {(E)-1,2-Diazenediylbis[4,1-phenylene(6-methyl-1,3-benzothiazole-2,5-diyl)methylenesulfanediyl]}bis[(dimethylamino)-N,N-dimethylmethaniminium]
	Other names Ingrain Yellow 1
Identifiers
CAS Number	61968-76-1;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:87352 ;
ChemSpider	2342179;
ECHA InfoCard	100.115.894
PubChem CID	3085232;
	InChI InChI=1S/C40H46N8S4/c1-25-19-35-33(21-29(25)23-49-39(45(3)4)46(5)6)41-37(51-35)27-11-15-31(16-12-27)43-44-32-17-13-28(14-18-32)38-42-34-22-30(26(2)20-36(34)52-38)24-50-40(47(7)8)48(9)10/h11-22H,23-24H2,1-10H3/q+2/b44-43+ Key: QMKQNEXRGZFFKQ-VGFSZAGXSA-N; InChI=1/C40H46N8S4/c1-25-19-35-33(21-29(25)23-49-39(45(3)4)46(5)6)41-37(51-35)27-11-15-31(16-12-27)43-44-32-17-13-28(14-18-32)38-42-34-22-30(26(2)20-36(34)52-38)24-50-40(47(7)8)48(9)10/h11-22H,23-24H2,1-10H3/q+2/b44-43+ Key: QMKQNEXRGZFFKQ-VGFSZAGXBQ;
	SMILES Cc1cc2c(cc1CSC(=[N+](C)C)N(C)C)nc(s2)c3ccc(cc3)/N=N/c4ccc(cc4)c5nc6cc(c(cc6s5)C)CSC(=[N+](C)C)N(C)C;
Properties
Chemical formula	C40H46N8S4
Molar mass	767.11 g/mol
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

Alcian yellow is an azo dye with the formula (CH₃[(CH₃N)₂CS]₂C₆H₂SNCC₆H₄)₂N₂. Its structure consists of a largely conjugated array of benzene, benzothiazole and isothiouronium units.

Histological Use

Alcian yellow is used in histology in conjunction with toluidine blue to help visualize H. pylori.^[1] The resulting structures are stained as follows:^[2]

– Mucin: yellow

– H. pylori: blue

– Other cellular components/background: shades of blue

References

^ Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining Method for Helicobacter Pylori in Gastric Biopsies". Journal of Histotechnology. 19 (2): 131–132. doi:10.1179/his.1996.19.2.131. ISSN 0147-8885.
^ "Helico*Stat Rapid Helicobacter Stain Kit Procedure" (PDF). Archived from the original (PDF) on 2017-03-06. Retrieved 2017-03-05.

[1] Leung, Jennifer K.; Gibbon, Kevin J.; Vartanian, Robert K. (1996-06-01). "Rapid Staining Method for Helicobacter Pylori in Gastric Biopsies". Journal of Histotechnology. 19 (2): 131–132. doi:10.1179/his.1996.19.2.131. ISSN 0147-8885.

[2] "Helico*Stat Rapid Helicobacter Stain Kit Procedure" (PDF). Archived from the original (PDF) on 2017-03-06. Retrieved 2017-03-05.

[1]

[2]

Histological Use

See also

References